免疫印迹, 酶联免疫吸附测定, 酶免疫法
GIP2; uCRP; IFI15; ISG15; Interferon-stimulated gene; 15 kDa ubiquitin-like modifier; Interferon-stimulated gene; 15 kDa ubiquitin-like modifier IFI 15; Interferon alpha inducible protein; Interferon induced 15 kDa protein; Interferon induced 17 kDa protein; Interferon induced 17 kDa/15 kDa protein; Interferon Stimulated Gene 15; Interferon stimulated protein 15 kDa; ISG 15; ISG15 ubiquitin like modifier; ubiquitin cross reactive protein precursor.
ubiquitin-like protein ISG15; Ubiquitin-like protein ISG15; ubiquitin-like protein ISG15; interferon, alpha-inducible protein (clone IFI-15K); interferon-induced 17-kDa/15-kDa protein; interferon-stimulated protein, 15 kDa; ubiquitin cross-reactive protein; ISG15 ubiquitin-like modifier; Interferon-induced 15 kDa protein; Interferon-induced 17 kDa protein; IP17; Ubiquitin cross-reactive protein; hUCRP
By protein-G affinity chromatography
Can be stored at 4 degree C. For long term storage, aliquot and store at -20 degree C. Avoid repeated freezing and thawing cycles.
ELISA (EIA), Western Blot (WB) (Cell lysate)
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000 ~ 2,000. Recommended starting dilution is 1:1,000.
Antigen Species: Human. Clone: Anti-human ISG15 mAb, clone 3E5, is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human ISG15 protein. Immunogen: Recombinant human ISG15 (1-157aa) purified from E Coli
ISG15, a 15 kDa protein encoded by an interferon stimulated gene, and its modification system play important roles in the innate immune response, regulation of interferon signaling, pregnancy, and several cancers. ISG15 is an ubiquitin-like protein containing two ubiquitin homology domains and becomes conjugated to a variety of proteins when cells are treated with type I interferon or lipopolysaccharide.
Jonathan D et al.,(1996) Proc Natl Acad Sci USA 93:211-215 Weiming Y et al.,(2001) EMBO J 20(3):362-371 Chen Z et al.,(2005) Proc Natl Acad Sci USA 102(29):10200-10205
NCBI GI登录号 :
Antiviral Mechanism By IFN-stimulated Genes Pathway (530760); Cytokine Signaling In Immune System Pathway (366171); ISG15 Antiviral Mechanism Pathway (530761); Immune System Pathway (106386); Innate Immune System Pathway (106387); Interferon Signaling Pathway (187103); Interferon Alpha/beta Signaling Pathway (187104); Negative Regulators Of RIG-I/MDA5 Signaling Pathway (187102); RIG-I-like Receptor Signaling Pathway (117292); RIG-I-like Receptor Signaling Pathway (116128)
The protein encoded by this gene is a ubiquitin-like protein that is conjugated to intracellular target proteins upon activation by interferon-alpha and interferon-beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted. [provided by RefSeq, Dec 2012]
ISG15: Ubiquitin-like protein that is conjugated to intracellular target proteins after IFN-alpha or IFN-beta stimulation. Its enzymatic pathway is partially distinct from that of ubiquitin, differing in substrate specificity and interaction with ligating enzymes. ISG15 conjugation pathway uses a dedicated E1 enzyme, but seems to converge with the Ub conjugation pathway at the level of a specific E2 enzyme. Targets include STAT1, SERPINA3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, EIF2AK2/PKR, MX1/MxA, and RIG-1. Deconjugated by USP18/UBP43. Shows specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic factors. May serve as a trans-acting binding factor directing the association of ligated target proteins to intermediate filaments. May also be involved in autocrine, paracrine and endocrine mechanisms, as in cell-to-cell signaling, possibly partly by inducing IFN-gamma secretion by monocytes and macrophages. Seems to display antiviral activity during viral infections. Interacts with, and is conjugated to its targets by the UBE1L (E1 enzyme) and UBE2E2 (E2 enzyme) (Probable). Interaction with influenza B NS1 protein inhibits this conjugation. By type I interferons. Detected in lymphoid cells, striated and smooth muscle, several epithelia and neurons. Protein type: Ubiquitin-like modifier. Chromosomal Location of Human Ortholog: 1p36.33. Cellular Component: extracellular region; cytosol. Molecular Function: protein tag; protein binding. Biological Process: modification-dependent protein catabolic process; ISG15-protein conjugation; viral reproduction; negative regulation of viral genome replication; regulation of interferon-gamma production; positive regulation of erythrocyte differentiation; cytokine and chemokine mediated signaling pathway; defense response to bacterium; innate immune response; negative regulation of protein ubiquitination; defense response to virus; negative regulation of interferon type I production. Disease: Immunodeficiency 38
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San Diego, CA 92195-3308
San Diego, CA 92195-3308