抗体

Hsp27抗体(克隆2A5)

MBS200046

0.05毫升

240美元

单克隆

小鼠

未共轭

2A5

免疫印迹, 酶联免疫吸附测定, 免疫组化, 酶免疫法

抗体

Hsp27抗体(克隆2A5)

Hsp27

Hspb1; Hsp25; Hsp27; Hsp27; HspB1; Heat shock 27 kDa protein; HSP 27; Growth-related 25 kDa protein; P25; HSP25; Heat shock protein beta-1; Heat shock 27 kDa protein 28 kDa heat shock protein; CMT2F; DKFZp586P1322; Estrogen regulated 24 kDa protein; at shock 25kDa protein 1; Heat shock 27kDa protein 1; Heat shock 28kDa protein 1; Heat Shock Protein 27; Heat shock protein beta 1; Heat Shock Protein27; HS.76067; Hsp 28; Hsp B1; Hsp28; HspB1; SRP27; Stress responsive protein 27.

heat shock protein beta-1; Heat shock protein beta-1; heat shock protein beta-1; 28 kDa heat shock protein; HSP 27; epididymis secretory protein Li 102; estrogen-regulated 24 kDa protein; heat shock 27 kDa protein; heat shock 27kD protein 1; stress-responsive protein 27; heat shock 27kDa protein 1; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27

Hsp27

HSPB1;HSPB1;CMT2F;HMN2B;HSP27;HSP28;Hsp25;SRP27;HS.76067;HEL-S-102;HSP27;HSP28;HspB1;HSP 27;SRP27

HSPB1_HUMAN

单克隆

IgG1, k

2A5

小鼠

人类

205

By protein-G affinity chromatography

液体。 用品在磷酸盐缓冲液(pH值7.4)含0.1％叠氮化钠

1毫克/毫升

Can be stored at 4 degree C. For long term storage, aliquot and store at -20 degree C. Avoid repeated freezing and thawing cycles.

ELISA (EIA), Western Blot (WB) (Cell lysate), Immunohistochemistry (IHC)

The antibody has been tested by ELISA, Western blot and immunohistochemistry analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot is 1:1,000~1:2,000 and immunohistochemistry analysis is 1:50~100. Recommended starting dilution for Western blot is 1:1,000 and Immunohistochemistry is 1:50.

Antigen Species: Human. Clone: Anti-human Hsp27 mAb, clone 2A5, is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Hsp27 protein. Immunogen: Recombinant human Hsp27 (1-205aa) purified from E Coli

热休克蛋白

Hsp27 (also known as the Estrogen-Regulated 24k protein, and hsp 28) is a member of the mammalian small heat shock protein family. Hsp27 is expressed constitutively in many tissues and its expression is increased to high levels after various types of stress including elevated temperatures, toxic metals, drugs and oxidants. Also, Hsp27 is phosphorylated in vivo on three phosphorylation sites (Ser15, Ser78 and Ser82) by protein kinases including MAPkAP kinase 2 and the stress-activated protein kinase SAPk2 (p38). Hsp27 forms a multimeric complex within cells, and its primary chaperone function, like other small Hsps, appears to be the stabilization of denatured or aggregated proteins in a refolding competent state.

Ehrnsperger M, et al., (1997) EMBO J 16:221-229 Steve J, et al., (2000) Mol Cell Biol 20(20):7602-7612 Butt E, et al., (2001) J Biol Chem 276:7108-7113 Michael, et al., (2002) Cell Stress Chsperones 7(3):281-296

4504517

NP_001531

NM_001540.3

22,783 Da

AUF1 (hnRNP D0) Destabilizes MRNA Pathway 187212!!Amoebiasis Pathway 167324!!Amoebiasis Pathway 167191!!Epstein-Barr Virus Infection Pathway 585562!!Epstein-Barr Virus Infection Pathway 587115!!FAS Pathway And Stress Induction Of HSP Regulation 198894!!Gene Expression Pathway 105937!!IL-3 Signaling Pathway 198881!!IL-6 Signaling Pathway 198864!!MAPK Signaling Pathway 198779

The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq, Oct 2008]

0.05毫升

240美元