产品简要
公司名称 :
MyBioSource
产品类型 :
抗体
产品名称 :
抗半胱天冬酶1兔单克隆抗体
目录 :
MBS179060
规格 :
0.03毫克
价格 :
160美元
克隆性 :
单克隆
宿主 :
兔
共轭标签 :
未共轭
克隆名称 :
[DCB-3]
反应物种 :
人类, 大鼠
应用 :
免疫印迹, 免疫组化
更多信息或购买 :
MyBioSource 产品网页
图像
图像 1 :
MyBioSource MBS179060 图像 1
Immunohistochemical analysis of paraffin-embedded human liver, using Caspase-1 Antibody (MBS179060) CASP1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CASP1 Antibody (MBS179060) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (MBS176451) with DAB as the chromogen.
图像 2 :
MyBioSource MBS179060 图像 2
Western blot analysis of Caspase-1 in HeLa cell lysate (MBS179060). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CASP1 monoclonal antibody (MBS179060) overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) (MBS176460) kit with Tanon 5200 system. A specific band was detected for CASP1.
产品信息
目录号 :
MBS179060
产品类型 :
抗体
产品全称 :
抗半胱天冬酶1兔单克隆抗体
产品简称 :
[半胱天冬酶1]
产品名称同义词 :
[Caspase-1; CASP-1; 3.4.22.36; Interleukin-1 beta convertase; IL-1BC; Interleukin-1 beta-converting enzyme; ICE; IL-1 beta-converting enzyme; p45; Caspase-1 subunit p20; Caspase-1 subunit p10; CASP1; IL1BC; IL1BCE]
其他名称 :
[caspase-1 isoform beta; Caspase-1; caspase-1; caspase 1; Interleukin-1 beta convertase; IL-1BC]
产品基因名称 :
[胱天蛋白酶凋亡相关半胱氨酸肽酶1]
其他基因名称 :
[CASP1; CASP1; ICE; P45; IL1BC; IL1BC; IL1BCE; CASP-1; IL-1BC; ICE; IL-1 beta-converting enzyme]
克隆性 :
单克隆
克隆 :
[DCB-3]
宿主 :
兔
反应物种 :
人类, 大鼠
纯度 :
亲和色谱
形式 :
液体;兔IgG在磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油
浓度 :
0.5-1mg/ml, actual concentration varies by lot. Use suggested dilution ratio to decide dilution procedure.
储存稳定性 :
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
检测过的应用 :
免疫组化(免疫组化), 免疫印迹(免疫印迹)
应用笔记 :
WB: 1:500-1:2000。 IHC: 1:50-1:200
image1头 :
免疫组化(免疫组化)
image2头 :
免疫印迹(免疫印迹)
其它信息1 :
Immunogen: A synthesized peptide derived from human Caspase-1. Subcellular Localization: Cytoplasm. Tissue Specificity: Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain. Conjugate: No
产品种类 :
Cell Biology; Apoptosis; Intracellular; Caspases Etc; Caspases
产品描述 :
Anti-Caspase-1 Rabbit Monoclonal Antibody tested for IHC, WB in Human, Rat
NCBI GI登录号 :
4502573
NCBI登录号 :
NP_001214.1
NCBI基因登录号 :
NM_001223.4
UniProt数据库登录号 :
P29466
NCBI信号通路 :
Amyotrophic Lateral Sclerosis (ALS) Pathway (83099); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Apoptosis Pathway (198797); Caspase Cascade In Apoptosis Pathway (137974); Cellular Roles Of Anthrax Toxin Pathway (138076); Cytokine Signaling In Immune System Pathway (366171); Cytosolic DNA-sensing Pathway (125137); Cytosolic DNA-sensing Pathway (124833); Direct P53 Effectors Pathway (137939); IFN-gamma Pathway (138040)
NCBI总结 :
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq, Mar 2012]
UniProt数据库总结 :
Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, controls antiviral immunity through the cleavage of CGAS, rendering it inactive (PubMed:28314590).
尺寸1 :
0.03毫克
价格1 :
160美元
尺寸2 :
0.1毫克
价格2 :
340
size3 :
0.1 mg + Biotin Secondary (MBS176645)
价格3 :
340
size4 :
0.1 mg + HRP Secondary (MBS176703)
price4 :
340
更多信息或购买 :
MyBioSource 产品网页
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华，公司愿景是“生物研究试剂的源头”，现在位于圣地亚哥市。