MyBioSource

ELISA/assay

Human sL-Selectin PicoKine ELISA Kit

MBS175892

1 x96 Wells

410美元

MBS175892

酶联免疫吸附试剂盒

Human sL-Selectin PicoKine ELISA Kit

sL-Selectin

L-selectin; selectin L; A.11; AI528707; CD62 antigen ligand; CD62 antigen-like family member L; CD62L antigen; gp90 MEL; hLHRc; IgA nephropathy, susceptibility to, included; L Selectin; L selectin precursor; LAM 1; LAM1; LECAM1; Leu 8; Leu8; Leukocyte adhesion molecule 1; Leukocyte endothelial cell adhesion molecule 1; Leukocyte surface antigen Leu 8; Leukocyte surface antigen Leu8; Leukocyte-endothelial cell adhesion molecule 1; LNHR; LSEL; Ly-22; Ly-m22; Lyam 1; LYAM1; Lymph node homing receptor; Lymphocyte adhesion molecule 1; Lymphocyte antigen 22; Lymphocyte surface MEL-14 antigen; PLNHR; Selectin L; Selectin L (lymphocyte adhesion molecule 1); Selectin, lymphocyte; SELL; TQ1

L-selectin; L-selectin; L-selectin; gp90-MEL; pln homing receptor; lymph node homing receptor; lymphocyte adhesion molecule 1; leukocyte surface antigen Leu-8; CD62 antigen-like family member L; leukocyte-endothelial cell adhesion molecule 1; selectin L; CD62 antigen-like family member L; Leukocyte adhesion molecule 1; LAM-1; Leukocyte surface antigen Leu-8; Leukocyte-endothelial cell adhesion molecule 1; LECAM1; Lymph node homing receptor; TQ1; gp90-MEL; CD_antigen: CD62L

SELL

SELL;SELL;TQ1;LAM1;LEU8;LNHR;LSEL;CD62L;LYAM1;PLNHR;LECAM1;LNHR;LYAM1;LAM-1;LECAM1

LYAM1_HUMAN

人类

372

Natural and recombinant human sL-Selectin

Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Samples: For quantitative detection of human soluble L-Selectin in cell culture supernates, serum and plasma(heparin, EDTA, citrate). Detection Range: 62.5pg/ml-4000pg/ml

Sensitivity: < 5pg/ml

Principle of the assay: human sL-Selectin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for sL-Selectin has been precoated onto 96-well plates. Standards(NSO,W39 - N332) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for sL-Selectin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human sL-Selectin amount of sample captured in plate. Background: L-selectin, also known as CD62L, is a cell adhesion molecule found on leukocytes. It belongs to the selectin family of proteins, which recognize sialylated carbohydrate groups. It is cleaved by ADAM17.SELL (L-selectin) is a cell surface component that is a member of a family of adhesion/homing receptors which play important roles in leukocyte-endothelial cell interactions. The molecule is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins.1 L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to leukocyte expressing L-selectin, slowing leukocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point2.

126178

P14151.2

P14151

43,618 Da

Adaptive Immune System Pathway 366160!!Cell Adhesion Molecules (CAMs) Pathway 83069!!Cell Adhesion Molecules (CAMs) Pathway 480!!Cell Surface Interactions At The Vascular Wall Pathway 106062!!Hemostasis Pathway 106028!!Immune System Pathway 106386!!Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway 106413

This gene encodes a cell surface adhesion molecule that belongs to a family of adhesion/homing receptors. The encoded protein contains a C-type lectin-like domain, a calcium-binding epidermal growth factor-like domain, and two short complement-like repeats. The gene product is required for binding and subsequent rolling of leucocytes on endothelial cells, facilitating their migration into secondary lymphoid organs and inflammation sites. Single-nucleotide polymorphisms in this gene have been associated with various diseases including immunoglobulin A nephropathy. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2009]

1 x96 Wells

410美元