抗体

CD73抗体(克隆2B6)

MBS200071

0.05毫升

240美元

单克隆

小鼠

未共轭

2B6

免疫印迹, 酶联免疫吸附测定, 酶免疫法

抗体

CD73抗体(克隆2B6)

CD73

5' nucleotidase; ecto; 5'-nucleotidase ecto (CD73); 5' nucleotidase (CD73); CD73; eN; eNT

5'-nucleotidase isoform 1 preproprotein; 5'-nucleotidase; 5'-nucleotidase; 5'-NT; Purine 5-Prime-Nucleotidase; ecto-5'-nucleotidase; 5'-nucleotidase, ecto (CD73); Ecto-5'-nucleotidase; CD_antigen: CD73

CD73

NT5E; NT5E; NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA; NT5; NTE; 5'-NT

5NTD_HUMAN

单克隆

IgG2b, k

2B6

小鼠

人类

574

By protein-G affinity chromatography

液体。 用品在磷酸盐缓冲液(pH值7.4)含0.1％叠氮化钠

1毫克/毫升

Can be stored at 4 degree C. For long term storage, aliquot and store at -20 degree C. Avoid repeated freezing and thawing cycles.

ELISA (EIA), Western Blot (WB) (Cell lysate)

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000 ~ 2,000. Recommended starting dilution is 1:1,000.

Antigen Species: Human. Clone: Anti-human CD73 mAb, clone 2B6, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human CD73 protein. Immunogen: Recombinant human CD73 (27-252aa) purified from E Coli

免疫学

CD73 (Ecto-5-nucleotidase, 5-NT) is a membrane-bound glycoprotein that functions to hydrolyze extracellular nucleoside monophosphates into bioactive nucleoside intermediates. This enables the uptake of adenosine, inosine, and guanosine into the cell and their subsequent reconversion into ATP and GTP in the purine salvage pathway. The expression of CD73 on neuronal cells has been described during developmental processes and, on lymphocytes, CD73 serves as a maturation marker, being absent from the surface of both immature B and T cells.

Sern P.C, et al., (2006) Purinergic Signal 2(2):351-360 Linda F.T, et al., (2004) J Exp Med 200(11):1395-1405 Laura A, et al., (1997) J Cell Biol 136(2):421-431

4505467

NP_002517

NM_002526.3

57,949 Da

HIF-1-alpha Transcription Factor Network Pathway (138045); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Nucleotides Pathway (106263); Nicotinate And Nicotinamide Metabolism Pathway (83014); Nicotinate And Nicotinamide Metabolism Pathway (400); Purine Catabolism Pathway (106276); Purine Metabolism Pathway (82944); Purine Metabolism Pathway (106265); Purine Metabolism Pathway (307)

The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]

NT5E: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Homodimer; disulfide-linked. Belongs to the 5 -nucleotidase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Phosphatase (non-protein); Membrane protein, GPI anchor; Membrane protein, integral; EC 3.1.3.5. Chromosomal Location of Human Ortholog: 6q14-q21. Cellular Component: cell surface; membrane; cytoplasm; plasma membrane. Molecular Function: 5 -nucleotidase activity; metal ion binding; nucleotide binding. Biological Process: pyrimidine nucleoside catabolic process; dephosphorylation; pyrimidine base metabolic process; nucleobase, nucleoside and nucleotide metabolic process; negative regulation of inflammatory response; adenosine biosynthetic process; purine nucleotide catabolic process; DNA metabolic process; purine base metabolic process; AMP catabolic process. Disease: Calcification Of Joints And Arteries

0.05毫升

240美元

0.1毫升

315