产品简要
公司名称 :
MyBioSource
产品类型 :
蛋白
产品名称 :
重组层粘连蛋白甲3(LAMa3)
目录 :
MBS2010860
规格 :
0.01毫克
价格 :
140美元
更多信息或购买 :
图像
图像 1 :
MyBioSource MBS2010860 图像 1
图像 2 :
MyBioSource MBS2010860 图像 2
Figure. SDS-PAGE
产品信息
目录号 :
MBS2010860
产品类型 :
重组蛋白
产品全称 :
重组层粘连蛋白甲3(LAMa3)
产品简称 :
[层粘连蛋白甲3(LAMa3)]
其他名称 :
[laminin subunit alpha-3 isoform 2; Laminin subunit alpha-3; laminin subunit alpha-3; BM600 150kD subunit; nicein 150kD subunit; nicein subunit alpha; kalinin 165kD subunit; kalinin subunit alpha; epiligrin subunit alpha; laminin-5 alpha 3 chain; laminin-5 subunit alpha; laminin-6 subunit alpha; laminin-7 subunit alpha; epiligrin 170 kda subunit; epiligrin alpha 3 subunit; laminin, alpha 3 (nicein (150kD), kalinin (165kD), BM600 (150kD), epilegrin); laminin, alpha 3; Epiligrin 170 kDa subunit; E170; Epiligrin subunit alpha; Kalinin subunit alpha; Laminin-5 subunit alpha; Laminin-6 subunit alpha; Laminin-7 subunit alpha; Nicein subunit alpha]
产品基因名称 :
[LAMa3]
其他基因名称 :
[LAMA3;LAMA3;E170;LOCS;BM600;LAMNA;lama3a;LAMNA;E170]
UniProt数据库进入名 :
LAMA3_HUMAN
宿主 :
大肠杆菌
纯度 :
> 90%
形式 :
20mM三羟甲基氨基甲烷, 150mM氯化钠, pH8.0, 含0.01% SKL, 5% 海藻糖
浓度 :
Original Concentration: 50 ug/mL
储存稳定性 :
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8ºC for one month. Aliquot and store at -80ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
检测过的应用 :
Positive Control; Immunogen; SDS-PAGE; Western blot (WB). (May be suitable for use in other assays to be determined by the end user.)
应用笔记 :
Identification: Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/mL. Do not votex.
image1头 :
序列信息
image2头 :
十二烷基磺酸钠-PAGE
其它信息1 :
Organism Species: Homo sapiens (Human). Source: Prokaryotic expression. Residues: Gln47~Leu296. Tags: N-terminal His Tag. Subcellular Location: Secreted, Extracellular matrix. Traits: Freeze-dried powder
其他信息2 :
Predicted isoelectric point: 5.9. Predicted Molecular Mass: 28.3kDa. Accurate Molecular Mass: 33kDa as determined by SDS-PAGE reducing conditions. Phenomenon explanation: The possible reasons that the actual band size differs from the predicted are as follows:. 1.Splice variants: Alternative splicing may create different sized proteins from the same gene. 2. Relative charge: The composition of amino acids may affects the charge of the protein. 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc. 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form. 5. Polymerization of the target protein: Dimerization, multimerization etc. Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
NCBI GI登录号 :
38045908
NCBI登录号 :
NP_000218.2
NCBI基因登录号 :
NM_000227.3
UniProt数据库登录号 :
Q16787
NCBI信号通路 :
Alpha6-Beta4 Integrin Signaling Pathway (198807); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Anchoring Fibril Formation Pathway (730307); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (730306); Cell Junction Organization Pathway (160966); Cell-Cell Communication Pathway (477132); Collagen Formation Pathway (645288); Degradation Of The Extracellular Matrix Pathway (576263); ECM Proteoglycans Pathway (833812)
NCBI总结 :
The protein encoded by this gene belongs to the laminin family of secreted molecules. Laminins are heterotrimeric molecules that consist of alpha, beta, and gamma subunits that assemble through a coiled-coil domain. Laminins are essential for formation and function of the basement membrane and have additional functions in regulating cell migration and mechanical signal transduction. This gene encodes an alpha subunit and is responsive to several epithelial-mesenchymal regulators including keratinocyte growth factor, epidermal growth factor and insulin-like growth factor. Mutations in this gene have been identified as the cause of Herlitz type junctional epidermolysis bullosa and laryngoonychocutaneous syndrome. Alternative splicing and alternative promoter usage result in multiple transcript variants. [provided by RefSeq, Dec 2014]
UniProt数据库总结 :
LAMA3: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Defects in LAMA3 are a cause of epidermolysis bullosa junctional Herlitz type (H-JEB); also known as junctional epidermolysis bullosa Herlitz-Pearson type. JEB defines a group of blistering skin diseases characterized by tissue separation which occurs within the dermo-epidermal basement membrane. H-JEB is a severe, infantile and lethal form. Death occurs usually within the first six months of life. Occasionally, children survive to teens. H-JEB is marked by bullous lesions at birth and extensive denudation of skin and mucous membranes that may be hemorrhagic. Defects in LAMA3 are the cause of laryngoonychocutaneous syndrome (LOCS). LOCS is an autosomal recessive epithelial disorder confined to the Punjabi Muslim population. The condition is characterized by cutaneous erosions, nail dystrophy and exuberant vascular granulation tissue in certain epithelia, especially conjunctiva and larynx. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 18q11.2. Cellular Component: laminin-5 complex; laminin-1 complex; extracellular region; basement membrane. Molecular Function: structural molecule activity; receptor binding. Biological Process: regulation of cell adhesion; extracellular matrix disassembly; hemidesmosome assembly; epidermis development; extracellular matrix organization and biogenesis; regulation of embryonic development; cell adhesion; regulation of cell migration. Disease: Epidermolysis Bullosa, Junctional, Non-herlitz Type; Laryngoonychocutaneous Syndrome; Epidermolysis Bullosa, Junctional, Herlitz Type
尺寸1 :
0.01毫克
价格1 :
140美元
尺寸2 :
0.05毫克
价格2 :
255
size3 :
0.1毫克
价格3 :
390
size4 :
0.2毫克
price4 :
480
size5 :
0.5毫克
price5 :
935
size6 :
1毫克
price6 :
1380
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。