蛋白

Recombinant Nitric Oxide Synthase 1, Neuronal (NOS1)

MBS2012434

0.01毫克

145美元

重组蛋白

Recombinant Nitric Oxide Synthase 1, Neuronal (NOS1)

[Nitric Oxide Synthase 1, Neuronal (NOS1)]

[nitric oxide synthase, brain; Nitric oxide synthase, brain; nitric oxide synthase, brain; N-NOS; NC-NOS; NOS type I; neuronal NOS; constitutive NOS; neuronal nitric oxide synthase; peptidyl-cysteine S-nitrosylase NOS1; nitric oxide synthase 1, neuronal; Constitutive NOS; NC-NOS; NOS type I; Neuronal NOS; N-NOS; nNOS; Peptidyl-cysteine S-nitrosylase NOS1; bNOS]

[NOS1]

[Nos1; Nos1; NO; bNOS; nNOS; NOS-I; Nos-1; 2310005C01Rik; N-NOS; nNOS]

NOS1_MOUSE

大肠杆菌

1429

The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSGSEF-FHQEMLNYRL TPSFEYQPDP WNTHVWKGTN GTPTKRRAIG FKKLAEAVKF SAKLMGQAMA KRVKATILYA TETGKSQAYA KTLCEIFKHA FDAKAMSMEE YDIVHLEHEA LVLVVTSTFG NGDPPENGEK FGCALM

>92%

20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), CoIP, ReporterAssays, Purification, Amine Reactive Labeling.

序列信息

SDS-Page

Organism: Mus musculus (Mouse). Expression System: Prokaryotic expression. Residues: Phe691~Met826 with N-terminal His-Tag. Tissue Specificity: Brain. Subcellular Location: Cell membrane, sarcolemma; Peripheral membrane protein. Cell projection, dendritic spine. Traits: Freeze-dried powder. Predicted isoelectric point: 7.9. Predicted Molecular Mass: 16.8kDa. Accurate Molecular Mass: 18kDa as determined by SDS-PAGE reducing conditions

Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

6724321

NP_032738.1

NM_008712.2

Q9Z0J4

Alzheimer's Disease Pathway (83294); Alzheimer's Disease Pathway (509); Amyotrophic Lateral Sclerosis (ALS) Pathway (83296); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Arginine And Proline Metabolism Pathway (83157); Arginine And Proline Metabolism Pathway (323); Calcium Signaling Pathway (83247); Calcium Signaling Pathway (459); Circadian Entrainment Pathway (698783); Circadian Entrainment Pathway (699872)

nNOS: nitric oxide synthase 1, neuronal form. Produces nitric oxide which is a messenger molecule with diverse functions and displays many properties of a neurotransmitter in the brain and peripheral nervous system. May be an effector enzyme for the dystrophin complex. Four alternatively spliced isoforms have been described. Protein type: EC 1.14.13.39; Amino Acid Metabolism - arginine and proline; Oxidoreductase. Cellular Component: sarcoplasmic reticulum membrane; nuclear membrane; protein complex; photoreceptor inner segment; mitochondrion; sarcoplasmic reticulum; postsynaptic density; T-tubule; dendrite; caveola; vesicle membrane; cytosol; Z disc; lipid raft; azurophil granule; mitochondrial outer membrane; cell projection; cytoskeleton; membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; synapse; nucleus; sarcolemma. Molecular Function: protein homodimerization activity; FAD binding; nitric-oxide synthase activity; metal ion binding; oxidoreductase activity; cadmium ion binding; ATPase binding; calmodulin binding; protein binding; enzyme binding; sodium channel regulator activity; NADPH-hemoprotein reductase activity; FMN binding; iron ion binding; heme binding; NADP binding. Biological Process: negative regulation of serotonin uptake; response to peptide hormone stimulus; striated muscle contraction; negative regulation of heart contraction; positive regulation of transcription, DNA-dependent; negative regulation of potassium ion transport; negative regulation of peptidyl-serine phosphorylation; positive regulation of vasodilation; regulation of heart contraction; negative regulation of insulin secretion; negative regulation of cell proliferation; behavioral response to cocaine; negative regulation of hydrolase activity; negative regulation of blood pressure; nitric oxide biosynthetic process; negative regulation of calcium ion transport; positive regulation of guanylate cyclase activity; regulation of sodium ion transport; negative regulation of vasoconstriction; response to estrogen stimulus; response to heat; reduction of cytosolic calcium ion concentration; exogenous drug catabolic process; peptidyl-cysteine S-nitrosylation; arginine catabolic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of histone acetylation; regulation of sensory perception of pain; nitric oxide mediated signal transduction; multicellular organismal response to stress; negative regulation of apoptosis

0.01毫克

145美元

0.05毫克

280

0.1毫克

430

0.2毫克

530

0.5毫克

1020

1毫克

1520