抗体

抗NADPH氧化酶4抗体

MBS176126

0.1毫克

315美元

多克隆

兔

未共轭

人类, 小鼠, 大鼠

免疫印迹, 免疫组化, 免疫组化-石蜡切片

抗体

抗NADPH氧化酶4抗体

[NADPH氧化酶4]

[NADPH oxidase 4; NADPH oxidase 4; Kidney oxidase-1 antibody; Kidney superoxide-producing NADPH oxidase antibody; KOX 1 antibody; KOX antibody; Kox-1 antibody; NADPH antibody; NADPH oxidase 4 antibody; Nox4 antibody; NOX4_HUMAN antibody; Renal NAD(P)H-oxidase antibody; RENOX antibody]

[NADPH oxidase 4; NADPH oxidase 4; NADPH oxidase 4; KOX-1; kidney oxidase-1; renal NAD(P)H-oxidase; superoxide-generating NADPH oxidase 4; kidney superoxide-producing NADPH oxidase; NADPH oxidase 4; Kidney oxidase-1; KOX-1; Kidney superoxide-producing NADPH oxidase; Renal NAD(P)H-oxidase; Superoxide-generating NADPH oxidase 4]

[NOX4]

[Nox4; Nox4; AI648021; Renox; KOX-1]

NOX4_MOUSE

多克隆

兔IgG

兔

Human, Rat; Predicted to work with: Mouse

578

No cross reactivity with other proteins

免疫原亲和纯化

Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg Thimerosal, 0.05mg NaN 3 .

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

免疫印迹(免疫印迹), 免疫组化(免疫组化)石蜡

Western Blot:. Concentration:0.1-0.5ug/ml. Tested Species: Hu, Rat. Predicted Species: Ms. Immunohistochemistry (Paraffin-embedded Section): . Concentration:0.5-1ug/ml . Tested Species: Rat . Predicted Species: Hu,Ms . Antigen Retrieval: By Heat. Tested Species: In house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested.

免疫印迹(免疫印迹)

免疫组化(免疫组化)

流式细胞仪(FC/流式细胞仪)

免疫印迹(免疫印迹)

Figure 4. Western blot analysis of NADPH oxidase 4/NOX4 using anti-NADPH oxidase 4/NOX4 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NADPH oxidase 4/NOX4 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. Specific bands were detected for NADPH oxidase 4/NOX4 at approximately 67-80KD. The expected band size for NADPH oxidase 4/NOX4 are at 67-80KD.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of mouse NOX4(561-578aa NRNNSYGTKFEYNKES), identical to the related rat sequence and different from the related human sequence by two amino acids.

Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.

NADPH oxidase 4 is an enzyme that in humans is encoded by the NOX4 gene, and is a member of the NOX family of NADPH oxidases. This gene encodes a member of the NOX-family of enzymes that functions as the catalytic subunit the NADPH oxidase complex. The encoded protein is localized to non-phagocytic cells where it acts as an oxygen sensor and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS). The ROS generated by this protein have been implicated in numerous biological functions including signal transduction, cell differentiation and tumor cell growth. A pseudogene has been identified on the other arm of chromosome 11. Alternative splicing results in multiple transcript variants

81881891

Q9JHI8.1

Q9JHI8

脊髓损伤通路(739004)

NOX4: Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation. Isoform 3 is not functional. Isoform 4 displays an increased activity. Isoform 5 and isoform 6 display reduced activity. 7 isoforms of the human protein are produced by alternative splicing. Protein type: Oxidoreductase; Nucleolus; EC 1.6.3.-; Membrane protein, multi-pass; Membrane protein, integral; Cell cycle regulation. Cellular Component: focal adhesion; mitochondrion; membrane; endoplasmic reticulum; perinuclear region of cytoplasm; apical plasma membrane; integral to membrane; stress fiber; plasma membrane; cell junction; NADPH oxidase complex. Molecular Function: NAD(P)H oxidase activity; superoxide-generating NADPH oxidase activity; oxidoreductase activity, acting on NADH or NADPH, with oxygen as acceptor; oxidoreductase activity. Biological Process: positive regulation of apoptosis; cell morphogenesis; superoxide metabolic process; cell aging; homocysteine metabolic process; positive regulation of smooth muscle cell migration; cardiac muscle cell differentiation; positive regulation of protein kinase B signaling cascade; positive regulation of MAP kinase activity; negative regulation of cell proliferation; positive regulation of stress fiber formation; superoxide release; bone resorption

0.1毫克

315美元