ELISA/assay

Rat TNF alpha PicoKine ELISA Kit

MBS175904

96 Wells

380美元

酶联免疫吸附试剂盒

Rat TNF alpha PicoKine ELISA Kit

肿瘤坏死因子甲

Tumor necrosis factor; tumor necrosis factor; APC1 protein; Cachectin; DIF; soluble form; Tnf; TNF a; TNF alpha; TNF macrophage derived; TNF monocyte derived; TNF superfamily member 2; TNF-a; TNF-alpha; TNFA_HUMAN; TNFSF2; Tumor necrosis factor; Tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2

Tumor necrosis factor; Tumor necrosis factor; tumor necrosis factor; cachectin; tumor necrosis factor alpha; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a

肿瘤坏死因子

Tnf; Tnf; Tnfa; RATTNF; TNF-alpha; Tnfa; Tnfsf2; TNF-a; NTF; ICD1; ICD2

TNFA_RAT

大鼠

235

Natural and recombinant rat TNFalpha

Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Samples: Cell culture supernates, serum and plasma (heparin, EDTA). Assay Type: Sandwich. Detection Range: 15.6pg/ml-1000pg/ml (Body fluids, tissue lysates or cell culture supernates), 7.8pg/ml-500pg/ml (Rat serum). Sensitivity: < 1pg/ml

Principle of the assay: rat TNFalpha ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TNFalpha has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFalpha is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat TNFalpha amount of sample captured in plate. Background: Tumor necrosis factor-alpha (TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer.1 Human Tumor Necrosis Factor (TNF) and Lymphotoxin (TNF-beta) are cytotoxic proteinswhich have similar biological activities and share 30% amino acid homology.2 TNF-alpha is produced by monocytes,which can stimulate endothelial cells to produce the multilineage growth factor granulocyte-macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro.3 TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNFhas been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids.4 TNF-alpha has been mapped to human chromosome 6.2

135938

P16599.1

P16599

25,806 Da

Adipocytokine Signaling Pathway (83485); Adipocytokine Signaling Pathway (505); Adipogenesis Pathway (198479); African Trypanosomiasis Pathway (194377); African Trypanosomiasis Pathway (194323); Allograft Rejection Pathway (83511); Allograft Rejection Pathway (535); Alzheimer's Disease Pathway (83489); Alzheimer's Disease Pathway (509); Amoebiasis Pathway (167311)

acts as a cytokine; binds TNF receptors; plays a role in regulation of cell proliferation, induction of apoptosis, and inflammatory response [RGD, Feb 2006]

TNF-a: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Homotrimer. Interacts with SPPL2B. Belongs to the tumor necrosis factor family. Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Cytokine; Apoptosis. Cellular Component: extracellular space; recycling endosome; cell surface; integral to plasma membrane; integral to membrane; plasma membrane; intracellular; external side of plasma membrane; phagocytic cup; lipid raft. Molecular Function: identical protein binding; protease binding; cytokine activity; tumor necrosis factor receptor binding. Biological Process: extracellular matrix organization and biogenesis; positive regulation of nitric oxide biosynthetic process; positive regulation of NFAT protein import into nucleus; activation of MAPK activity; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; positive regulation of caspase activity; positive regulation of translational initiation by iron; positive regulation of membrane protein ectodomain proteolysis; activation of NF-kappaB transcription factor; positive regulation of MAP kinase activity; cellular extravasation; tumor necrosis factor-mediated signaling pathway; positive regulation of phagocytosis; negative regulation of interleukin-6 production; JNK cascade; negative regulation of osteoblast differentiation; positive regulation of action potential; embryonic gut development; negative regulation of protein complex disassembly; response to drug; positive regulation of cytokine production; positive regulation of heterotypic cell-cell adhesion; positive regulation of mitosis; response to virus; positive regulation of interleukin-6 production; glucose metabolic process; negative regulation of cytokine secretion during immune response; positive regulation of protein transport; DNA damage response, signal transduction resulting in induction of apoptosis; response to mechanical stimulus; defense response to bacterium; positive regulation of transcription from RNA polymerase II promoter; skeletal muscle contraction; sequestering of triacylglycerol; positive regulation of JNK cascade; negative regulation of transcription from RNA polymerase II promoter; signal transduction; positive regulation of interleukin-18 production; chronic inflammatory response to antigenic stimulus; positive regulation of hair follicle development; negative regulation of cell proliferation; positive regulation of neuron apoptosis; protein kinase B signaling cascade; positive regulation of chronic inflammatory response to antigenic stimulus; inflammatory response; regulation of protein amino acid phosphorylation; transformed cell apoptosis; positive regulation of peptidyl-serine phosphorylation; humoral immune response; regulation of cell proliferation; positive regulation of protein kinase B signaling cascade; positive regulation of interferon-gamma production; positive regulation of programmed cell death; positive regulation of protein complex assembly; negative regulation of viral genome replication; response to hypoxia; regulation of insulin secretion; positive regulation of JNK activity; positive regulation of osteoclast differentiation; multicellular organismal development; response to glucocorticoid stimulus; response to lipopolysaccharide; positive regulation of NF-kappaB import into nucleus; osteoclast differentiation; regulation of immunoglobulin secretion; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-8 biosynthetic process; positive regulation of chemokine production; cell activation; detection of mechanical stimulus involved in sensory perception of pain; organ morphogenesis; defense response to Gram-positive bacterium; induction of apoptosis via death domain receptors; positive regulation of transcription factor activity; negative regulation of L-glutamate transport; response to activity; negative regulation of transcription, DNA-dependent; leukocyte migration; apoptosis; positive regulation of smooth muscle cell proliferation; defense response; positive regulation of synaptic transmission; response to radiation; regulation of protein secretion; regulation of osteoclast differentiation; negative regulation of lipid catabolic process; lipopolysaccharide-mediated signaling pathway; regulation of I-kappaB kinase/NF-kappaB cascade; caspase activation; positive regulation of humoral immune response mediated by circulating immunoglobulin; positive regulation of protein complex disassembly; calcium-mediated signaling; MAPKKK cascade; negative regulation of glucose import; positive regulation of chemokine biosynthetic process; protein import into nucleus, translocation; positive regulation of protein kinase activity; positive regulation of fever; activation of MAPKKK activity; positive regulation of protein amino acid phosphorylation; receptor biosynthetic process; leukocyte tethering or rolling; negative regulation of myoblast differentiation; positive regulation of inflammatory response; positive regulation of cytokine secretion

96 Wells

380美元