抗体

Rab5a抗体(克隆3A4)

MBS200066

0.05毫升

240美元

单克隆

小鼠

未共轭

3A4

人类, 小鼠

免疫印迹, 酶联免疫吸附测定, 酶免疫法

抗体

Rab5a抗体(克隆3A4)

Rab5a

RAB5A; member RAS oncogene family; RAB5; Rab5a; RAB5A; member RAS oncogene family RAB 5; RAB 5A; RAB5A member RAS oncogene family; RAS associated protein RAB5A; Ras related protein Rab 5A.

ras-related protein Rab-5A isoform 1; Ras-related protein Rab-5A; ras-related protein Rab-5A; RAS-associated protein RAB5A; RAB5A, member RAS oncogene family

Rab5a

RAB5A;RAB5A;RAB5;RAB5

RAB5A_HUMAN

单克隆

IgG2b, k

3A4

小鼠

人类, 小鼠

215

By protein-G affinity chromatography

液体。 用品在磷酸盐缓冲液(pH值7.4)含0.1％叠氮化钠

1毫克/毫升

Can be stored at 4 degree C. For long term storage, aliquot and store at -20 degree C. Avoid repeated freezing and thawing cycles.

ELISA (EIA), Western Blot (WB) (Cell lysate)

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1,000. Recommended starting dilution is 1:500.

Antigen Species: Human. Clone: Anti-human Rab5a mAb, clone 3A4, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Rab5a protein. Immunogen: Recombinant human Rab5a (1-215aa) purified from E Coli

Cancer

Rab5a is a member of the RAS oncogene family. Rab5a is a small GTPase localized to the plasma membrane and early endosomes. It is a crucial regulatory component of the endocytic pathway. Activation of Rab5a is mediated by GDP-GTP exchange factors (GEFs) that generate the Rab5-GTP complex.

Bucci C, et al., (1992) Cell 70 (5): 715-28 Seachrist JL, et al., (2002) J. Biol. Chem. 277 (1): 679-85

19923262

NP_004153

NM_004162.4

22,178 Da

Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Amyotrophic Lateral Sclerosis (ALS) Pathway (920975); Amyotrophic Lateral Sclerosis (ALS) Pathway (83099); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); EGFR1 Signaling Pathway (198782); Endocytosis Pathway (102279); Endocytosis Pathway (102181); ErbB1 Downstream Signaling Pathway (138057); Factors Involved In Megakaryocyte Development And Platelet Production Pathway (187196)

RAB5A: Required for the fusion of plasma membranes and early endosomes. Contributes to the regulation of filopodia extension. Binds EEA1. Interacts with RIN1 and GAPVD1, which regulate its pathway, probably by acting as a GEF. Interacts with ALS2CL, RABEP1, SUN2, ZFYVE20 and RUFY1. Interacts with SGSM1 and SGSM3. Interacts with PIK3CB. Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP. Belongs to the small GTPase superfamily. Rab family. Protein type: G protein, monomeric, Rab; G protein, monomeric; Motility/polarity/chemotaxis; G protein. Chromosomal Location of Human Ortholog: 3p24-p22. Cellular Component: zymogen granule membrane; protein complex; dendrite; early endosome; terminal button; phagocytic vesicle; cytosol; actin cytoskeleton; lipid raft; ruffle; synaptic vesicle; recycling endosome; cell soma; axon; perinuclear region of cytoplasm; endocytic vesicle; cytoplasm; melanosome; plasma membrane; endosome membrane; nerve terminal; endosome. Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding; GDP-dissociation inhibitor binding. Biological Process: regulation of long-term neuronal synaptic plasticity; intracellular protein transport; regulation of endosome size; early endosome to late endosome transport; positive regulation of smooth muscle cell proliferation; regulation of endocytosis; endocytosis; regulation of filopodium formation; positive regulation of smooth muscle cell migration; blood coagulation; Rab protein signal transduction; positive regulation of exocytosis

0.05毫升

240美元

0.1毫升

315