产品简要
公司名称 :
MyBioSource
产品类型 :
抗体
产品名称 :
抗ARG1/精氨酸酶1兔单克隆抗体
目录 :
MBS179560
规格 :
0.03毫克
价格 :
160美元
克隆性 :
单克隆
宿主 :
兔
共轭标签 :
未共轭
克隆名称 :
[ADG-1]
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫沉淀
更多信息或购买 :
MyBioSource 产品网页
图像
图像 1 :
MyBioSource MBS179560 图像 1
Immunohistochemical analysis of paraffin-embedded human kidney, using ARG1 Antibody(MBS179560). ARG1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ARG1 Antibody (MBS179560)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(MBS176451) with DAB as the chromogen.
图像 2 :
MyBioSource MBS179560 图像 2
Western blot analysis of ARG1 expression in Mouse liver lysate (MBS179560). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARG1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ARG1
产品信息
目录号 :
MBS179560
产品类型 :
抗体
产品全称 :
抗ARG1/精氨酸酶1兔单克隆抗体
产品简称 :
[ARG1/精氨酸酶1]
产品名称同义词 :
[精氨酸酶1;3.5。 3.1;肝类型精氨酸酶;类型I精氨酸酶;ARG1]
其他名称 :
[arginase-1 isoform 2; Arginase-1; arginase-1; arginase 1; Liver-type arginase; Type I arginase]
产品基因名称 :
[ARG1]
其他基因名称 :
[ARG1;ARG1]
克隆性 :
单克隆
克隆 :
[ADG-1]
宿主 :
兔
反应物种 :
人类, 小鼠, 大鼠
序列长度 :
236
纯度 :
亲和色谱
形式 :
液体;兔IgG在磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油
浓度 :
0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
储存稳定性 :
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
检测过的应用 :
免疫沉淀(免疫沉淀), 免疫印迹(免疫印迹)
应用笔记 :
WB: 1:500-1:2000。 IP: 1:50
image1头 :
免疫组化(免疫组化)
image2头 :
免疫印迹(免疫印迹)
其它信息1 :
Immunogen: A synthesized peptide derived from human ARG1. Conjugate: No
产品种类 :
Neuroscience; Neurology Process; neuroendocrinology; gh regulation; Biochemicals; Product Range; just add water; Chemical Type; bioactive peptides; Pharmacology; Receptors & transporters; peptide Receptors; ghrelin; agonists
产品描述 :
Anti-ARG1 Rabbit Monoclonal Antibody tested for IP, WB in Human, Mouse, Rat
NCBI GI登录号 :
10947139
NCBI登录号 :
NP_000036.2
NCBI基因登录号 :
NM_000045.3
UniProt数据库登录号 :
P05089
NCBI信号通路 :
ATF-2 Transcription Factor Network Pathway (138006); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Arginine And Proline Metabolism Pathway (82957); Arginine And Proline Metabolism Pathway (323); Biosynthesis Of Amino Acids Pathway (790012); Biosynthesis Of Amino Acids Pathway (795174); IL4-mediated Signaling Events Pathway (137933); Metabolism Pathway (477135); Metabolism Of Amino Acids And Derivatives Pathway (106169)
NCBI总结 :
Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
UniProt数据库总结 :
Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys.
尺寸1 :
0.03毫克
价格1 :
160美元
尺寸2 :
0.1毫克
价格2 :
340
size3 :
0.1 mg + Biotin Secondary (MBS176645)
价格3 :
340
size4 :
0.1 mg + HRP Secondary (MBS176703)
price4 :
340
更多信息或购买 :
MyBioSource 产品网页
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华，公司愿景是“生物研究试剂的源头”，现在位于圣地亚哥市。