抗体

Anti-K48-linkage Specific Ubiquitin Rabbit Monoclonal Antibody

MBS179903

0.03毫克

160美元

单克隆

兔

未共轭

免疫印迹, 免疫细胞化学, 流式细胞仪, 流式细胞仪

抗体

Anti-K48-linkage Specific Ubiquitin Rabbit Monoclonal Antibody

[K48-linkage Specific Ubiquitin]

[多聚泛肽B;泛肽;UBB]

[多聚泛肽B;多聚泛肽B;多聚泛肽B;泛肽B]

[UBB]

[UBB;UBB;HEL-S-50]

单克隆

兔

人类, 小鼠, 大鼠

229

亲和色谱

液体。 兔IgG在磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油

Store at -20°C for one year. After reconstitution, at 4° C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

流式细胞仪(FC/流式细胞仪), 免疫荧光(IF), 免疫细胞化学(免疫细胞化学), 免疫印迹(免疫印迹)

WB: 1:100-1:500。 免疫细胞化学/IF:1:50-1:200。 FC/FACS: 1:50

免疫印迹(免疫印迹)

Immunogen: A synthesized peptide derived from human K48-linkage Specific Ubiquitin. Subcellular Localization: Ubiquitin: Cytoplasm. Nucleus.

Conjugate: No

Neuroscience; Neurology Process; Neurodegenerative Disease; Alzheimer's Disease; tangles Epigenetics and Nuclear Signaling; Chromatin Modifying Enzymes; ubiquitylation; Cell Biology; Proteolysis/Ubiquitin; Proteasome/Ubiquitin; neuroscience

Ubiquitin: Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.

528524471

NP_001268645.1

NM_001281716.1

P0CG47

ABC Transporter Disorders Pathway (1268933); ABC-family Proteins Mediated Transport Pathway (1269904); APC-Cdc20 Mediated Degradation Of Nek2A Pathway (1269847); APC/C-mediated Degradation Of Cell Cycle Proteins Pathway (1269838); APC/C:Cdc20 Mediated Degradation Of Cyclin B Pathway (1269848); APC/C:Cdc20 Mediated Degradation Of Securin Pathway (1269849); APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway (1269844); APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway (1269851); APC:Cdc20 Mediated Degradation Of Cell Cycle Proteins Prior To Satisfation Of The Cell Cycle Checkpoint Pathway (1269845); ARMS-mediated Activation Pathway (1269471)

This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. An aberrant form of this protein has been detected in patients with Alzheimer's disease and Down syndrome. Pseudogenes of this gene are located on chromosomes 1, 2, 13, and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Ubiquitin: Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.

0.03毫克

160美元

0.1毫克

340

0.1 mg + Biotin Secondary (MBS176645)

340

0.1 mg + HRP Secondary (MBS176703)

340