抗体

多克隆抗体N methylpurine DNA糖苷酶(MPG)

MBS2002169

0.01毫克

135美元

多克隆

兔

未共轭

人类, 小鼠

免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 酶免疫法, 免疫组化-石蜡切片

抗体

多克隆抗体N methylpurine DNA糖苷酶(MPG)

N methylpurine DNA糖苷酶(MPG)

N-methylpurine-DNA glycosylase; DNA-3-methyladenine glycosylase; DNA-3-methyladenine glycosylase; 3-alkyladenine DNA glycosylase; 3-methyladenine DNA glycosidase; proliferation-inducing protein 11; proliferation-inducing protein 16; N-methylpurine-DNA glycosylase, MPG; CRA36.1 (3-methyl-adenine DNA glycosylase); 3' end of the Mid1 gene, localized 68 kb upstream the humanzeta globin gene on 16p; N-methylpurine-DNA glycosylase; 3-alkyladenine DNA glycosylase; 3-methyladenine DNA glycosidase; ADPG; N-methylpurine-DNA glycosylase

MPG

MPG; MPG; AAG; MDG; ADPG; APNG; Mid1; anpg; PIG11; PIG16; CRA36.1; AAG; ANPG; MID1

3 MG_HUMAN

多克隆

IgG

兔

小鼠

271

The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF-LVTGY HEVGQMPAPL SRKIGQKKQR LADSEQQQTP KERLLSTPGL RRSIYFSSPE DHSGRLGPEF FDQPAVTLAR AFLGQVLVRR LADGTELRGR IVETEAYLGP EDEAAHSRGG RQTPRNRGMF MKPGTLYVYL IYGMYFCLNV SSQGAGACVL LRALEPLEGL ETMRQLRNSL RKSTVGRSLK DRELCSGPSK LCQALAIDKS FDQRDLAQDD AVWLEHGPLE SSSPAVVVAA ARIGIGHAGE WTQKPLRFYV QGSPWVSVVD RVAEQ

The antibody is a rabbit polyclonal antibody raised against MPG. It has been selected for its ability to recognize MPG in immunohistochemical staining andwestern blotting.

亲和色谱

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.

200微克/毫升

Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

免疫细胞化学(免疫细胞化学), 免疫组化(免疫组化)-福尔马林/石蜡, 酶联免疫吸附测定(EIA), 免疫印迹(免疫印迹)

Western blotting: 1:100-400. Immunocytochemistry in formalin fixed cells: 1:100-500. Immunohistochemistry in formalin fixed frozen section: 1:100-500. Immunohistochemistry in paraffin section: 1:50-200. Enzyme-linked Immunosorbent Assay: 1:100-200

Immunogen: Recombinant MPG (Leu46~Gln315) expressed in E.coli.

Quality Control: Content: The quality control contains recombinant CRP (Phe17~Pro224) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control.Not used for other purposes. Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.

233968

AAB19537.1

P29372

32,869 Da

Base Excision Repair Pathway (105838); Base Excision Repair Pathway (83043); Base Excision Repair Pathway (451); Base-Excision Repair, AP Site Formation Pathway (105839); Base-free Sugar-phosphate Removal Via The Single-nucleotide Replacement Pathway (105848); Cleavage Of The Damaged Purine Pathway (105842); DNA Repair Pathway (105837); Depurination Pathway (105840); Displacement Of DNA Glycosylase By APE1 Pathway (105849); Recognition And Association Of DNA Glycosylase With Site Containing An Affected Purine Pathway (105841)

MPG: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions. Belongs to the DNA glycosylase MPG family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Hydrolase; Nuclear receptor co-regulator; EC 3.2.2.21. Chromosomal Location of Human Ortholog: 16p13.3. Cellular Component: nucleoplasm. Molecular Function: protein binding; alkylbase DNA N-glycosylase activity; DNA-3-methyladenine glycosylase I activity; damaged DNA binding. Biological Process: DNA dealkylation; base-excision repair, AP site formation; depurination; base-excision repair; DNA repair

0.01毫克

135美元

0.05毫克

245

0.1毫克

325