抗体

抗泛肽单克隆抗体

MBS190525

0.1毫克

320美元

单克隆

小鼠

未共轭

5B9.B3

人类, 小鼠, 大鼠, 牛

免疫印迹, 酶联免疫吸附测定

抗体

抗泛肽单克隆抗体

泛肽

ubiquitin; Ubiquitin-40S ribosomal protein S27a; ubiquitin-40S ribosomal protein S27a; ubiquitin C; ubiquitin-CEP80; OTTHUMP00000200786; OTTHUMP00000200788; OTTHUMP00000200789; OTTHUMP00000201692; ubiquitin and ribosomal protein S27a; ubiquitin carboxyl extension protein 80; ribosomal protein S27a; Ubiquitin carboxyl extension protein 80

RPS27A;RPS27A;UBC;S27A;CEP80;UBA80;UBCEP1;UBCEP80;UBA80;UBCEP1

RS27A_HUMAN

单克隆

IgG2a

5B9.B3

小鼠

人类, 小鼠, 大鼠, 牛

泛肽。 这抗体识别人类, 小鼠, 大鼠, 和牛泛肽

100ug in PBS, pH 7.4, purified by Protein G affinity chromatography.

This antibody is stable for at least one (1) year at -20 degree C. Avoid repeated freezing and thawing.

酶联免疫吸附测定;免疫印迹

Immunoblotting: use at 1ug/ml. A band of ~10 kDa is detected. ELISA: use at 1ug/ml. These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: HeLa cell lysate

Antigen: Native bovine ubiquitin. Preservative: None

Dilution Instructions: Dilute in PBS or medium which is identical to that used in the assay system.

Ubiquitin, a small protein comprised of 76 amino acids with a molecular mass of approximately 10 kDa, is highly conserved among eukaryotic species. The primary role of ubiquitin is to clear abnormal, foreign, and improperly folded proteins by targeting them for degradation by the 26S proteosome. Ubiquitination of proteins is an essential process that is regulated by ubiquitinactivating enzymes, ubiquitinconjugating enzymes, and ubiquitinprotein ligases. Ubiquitination also occurs in the internalization and degradation of plasma membrane proteins.

37571

P62979

17,965 Da

APC/C-mediated Degradation Of Cell Cycle Proteins Pathway (105825); APC/C:Cdc20 Mediated Degradation Of Cyclin B Pathway (105832); APC/C:Cdc20 Mediated Degradation Of Securin Pathway (105833); APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway (105831); APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway (105835); Activated TLR4 Signalling Pathway (106400); Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway (105829); Activation Of The MRNA Upon Binding Of The Cap-binding Complex And EIFs, And Subsequent Binding To 43S Pathway (105970); Adaptive Immunity Signaling Pathway (366160); Antigen Processing: Ubiquitination Proteasome Degradation Pathway (366162)

Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a. Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin; similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi. Multiple alternatively spliced transcript variants that encode the same proteins have been identified.[provided by RefSeq]

RPS27A: the gene (RPS27A) that encodes this protein is one of four that encode for ubiquitin: UBC, UBB, UBA52 and RPS27A. UBB and UBC genes code for a polyubiquitin precursor with exact head to tail repeats, the number of repeats differ between species and strains. UBA52 and RPS27A genes code for a single copy of ubiquitin fused to the ribosomal proteins L40 and S27a, respectively. The RPS27A gene product is cleaved into the following 2 chains: ubiquitin (amino acids 1-76) and the 40S ribosomal protein S27a (amino acids 77-156). Ubiquitin is a peptide 76 amino acids in length that can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Hundreds of ubiquitin ligases and hydrolases have been identified, implicating ubiquitin as a major regulatory element in many crucial cellular systems. It can be covalently bound to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. At the protein level, it is not possible to easily determine which of the four genes encoded a given ubiquitin chain. Protein type: Ubiquitin-like modifier; Ribosomal. Chromosomal Location of Human Ortholog: 2p16. Cellular Component: nucleoplasm; small ribosomal subunit; membrane; cytoplasm; nucleolus; plasma membrane; endosome membrane; cytosol. Molecular Function: structural constituent of ribosome; metal ion binding. Biological Process: circadian rhythm; I-kappaB kinase/NF-kappaB cascade; SRP-dependent cotranslational protein targeting to membrane; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; nerve growth factor receptor signaling pathway; viral reproduction; positive regulation of apoptosis; activation of MAPK activity; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; endosome transport; T cell receptor signaling pathway; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; activation of NF-kappaB transcription factor; regulation of apoptosis; toll-like receptor 5 signaling pathway; antigen processing and presentation of peptide antigen via MHC class I; transforming growth factor beta receptor signaling pathway; translational initiation; antigen processing and presentation of exogenous peptide antigen via MHC class I; JNK cascade; viral transcription; G2/M transition of mitotic cell cycle; toll-like receptor 4 signaling pathway; regulation of interferon type I production; glycogen biosynthetic process; fibroblast growth factor receptor signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; Notch receptor processing; glucose metabolic process; virus assembly; toll-like receptor 2 signaling pathway; translational elongation; carbohydrate metabolic process; viral protein processing; mRNA catabolic process, nonsense-mediated decay; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; negative regulation of interferon type I production; negative regulation of apoptosis; G1/S transition of mitotic cell cycle; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of epidermal growth factor receptor signaling pathway; translation; apoptosis; pathogenesis; translational termination; viral infectious cycle; negative regulation of transcription from RNA polymerase II promoter; toll-like receptor 10 signaling pathway; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; positive regulation of interferon type I production; transmembrane transport; epidermal growth factor receptor signaling pathway; transcription initiation from RNA polymerase II promoter; Notch signaling pathway; cytokine and chemokine mediated signaling pathway; MyD88-independent toll-like receptor signaling pathway; DNA repair; MyD88-dependent toll-like receptor signaling pathway; cellular protein metabolic process; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; toll-like receptor signaling pathway; innate immune response; gene expression; mitotic cell cycle; negative regulation of transforming growth factor beta receptor signaling pathway

0.1毫克

320美元