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nucleophosmin (nucleolar phosphoprotein B23; numatrin); B23; NPM; MGC104254; nucleolar phosphoprotein B23; numatrin; nucleophosmin/nucleoplasmin family; member 1
nucleophosmin isoform 1; Nucleophosmin; nucleophosmin; nucleolar protein NO38; nucleophosmin/nucleoplasmin family, member 1; nucleophosmin (nucleolar phosphoprotein B23, numatrin); Nucleolar phosphoprotein B23; Nucleolar protein NO38; Numatrin
By protein-G affinity chromatography
Can be stored at 4 degree C. For long term storage, aliquot and store at -20 degree C. Avoid repeated freezing and thawing cycles.
ELISA (EIA), Western Blot (WB) (Cell lysate), Immunohistochemistry (IHC)
The antibody has been tested by ELISA, Western blot and Immunohistochemical analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000 ~ 2,000. Recommended starting dilution is 1:1,000.
Antigen Species: Human. Clone: Anti-human NPM mAb, clone 5E3, is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human NPM protein. Immunogen: Recombinant human NPM (81-294aa) purified from E Coli
Nucleophosmin (NPM; also known as B23, NO38, or numatrin) is a nucleolar phosphoprotein that binds the tumor suppressors p53 and p19Arf and is thought to be indispensable for ribogenesis, cell proliferation, and survival after DNA damage. Nucleophosmin is upregulated in many cancer cells.
Maiguel DA et al., (2004) Mol cell Biol 24:3703-3711 Colombo Emanuela et al., (2005) Mol cell Biol 25:8874-8886
NCBI GI登录号 :
Aurora B Signaling Pathway (138080); BARD1 Signaling Events Pathway (137959); Cell Cycle Pathway (530733); Chromosome Maintenance Pathway (161048); Deposition Of New CENPA-containing Nucleosomes At The Centromere Pathway (161050); Disease Pathway (530764); HIF-1-alpha Transcription Factor Network Pathway (138045); HIV Infection Pathway (105983); Host Interactions Of HIV Factors Pathway (106010); Interactions Of Rev With Host Cellular Proteins Pathway (106014)
This gene encodes a phosphoprotein which moves between the nucleus and the cytoplasm. The gene product is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners have been characterized, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in this gene are associated with acute myeloid leukemia. More than a dozen pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Nov 2009]
NPM1: a nucleolar protein associated with nucleolar ribonucleoprotein structures and that binds single-stranded nucleic acids. Is a major component of template activating factor (TAF)-III. It plays a role in controlling centrosome duplication, and may function in the assembly and/or transport of the ribosome. Two alternatively spliced isoforms have been described. Protein type: Translation; RNA-binding; Oncoprotein; Nucleolus. Chromosomal Location of Human Ortholog: 5q35.1. Cellular Component: nucleoplasm; centrosome; focal adhesion; membrane; cytoplasm; nucleolus; spindle pole centrosome; cytosol; nucleus; ribonucleoprotein complex. Molecular Function: NF-kappaB binding; protein binding; protein homodimerization activity; protein kinase inhibitor activity; histone binding; Tat protein binding; protein heterodimerization activity; RNA binding; transcription coactivator activity; unfolded protein binding; protein kinase binding; ribosomal large subunit binding; ribosomal small subunit binding. Biological Process: regulation of centriole replication; positive regulation of translation; viral reproduction; centrosome cycle; cell aging; nucleocytoplasmic transport; signal transduction; DNA repair; protein oligomerization; activation of NF-kappaB transcription factor; nucleosome assembly; negative regulation of cell proliferation; intracellular protein transport; regulation of endodeoxyribonuclease activity; protein localization; DNA replication-independent nucleosome assembly at centromere; ribosome assembly; response to stress; negative regulation of apoptosis
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San Diego, CA 92195-3308
San Diego, CA 92195-3308