产品简要
公司名称 :
MyBioSource
产品类型 :
抗体
产品名称 :
抗IRF5兔单克隆抗体
目录 :
MBS179503
规格 :
0.03毫克
价格 :
160美元
克隆性 :
单克隆
宿主 :
兔
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪, 流式细胞仪
更多信息或购买 :
图像
图像 1 :
Western blot analysis of IRF5 expression in THP-1 cell lysate (MBS179503). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRF5 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IRF5
产品信息
目录号 :
MBS179503
产品类型 :
抗体
产品全称 :
抗IRF5兔单克隆抗体
产品简称 :
[IRF5]
产品名称同义词 :
[干扰素调节因子5;IRF-5;IRF5]
其他名称 :
[interferon regulatory factor 5 isoform b; Interferon regulatory factor 5; interferon regulatory factor 5; interferon regulatory factor 5]
产品基因名称 :
[IRF5]
其他基因名称 :
[IRF5;IRF5;SLEB10;IRF-5]
克隆性 :
单克隆
宿主 :
兔
反应物种 :
人类, 小鼠, 大鼠
序列长度 :
147
纯度 :
亲和色谱
形式 :
液体
储存稳定性 :
Store at -20°C for one year. After reconstitution, at 4° C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing. Ships in Dry Ice.
检测过的应用 :
流式细胞仪(FC/流式细胞仪), 免疫沉淀(免疫沉淀), 免疫荧光(IF), 免疫组化(免疫组化), 免疫细胞化学(免疫细胞化学), 免疫印迹(免疫印迹)
应用笔记 :
WB: 1:500-1:1000。 IHC: 1:50-1:200。 免疫细胞化学/IF:1:100-1:500。 IP: 1:100。 FC/FACS: 1:50
image1头 :
免疫印迹(免疫印迹)
其它信息1 :
Immunogen: A synthesized peptide derived from human IRF5. Subcellular Localization: Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm.
其他信息2 :
Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Conjugate: No
产品种类 :
Immunology; Innate Immunity; Cytokines; interferons; Epigenetics and Nuclear Signaling; Transcription; polymerase associated factors; pol iii Transcription; Domain Families; HLH/Leucine Zipper; helix-turn-helix; Transcription Factors
产品描述 :
Transcription factor involved in the induction of interferons IFNA and INFB and inflammatory cytokines upon virus infection. Activated by TLR7 or TLR8 signaling.
NCBI GI登录号 :
291190719
NCBI登录号 :
NP_001092097.2
NCBI基因登录号 :
NM_001098627.3
UniProt数据库登录号 :
Q13568
NCBI分子量 :
56044分子量
NCBI信号通路 :
Apoptosis Pathway (198797); Cytokine Signaling In Immune System Pathway (366171); Direct P53 Effectors Pathway (137939); Immune System Pathway (106386); Interferon Signaling Pathway (187103); Interferon Alpha/beta Signaling Pathway (187104); Interferon Gamma Signaling Pathway (187106); Regulation Of Toll-like Receptor Signaling Pathway (920955); Senescence And Autophagy Pathway (198780); Toll-like Receptor Signaling Pathway (83076)
NCBI总结 :
This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Alternative promoter use and alternative splicing result in multiple transcript variants, and a 30-nt indel polymorphism (SNP rs60344245) can result in loss of a 10-aa segment. [provided by RefSeq, Dec 2016]
UniProt数据库总结 :
Transcription factor involved in the induction of interferons IFNA and INFB and inflammatory cytokines upon virus infection. Activated by TLR7 or TLR8 signaling.
尺寸1 :
0.03毫克
价格1 :
160美元
尺寸2 :
0.1毫克
价格2 :
340
size3 :
0.1 mg + Biotin Secondary (MBS176645)
价格3 :
340
size4 :
0.1 mg + HRP Secondary (MBS176703)
price4 :
340
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华，公司愿景是“生物研究试剂的源头”，现在位于圣地亚哥市。