产品简要
公司名称 :
MyBioSource
产品类型 :
抗体
产品名称 :
多克隆抗体表皮生长因子受体2(EGFR2)
目录 :
MBS2002381
规格 :
0.01毫克
价格 :
105美元
克隆性 :
多克隆
宿主 :
共轭标签 :
未共轭
反应物种 :
人类
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀
更多信息或购买 :
图像
图像 1 :
MyBioSource MBS2002381 图像 1
Western Blot: Sample: Recombinant EGFR2, Human.
图像 2 :
MyBioSource MBS2002381 图像 2
DAB staining on IHC-P. Samples: Human Breast cancer Tissue. Primary Ab: 10 ug/ml Rabbit Anti-Human EGFR2 Antibody . Second Ab: 2 ug/mL HRP-Linked Caprine. Anti-Rabbit IgG Polyclonal Antibody. (Catalog: MBS2086047)
产品信息
目录号 :
MBS2002381
产品类型 :
抗体
产品全称 :
多克隆抗体表皮生长因子受体2(EGFR2)
产品简称 :
[表皮生长因子受体2(EGFR2)]
其他名称 :
[receptor tyrosine-protein kinase erbB-2 isoform b; Receptor tyrosine-protein kinase erbB-2; receptor tyrosine-protein kinase erbB-2; erb-b2 receptor tyrosine kinase 2; Metastatic lymph node gene 19 protein; MLN 19; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2; p185erbB2; CD_antigen: CD340]
产品基因名称 :
[EGFR2]
其他基因名称 :
[ERBB2; ERBB2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu; HER2; MLN19; NEU; NGL; MLN 19]
克隆性 :
多克隆
抗体亚型 :
IgG
宿主 :
纯度 :
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
形式 :
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
浓度 :
0.67毫克/毫升
储存稳定性 :
Avoid repeated freeze/thaw cycles. Store at 4ºC for frequent use. Aliquot and store at -20ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
检测过的应用 :
免疫细胞化学(免疫细胞化学), 免疫组化(免疫组化), 免疫沉淀(免疫沉淀), 免疫印迹(免疫印迹)
应用笔记 :
Western blotting: 0.5-2 ug/mL;1:110-450. Immunohistochemistry: 5-20 ug/mL;1:11-45. Immunocytochemistry: 5-20 ug/mL;1:11-45. Optimal working dilutions must be determined by end user.
image1头 :
免疫印迹(免疫印迹)
image2头 :
免疫组化(免疫组化)
其它信息1 :
Organism Species: Homo sapiens (Human). Source: Polyclonal antibody preparation. Traits: Liquid. Immunogen: Recombinant ErbB2 (Phe376~Gly578) expressed in E.coli
NCBI GI登录号 :
54792098
NCBI登录号 :
NP_001005862.1
NCBI基因登录号 :
NM_001005862.2
UniProt数据库登录号 :
P04626
NCBI信号通路 :
Adaptive Immune System Pathway (366160); Adherens Junction Pathway (83070); Adherens Junction Pathway (481); Alpha6-Beta4 Integrin Signaling Pathway (198807); Axon Guidance Pathway (105688); Bladder Cancer Pathway (83115); Bladder Cancer Pathway (527); Calcium Signaling Pathway (83050); Calcium Signaling Pathway (459); Constitutive PI3K/AKT Signaling In Cancer Pathway (685535)
NCBI总结 :
This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008]
UniProt数据库总结 :
HER2: a proto-oncogenic receptor tyrosine kinase of the EGFR family. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. Not activated by EGF, TGF- alpha and amphiregulin. Amplified in breast cancer. Overexpression induces constitutive activity, and the gene is amplified or overexpressed in up to 30% of breast cancers, correlating with poor survival. The antibody Herceptin is approved for treatment of metastatic breast cancer with HER2 amplification/overexpression. Somatic mutations seen in 4% of lung cancers and also in breast, gastric, ovarian cancer and glioblastoma. One SNP shows predisposition to breast and gastric cancer. Inhibitors: Herceptin, lapatinib, PKI-166, EKB-569, CI-1033. Protein type: EC 2.7.10.1; EGFR family; Kinase, protein; Membrane protein, integral; Oncoprotein; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group. Chromosomal Location of Human Ortholog: 17q12. Cellular Component: apical plasma membrane; basolateral plasma membrane; cytosol; endosome membrane; integral component of membrane; myelin sheath; nucleus; perinuclear region of cytoplasm; plasma membrane; receptor complex. Molecular Function: ATP binding; ErbB-3 class receptor binding; growth factor binding; identical protein binding; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; protein binding; protein C-terminus binding; protein dimerization activity; protein heterodimerization activity; protein phosphatase binding; protein tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity; signal transducer, downstream of receptor, with protein tyrosine kinase activity; transmembrane receptor activity; transmembrane receptor protein tyrosine kinase activity. Biological Process: cell proliferation; cell surface receptor signaling pathway; cellular response to epidermal growth factor stimulus; cellular response to growth factor stimulus; enzyme linked receptor protein signaling pathway; ERBB2 signaling pathway; heart development; MAPK cascade; motor axon guidance; myelination; negative regulation of ERBB signaling pathway; negative regulation of immature T cell proliferation in the thymus; neuromuscular junction development; oligodendrocyte differentiation; peptidyl-tyrosine phosphorylation; peripheral nervous system development; phosphatidylinositol phosphorylation; phosphoinositide 3-kinase cascade; positive regulation of cell adhesion; positive regulation of cell growth; positive regulation of epithelial cell proliferation; positive regulation of GTPase activity; positive regulation of MAP kinase activity; positive regulation of protein kinase B signaling; positive regulation of protein phosphorylation; positive regulation of protein targeting to membrane; positive regulation of transcription from RNA polymerase I promoter; positive regulation of transcription from RNA polymerase III promoter; positive regulation of translation; protein amino acid phosphorylation; protein autophosphorylation; regulation of angiogenesis; regulation of cell motility; regulation of ERK1 and ERK2 cascade; regulation of microtubule-based process; regulation of transcription from RNA polymerase II promoter; signal transduction; transcription, DNA-dependent; transmembrane receptor protein tyrosine kinase signaling pathway; wound healing. Disease: Gastric Cancer; Glioma Susceptibility 1; Lung Cancer
尺寸1 :
0.01毫克
价格1 :
105美元
尺寸2 :
0.02毫克
价格2 :
125
size3 :
0.05毫克
价格3 :
170
size4 :
0.1毫克
price4 :
220
size5 :
0.2毫克
price5 :
320
size6 :
1毫克
price6 :
760
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。