产品简要
公司名称 :
MyBioSource
产品类型 :
抗体
产品名称 :
Anti-CD3 epsilon Rabbit Monoclonal Antibody
目录 :
MBS179879
规格 :
0.03毫克
价格 :
160美元
克隆性 :
单克隆
宿主 :
兔
共轭标签 :
未共轭
克隆名称 :
[EED-3]
反应物种 :
人类
应用 :
免疫印迹, 免疫组化, 免疫沉淀, 流式细胞仪, 流式细胞仪
更多信息或购买 :
图像
图像 1 :
Western blot analysis of CD3 epsilon expression in Jurkat cell lysate (MBS179879). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD3E monoclonal antibody overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD3E.
图像 2 :
IHC analysis of CD3 epsilon using anti- CD3 epsilon antibody (MBS179879) on human colon cancer. CD3 epsilon was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- CD3 epsilon Antibody (MBS179879) overnight at 4°C. Biotinylated goat anti Rabbit IgG IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (MBS176451) with DAB as the chromogen.
产品信息
目录号 :
MBS179879
产品类型 :
抗体
产品全称 :
Anti-CD3 epsilon Rabbit Monoclonal Antibody
产品简称 :
[CD3 epsilon]
产品名称同义词 :
[T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; CD3e; CD3E; T3E]
其他名称 :
[T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; CD3e molecule; T-cell surface antigen T3/Leu-4 epsilon chain; CD_antigen: CD3e]
产品基因名称 :
[CD3E]
其他基因名称 :
[CD3E;CD3E;T3E;TCRE;IMD18;T3E]
克隆性 :
单克隆
抗体亚型 :
IgG
克隆 :
[EED-3]
宿主 :
兔
反应物种 :
人类
序列长度 :
207
特异性 :
The His tag Ab can recognize C-terminal, internal, and Nterminal His-tag fusion proteins.
纯度 :
亲和色谱
形式 :
液体;磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油
浓度 :
0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
储存稳定性 :
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
检测过的应用 :
流式细胞仪(FC/流式细胞仪), 免疫沉淀(免疫沉淀), 免疫组化(免疫组化), 免疫印迹(免疫印迹)
应用笔记 :
WB: 1:500-1:2000。 IHC: 1:50-1:200。 IP: 1:30。 FC/FACS: 1:100
image1头 :
免疫印迹(免疫印迹)
image2头 :
免疫组化(免疫组化)
产品种类 :
Immunology; Adaptive Immunity; T Cells; Stem Cells; Hematopoietic Progenitors; Hematopoietic Stem Cells; Human Lineage Negative; Immunology; Regulatory T Cells
产品描述 :
The CD3 complex mediates signal transduction.
NCBI GI登录号 :
4502671
NCBI登录号 :
NP_000724.1
NCBI基因登录号 :
NM_000733.3
UniProt数据库登录号 :
P07766
NCBI信号通路 :
Adaptive Immune System Pathway (366160); CXCR4-mediated Signaling Events Pathway (137910); Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795); Costimulation By The CD28 Family Pathway (119552); Downstream TCR Signaling Pathway (106418); Downstream Signaling In Naive CD8+ T Cells Pathway (138018); Generation Of Second Messenger Molecules Pathway (106417); HTLV-I Infection Pathway (373901); HTLV-I Infection Pathway (373889)
NCBI总结 :
The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]
UniProt数据库总结 :
Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell developement. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed:10384095, PubMed:26507128).
尺寸1 :
0.03毫克
价格1 :
160美元
尺寸2 :
0.1毫克
价格2 :
340
size3 :
0.1 mg + Biotin Secondary (MBS176645)
价格3 :
340
size4 :
0.1 mg + HRP Secondary (MBS176703)
price4 :
340
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华，公司愿景是“生物研究试剂的源头”，现在位于圣地亚哥市。