抗体

Retinoblastoma (Rb) phosphothreonine 821Polyclonal Antibody

MBS194347

0.025毫克

395美元

多克隆

兔

未共轭

抗体

Retinoblastoma (Rb) phosphothreonine 821Polyclonal Antibody

视网膜母细胞瘤磷酸苏氨酸821

视网膜母细胞瘤磷酸苏氨酸821多克隆抗体

retinoblastoma; Retinoblastoma-associated protein; retinoblastoma-associated protein; OTTHUMP00000018397; retinoblastoma suspectibility protein; retinoblastoma 1; p105-Rb; pRb; Rb; pp110

RB1; RB1; RB; pRb; OSRC; pp110; p105-Rb

RB_HUMAN

多克隆

兔

Retinoblastoma phosphothreonine 821 polyclonal antibody [Rabbit polyclonal antibody specific for the phosphorylated form of human retinoblastoma protein at threonine 821. Useful in WB. Available as affinity purified antibody.]. This antibody reacts with human Rb protein phosphorylated at serine 821. Mouse andrat (100% conserved) have not been tested.

Sequential immunoaffinity-purified antibody in phosphate buffer, pH 7.4

Store antibody at -80 degree C. Upon initial thawing, aliquot in appropriate aliquots and store at -80 degree C. Avoid multiple freeze-thaw cycles.

免疫印迹

Western Blotting: 0.25-1.0 ug/ml is recommended. The optimal antibody concentration should be determined for each specific application.

Antigen: Phosphopeptide region of human Rb containing serine 821

Positive Control: Jurkat cell lysate in high growth phase. Stabilizer: 1 mg/ml BSA. Preservatives: 0.05% Sodium Azide

Cancer Research Products

29123599

P06400

106,159 Da

Adipogenesis Pathway (198832); Androgen Receptor Signaling Pathway (198806); B Cell Receptor Signaling Pathway (198909); Bladder Cancer Pathway (83115); Bladder Cancer Pathway (527); Cell Cycle, Mitotic Pathway (105765); Cell Cycle Pathway (198811); Cell Cycle Pathway (83054); Cell Cycle Pathway (463); Chronic Myeloid Leukemia Pathway (83116)

The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq]

Rb: retinoblastoma tumor suppressor protein regulates cell proliferation by controlling progression through the G1-phase restriction point. Has three distinct binding domains and interacts with regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase and proteins with a conserved LXCXE motif. Cell cycle-dependent phosphorylation by Cdks inhibits Rb target binding, thus allowing cell cycle progression. Recruits and targets histone methyltransferase suv39h1 leading to epigenetic transcriptional repression. Protein type: Tumor suppressor; Nuclear receptor co-regulator; Oncoprotein; Transcription, coactivator/corepressor. Chromosomal Location of Human Ortholog: 13q14.2. Cellular Component: nucleoplasm; SWI/SNF complex; Rb-E2F complex; PML body; spindle; nucleus; chromatin. Molecular Function: identical protein binding; protein binding; DNA binding; androgen receptor binding; ubiquitin protein ligase binding; transcription coactivator activity; phosphoprotein binding; kinase binding; transcription factor activity; transcription factor binding. Biological Process: viral reproduction; sister chromatid biorientation; negative regulation of transcription from RNA polymerase II promoter, mitotic; positive regulation of transcription, DNA-dependent; regulation of mitotic cell cycle; neuron maturation; cell cycle arrest; positive regulation of macrophage differentiation; neurite development; negative regulation of epithelial cell proliferation; transcription, DNA-dependent; negative regulation of transcription factor activity; mitotic cell cycle checkpoint; regulation of lipid kinase activity; enucleate erythrocyte differentiation; chromatin remodeling; myoblast differentiation; G1/S-specific transcription in mitotic cell cycle; neuron apoptosis; maintenance of mitotic sister chromatid cohesion; gut development; cell division; neuron morphogenesis during differentiation; negative regulation of smoothened signaling pathway; Ras protein signal transduction; androgen receptor signaling pathway; positive regulation of mitotic metaphase/anaphase transition; striated muscle cell differentiation; negative regulation of protein kinase activity; positive regulation of transcription from RNA polymerase II promoter; mitotic cell cycle; negative regulation of transcription, DNA-dependent; cell cycle checkpoint; G1/S transition of mitotic cell cycle. Disease: Bladder Cancer; Osteogenic Sarcoma; Small Cell Cancer Of The Lung; Retinoblastoma

0.025毫克

395美元