产品简要
公司名称 :
MyBioSource
产品类型 :
抗体
产品名称 :
CD3E抗体
目录 :
MBS200286
规格 :
0.05毫升
价格 :
240美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
[AT3H3]
反应物种 :
人类
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 流式细胞仪, 流式细胞仪, 酶免疫法
更多信息或购买 :
图像
图像 1 :
Fig. 1: The extract of Jurkat (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CD3E antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Fig. 2: The Human Recombinant protein CD3E (200, 100, 50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CD3E antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
图像 2 :
ICC/IF analysis of CD3E in Jurkat cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CD3E antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
图像 3 :
Flow cytometry analysis of CD3E in Jurkat cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
产品信息
目录号 :
MBS200286
产品类型 :
抗体
产品全称 :
CD3E抗体
产品简称 :
[CD3E]
产品名称同义词 :
[T-cell surface glycoprotein CD3 epsilon chain; T3E; TCRE]
其他名称 :
[T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; CD3e molecule; T-cell surface antigen T3/Leu-4 epsilon chain; CD_antigen: CD3e]
产品基因名称 :
[CD3E]
其他基因名称 :
[CD3E;CD3E;T3E;TCRE;IMD18;T3E]
克隆性 :
单克隆
抗体亚型 :
IgG1, k
克隆 :
[AT3H3]
宿主 :
小鼠
反应物种 :
人类
序列长度 :
207
纯度 :
By protein-A affinity chromatography
形式 :
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
浓度 :
1毫克/毫升
储存稳定性 :
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
检测过的应用 :
酶联免疫吸附测定(EIA), 免疫印迹(免疫印迹), 流式细胞仪(FC/流式细胞仪), 免疫细胞化学(免疫细胞化学), 免疫荧光(IF)
应用笔记 :
Recommended starting dilution is 1:1000.
image1头 :
免疫印迹(免疫印迹)
image2头 :
免疫荧光(IF)
image3头 :
流式细胞仪(FC)
其它信息1 :
Antigen Species: Human. Immunogen: Recombinant human CD3E (23-126aa) purified from E. coli
其他信息2 :
Clone Note: Anti-human CD3E mAb, clone AT3H3, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human CD3E protein.
产品种类 :
免疫学
产品描述 :
The T cell antigen receptor (TCR) recognizes foreign antigens and translates such recognition events into intracellular signals that elicit a change in the cell from a dormant to an activated state. Much of this signaling process can be attributed to a multi subunit complex of proteins that associates directly with the TCR. This complex has been designated CD3 (cluster of differentiation 3). It is composed of five invariant polypeptide chains that associate to form three dimers. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women.
产品引用 :
Gold DP., et al. (1986) Nature 321 (6068): 431-434; Soudais C., et al. (1993) Nat Genet 3 (1):77-81.
NCBI GI登录号 :
4502671
NCBI登录号 :
NP_000724
NCBI基因登录号 :
NM_000733.3
UniProt数据库登录号 :
P07766
NCBI信号通路 :
Adaptive Immune System Pathway (1269171); CXCR4-mediated Signaling Events Pathway (137910); Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795); Costimulation By The CD28 Family Pathway (1269177); Downstream TCR Signaling Pathway (1269176); Downstream Signaling In Naive CD8+ T Cells Pathway (138018); Generation Of Second Messenger Molecules Pathway (1269175); HTLV-I Infection Pathway (373901); HTLV-I Infection Pathway (373889)
NCBI总结 :
The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]
UniProt数据库总结 :
Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell developement. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed:10384095, PubMed:26507128).
尺寸1 :
0.05毫升
价格1 :
240美元
尺寸2 :
0.1毫升
价格2 :
315
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华，公司愿景是“生物研究试剂的源头”，现在位于圣地亚哥市。