产品简要
公司名称 :
MyBioSource
产品类型 :
ELISA/assay
产品名称 :
Human CXCL10/IP-10 PicoKine ELISA Kit
目录 :
MBS175853
规格 :
96-Strip-Wells
价格 :
475美元
更多信息或购买 :
图像
图像 1 :
MyBioSource MBS175853 图像 1
Human CXCL10/IP-10 PicoKine ELISA Kit standard curve
产品信息
目录号 :
MBS175853
产品类型 :
酶联免疫吸附试剂盒
产品全称 :
Human CXCL10/IP-10 PicoKine ELISA Kit
产品简称 :
[CXCL10/免疫沉淀-10]
产品名称同义词 :
[C-X-C motif chemokine 10; chemokine (C-X-C motif) ligand 10; 10 kDa interferon gamma-induced protein; C7; Chemokine (C X C motif) ligand 10; Chemokine CXC motif ligand 10; Crg 2; CRG2; CXCL10; CXCL10(1-73); CXL10_HUMAN; Gamma IP10; Gamma-IP10; gIP 10; GIP10; IFI10; INP 10; Interferon activated gene 10; Interferon gamma induced cell line; Interferon inducible cytokine IP10; IP 10; IP-10; MOB1; SCYB10; Small inducible cytokine B10; Small inducible cytokine B10 precursor; Small inducible cytokine subfamily B (Cys X Cys) member 10; Small inducible cytokine subfamily B CXC member 10; Small-inducible cytokine B10]
其他名称 :
[C-X-C motif chemokine 10; C-X-C motif chemokine 10; C-X-C motif chemokine 10; gamma IP10; gamma-IP10; small-inducible cytokine B10; interferon-inducible cytokine IP-10; 10 kDa interferon gamma-induced protein; protein 10 from interferon (gamma)-induced cell line; small inducible cytokine subfamily B (Cys-X-Cys), member 10; chemokine (C-X-C motif) ligand 10; 10 kDa interferon gamma-induced protein; Gamma-IP10; IP-10; Small-inducible cytokine B10Cleaved into the following chain:CXCL10(1-73)]
产品基因名称 :
[CXCL10]
其他基因名称 :
[CXCL10; CXCL10; C7; IFI10; INP10; IP-10; crg-2; mob-1; SCYB10; gIP-10; INP10; SCYB10; Gamma-IP10; IP-10]
UniProt数据库进入名 :
CXL10_HUMAN
反应物种 :
人类
序列长度 :
98
特异性 :
Natural and recombinant Human CXCL10/IP-10
储存稳定性 :
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
image1头 :
Typical Testing Data/Standard Curve (for reference only)
其它信息1 :
Samples: Serum, plasma(heparin, EDTA), saliva and urine. Assay Type: Quantitative Sandwich. Detection Range: 31.2pg/ml-2000pg/ml. Sensitivity: <1pg/ml
其他信息2 :
Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
产品描述 :
Principle of the Assay: The Human CXCL10 ELISA Kit was based on standard sandwich enzyme-linked immunesorbent assay technology. A monoclonal antibody from mouse specific for CXCL10 has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli; Immunogen sequence: V22-P98) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL10 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human CXCL10 amount of sample captured in plate. Background/Introduction: Chemokine(C-X-C motif) ligand 10(CXCL10) is a small cytokine belonging to the CXC chemokine family that is also known as 10 kDa interferon-gamma-induced protein(gamma-IP10). CXCL10 is secreted by several cell types in response to IFN-gamma. These cell types include monocytes, endothelial cells and fibroblasts. CXCL10 has been attributed to several roles, such as chemoattraction for monocytes/macrophages, T cells, NK cells, and dendritic cells, promotion of T cell adhesion to endothelial cells, antitumor activity, and inhibition of bone marrow colony formation and angiogenesis.It is a potent inhibitor of angiogenesis in vivo. The gene for CXCL10 is located on human chromosome 4 in a cluster among several other CXC chemokines. It is a RAS target gene and is overexpressed in the majority of colorectal cancers.This chemokine elicits its effects by binding to the cell surface chemokine receptor CXCR3.
NCBI GI登录号 :
21542456
NCBI登录号 :
P02778.2
UniProt数据库登录号 :
P02778
NCBI分子量 :
10,881 Da
NCBI信号通路 :
CXCR3-mediated Signaling Events Pathway (138011); Chemokine Receptors Bind Chemokines Pathway (106359); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Cytosolic DNA-sensing Pathway (125137); Cytosolic DNA-sensing Pathway (124833); G Alpha (i) Signalling Events Pathway (119550)
NCBI总结 :
This antimicrobial gene encodes a chemokine of the CXC subfamily and ligand for the receptor CXCR3. Binding of this protein to CXCR3 results in pleiotropic effects, including stimulation of monocytes, natural killer and T-cell migration, and modulation of adhesion molecule expression. [provided by RefSeq, Sep 2014]
UniProt数据库总结 :
CXCL10: Chemotactic for monocytes and T-lymphocytes. Binds to CXCR3. Belongs to the intercrine alpha (chemokine CxC) family. Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Chemokine; Secreted. Chromosomal Location of Human Ortholog: 4q21. Cellular Component: extracellular space; extracellular region; external side of plasma membrane. Molecular Function: heparin binding; protein binding; CXCR3 chemokine receptor binding; chemokine activity; cAMP-dependent protein kinase regulator activity; receptor binding. Biological Process: muscle development; blood circulation; protein secretion; positive regulation of leukocyte chemotaxis; positive regulation of cAMP metabolic process; chemotaxis; signal transduction; regulation of cell proliferation; G-protein coupled receptor protein signaling pathway; negative regulation of angiogenesis; response to vitamin D; cell surface receptor linked signal transduction; cell-cell signaling; positive regulation of cell proliferation; response to gamma radiation; positive regulation of transcription from RNA polymerase II promoter; immune response; positive regulation of release of sequestered calcium ion into cytosol; response to cold; inflammatory response; negative regulation of myoblast differentiation; regulation of protein kinase activity; defense response to virus
尺寸1 :
96-Strip-Wells
价格1 :
475美元
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。