抗体

异硫氰酸荧光素链接抗体Toll样受体4(Toll样受体4)

MBS2012886

0.1毫克

255美元

多克隆

兔

异硫氰酸荧光素

人类, 大鼠

免疫印迹, 免疫组化, 免疫细胞化学

抗体

异硫氰酸荧光素链接抗体Toll样受体4(Toll样受体4)

[Toll样受体4(Toll样受体4)]

[toll-like receptor 4 isoform A; Toll-like receptor 4; toll-like receptor 4; hToll; homolog of Drosophila toll; toll-like receptor 4; hToll]

[Toll样受体4]

[Toll样受体4;Toll样受体4;TOLL;CD284;TLR-4;ARMD10]

TLR4_HUMAN

多克隆

大鼠IgG

兔

大鼠

Antigen: The target protein is fused with a His-tag and its sequence is listed below. MGHHHHHHSGSEF-KNL KSPQKVDEVDI IDDNFILRWN RSDESCGNVT FSFDYQKTGM DNWIKLSGCQ NITSTKCNFSSLKLNBYEEI KLRIRAEKEN TSSWYEVDSF TPFFRKAQIGP PEVHLEAEDK AIVIHISPGT KDSVMWALDG LSFTYSLVIWKNSSGVEERI ENIYSRHKIY KLSPETTYCL KVKALLLTSW KIGVYSPVHC IKTTVEN

The antibody is a rabbit polyclonal antibody raised against TLR4.This antibody has been selected for its ability to recognize TLR4 in immunohistochemical staining and western blotting.

Antigen-specific Affinity Chromatography.

Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

0.36毫克/毫升

Store at 4°C for frequent use. Stored at -20°C to -80°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze- thaw cycles.

免疫印迹;免疫组化;免疫细胞化学;IF

Western blotting: 0.5-2 Ug/mL;1:180-700 . Immunohistochemistry: 5-20 Ug/mL;1:18-70. Immunocytochemistry: 5-20 Ug/mL;1:18-70 . Optimal working dilutions must be determined by end user.

Immunogen: Recombinant TLR4 (His49~Gln247) expressed in E.coli.

异硫氰酸荧光素抗体

19924149

NP_612564.1

NM_138554.4

O00206

Activated TLR4 Signalling Pathway (106400); Activation Of IRF3/IRF7 Mediated By TBK1/IKK Epsilon Pathway (187088); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795); Corticotropin-releasing Hormone Pathway (920957); HIF-1 Signaling Pathway (695200); Hepatitis B Pathway (694606); IKK Complex Recruitment Mediated By RIP1 Pathway (187090)

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. Mutations in this gene have been associated with differences in LPS responsiveness. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

TLR4: Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS- independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific. Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, LY96 and TLR4. Binding to bacterial LPS leads to homodimerization. Interacts with LY96 via the extracellular domain. Interacts with MYD88 and TIRAP via their respective TIR domains. Interacts with NOX4. Interacts with CNPY3. Interacts with HSP90B1. The interaction with both CNPY3 and HSP90B1 is required for proper folding in the endoplasmic reticulum. Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells. Belongs to the Toll-like receptor family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Receptor, misc.; Membrane protein, integral. Chromosomal Location of Human Ortholog: 9q33.1. Cellular Component: perinuclear region of cytoplasm; integral to plasma membrane; cytoplasm; plasma membrane; lipopolysaccharide receptor complex; endosome membrane; external side of plasma membrane. Molecular Function: protein binding; transmembrane receptor activity; lipopolysaccharide binding; lipopolysaccharide receptor activity; receptor activity. Biological Process: I-kappaB kinase/NF-kappaB cascade; positive regulation of nitric oxide biosynthetic process; activation of MAPK activity; response to lipopolysaccharide; positive regulation of NF-kappaB import into nucleus; toll-like receptor 3 signaling pathway; positive regulation of interleukin-10 production; activation of NF-kappaB transcription factor; negative regulation of interleukin-6 production; positive regulation of B cell proliferation; positive regulation of interferon-beta production; toll-like receptor 4 signaling pathway; positive regulation of interferon-alpha production; T-helper 1 type immune response; production of nitric oxide during acute inflammatory response; positive regulation of interleukin-8 biosynthetic process; positive regulation of interleukin-6 production; positive regulation of tumor necrosis factor production; positive regulation of chemokine production; toll-like receptor 2 signaling pathway; macrophage activation; negative regulation of interleukin-23 production; detection of lipopolysaccharide; defense response to bacterium; positive regulation of transcription from RNA polymerase II promoter; I-kappaB phosphorylation; positive regulation of nitric-oxide synthase biosynthetic process; negative regulation of osteoclast differentiation; regulation of cytokine secretion; positive regulation of interleukin-12 production; positive regulation of JNK cascade; defense response to Gram-negative bacterium; positive regulation of interleukin-8 production; positive regulation of MHC class II biosynthetic process; negative regulation of interferon-gamma production; interferon-gamma production; lipopolysaccharide-mediated signaling pathway; B cell proliferation during immune response; positive regulation of tumor necrosis factor biosynthetic process; detection of fungus; MyD88-independent toll-like receptor signaling pathway; negative regulation of tumor necrosis factor production; MyD88-dependent toll-like receptor signaling pathway; positive regulation of interferon-gamma production; negative regulation of interleukin-17 production; positive regulation of interleukin-12 biosynthetic process; positive regulation of interferon-beta biosynthetic process; toll-like receptor signaling pathway; innate immune response; immune response; positive regulation of interleukin-1 production; positive regulation of inflammatory response. Disease: Macular Degeneration, Age-related, 10

0.1毫克

255美元

0.2毫克

390

1毫克

935