产品简要
公司名称 :
MyBioSource
产品类型 :
抗体
产品名称 :
抗乙III微管蛋白兔单克隆抗体
目录 :
MBS179753
规格 :
0.03毫克
价格 :
160美元
克隆性 :
单克隆
宿主 :
兔
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪, 流式细胞仪
更多信息或购买 :
MyBioSource 产品网页
图像
图像 1 :
MyBioSource MBS179753 图像 1
Immunohistochemical analysis of paraffin-embedded human thyroid cancer, using beta III Tubulin Antibody(MBS179753). TUBB3 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TUBB3 Antibody (MBS179753)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
图像 2 :
MyBioSource MBS179753 图像 2
Western blot analysis of beta III Tubulin expression in (1) HeLa cell lysate; (2) PC-12 cell lysate; (3) Mouse brain lysate; (4) Rat brain lysate with beta Tubulin III Antibody (MBS179753). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TUBB3 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TUBB3
产品信息
目录号 :
MBS179753
产品类型 :
抗体
产品全称 :
抗乙III微管蛋白兔单克隆抗体
产品简称 :
[乙III微管蛋白]
产品名称同义词 :
[微管蛋白乙3链;微管蛋白乙4链;微管蛋白乙III;Ⅲ型β微管蛋白;TUBB4]
其他名称 :
[tubulin beta-3 chain isoform 2; Tubulin beta-3 chain; tubulin beta-3 chain; tubulin beta 3 class III; Tubulin beta-4 chain; Tubulin beta-III]
产品基因名称 :
[Ⅲ型β微管蛋白]
其他基因名称 :
[Ⅲ型β微管蛋白;Ⅲ型β微管蛋白;CDCBM;FEOM3;TUBB4;CDCBM1;CFEOM3;乙4;CFEOM3A;TUBB4]
克隆性 :
单克隆
宿主 :
兔
反应物种 :
人类, 小鼠, 大鼠
序列长度 :
378
纯度 :
亲和色谱
形式 :
液体。 兔IgG在磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油
储存稳定性 :
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
检测过的应用 :
流式细胞仪(FC/流式细胞仪), 免疫沉淀(免疫沉淀), 免疫荧光(IF), 免疫组化(免疫组化), 免疫细胞化学(免疫细胞化学), 免疫印迹(免疫印迹)
应用笔记 :
WB: 1:5000-1:10000。 IHC: 1:50-1:200。 免疫细胞化学/IF:1:50-1:200。 IP: 1:50。 FC/FACS: 1:50
image1头 :
免疫组化(免疫组化)
image2头 :
免疫印迹(免疫印迹)
其它信息1 :
Immunogen: A synthesized peptide derived from human beta III Tubulin. Subcellular Localization: Cytoplasm, cytoskeleton. Tissue Specificity: Expression is primarily restricted to central and peripheral nervous system. Greatly increased expression in most cancerous tissues. Conjugate: No
产品描述 :
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance.
NCBI GI登录号 :
308235963
NCBI登录号 :
NP_001184110.1
NCBI基因登录号 :
NM_001197181.1
UniProt数据库登录号 :
Q13509
NCBI信号通路 :
Chaperonin-mediated Protein Folding Pathway (106242); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Cooperation Of Prefoldin And TriC/CCT In Actin And Tubulin Folding Pathway (106243); Diurnally Regulated Genes With Circadian Orthologs Pathway (198813); Formation Of Tubulin Folding Intermediates By CCT/TriC Pathway (106245); G Alpha (s) Signalling Events Pathway (119549); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Gap Junction Pathway (83072); Gap Junction Pathway (483)
NCBI总结 :
This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Oct 2010]
UniProt数据库总结 :
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance.
尺寸1 :
0.03毫克
价格1 :
160美元
尺寸2 :
0.1毫克
价格2 :
340
size3 :
0.1 mg + Biotin Secondary (MBS176645)
价格3 :
340
size4 :
0.1 mg + HRP Secondary (MBS176703)
price4 :
340
更多信息或购买 :
MyBioSource 产品网页
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华，公司愿景是“生物研究试剂的源头”，现在位于圣地亚哥市。