抗体

Polyclonal Antibody to Fc Fragment Of IgG Low Affinity IIIb Receptor (FcgR3B)

MBS2013505

0.01毫克

130美元

多克隆

兔

未共轭

免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 酶免疫法, 免疫组化-石蜡切片

抗体

Polyclonal Antibody to Fc Fragment Of IgG Low Affinity IIIb Receptor (FcgR3B)

Fc Fragment Of IgG Low Affinity IIIb Receptor (FcgR3B)

Fc fragment of IgG, low affinity IIIb, receptor (CD16b); Low affinity immunoglobulin gamma Fc region receptor III-B; low affinity immunoglobulin gamma Fc region receptor III-B; fc-gamma RIII; fc-gamma RIIIb; fc-gamma RIII-beta; igG Fc receptor III-1; Fc-gamma receptor IIIb (CD 16); Fc fragment of IgG, low affinity IIIb, receptor for (CD16); Fc fragment of IgG, low affinity IIIb, receptor (CD16b); Fc-gamma RIII-beta; Fc-gamma RIII; Fc-gamma RIIIb; FcRIII; FcRIIIb; FcR-10; IgG Fc receptor III-1

FcgR3B

FCGR3B;FCGR3B;CD16;FCG3;CD16b;FCGR3;FCR-10;FCRIII;FCRIIIb;CD16B;FCG3;FCGR3;IGFR3;Fcγ受体Ⅲ;Fc丙RIIIb;FcRIII;FcRIIIb

FCG3B_HUMAN

多克隆

IgG

兔

人类

233

The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- PKAVVFL EPQWYSVLEK DSVTLKCQGA YSPEDNSTQW FHNESLISSQ ASSYFIDAAT VNDSGEYRCQ TNLSTLSDPV QLEVHIGWLL LQAPRWVFKE EDPIHLRCHS WKNTALHKVT YLQNGKDRKY FHHNSDFHIP

The antibody is a rabbit polyclonal antibody raised against FcgR3B. It has been selected for its ability to recognize FcgR3B in immunohistochemical staining andwestern blotting.

亲和色谱

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.

200微克/毫升

Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

免疫细胞化学(免疫细胞化学), 免疫组化(免疫组化)-福尔马林/石蜡, 酶联免疫吸附测定(EIA), 免疫印迹(免疫印迹)

Western blotting: 1:50-400. Immunocytochemistry in formalin fixed cells: 1:50-500. Immunohistochemistry in formalin fixed frozen section: 1:50-500. Immunohistochemistry in paraffin section: 1:10-100. Enzyme-linked Immunosorbent Assay: 1:100-200. Optimal working dilutions must be determined by end user.

Immunogen: Recombinant FcgR3B (Pro24~Ser203) expressed in E.coli. Accession No.: RPB271Hu01

Quality Control: Content: The quality control contains recombinant CRP (Phe17~Pro224) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control.Not used for other purposes. Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.

118764253

AAI28563.1

O75015

26,216 Da

Leishmaniasis Pathway (144181); Leishmaniasis Pathway (144028); Natural Killer Cell Mediated Cytotoxicity Pathway (83079); Natural Killer Cell Mediated Cytotoxicity Pathway (490); Osteoclast Differentiation Pathway (193147); Osteoclast Differentiation Pathway (193096); Phagosome Pathway (153910); Phagosome Pathway (153859); Staphylococcus Aureus Infection Pathway (172846); Staphylococcus Aureus Infection Pathway (171867)

The protein encoded by this gene is a low affinity receptor for the Fc region of gamma immunoglobulins (IgG). The encoded protein acts as a monomer and can bind either monomeric or aggregated IgG. This gene may function to capture immune complexes in the peripheral circulation. Several transcript variants encoding different isoforms have been found for this gene. A highly-similar gene encoding a related protein is also found on chromosome 1. [provided by RefSeq, Aug 2012]

Function: Receptor for the Fc region of immunoglobulins gamma. Low affinity receptor. Binds complexed or aggregated IgG and also monomeric IgG. Contrary to III-A, is not capable to mediate antibody-dependent cytotoxicity and phagocytosis. May serve as a trap for immune complexes in the peripheral circulation which does not activate neutrophils. Subunit structure: Monomer. Interacts with INPP5D/SHIP1 . By similarity. Subcellular location: Cell membrane; Lipid-anchor GPI-anchor. Secreted. Note: Secreted after cleavage. Tissue specificity: Expressed specifically by polymorphonuclear leukocytes (neutrophils). Also expressed by stimulated eosinophils. Post-translational modification: Glycosylated. Glycosylation plays an inhibitory role in the interaction with IgG3.The soluble form is produced by a proteolytic cleavage. Polymorphism: There are three allelic forms of FCGR3B: FCGR3B*01 (NA-1), FCGR3B*02 (HNA-1b, NA-2) (shown here) and SH. FCGR3B*01 and FCGR3B*02 are detectable with antibodies against the biallelic neutrophil-specific antigen system NA. The more active FCGR3B*01 allele has been associated with severe renal disease in certain systemic vasculitides. Miscellaneous: Encoded by one of two nearly indentical genes: FCGR3A and FCGR3B (Shown here) which are expressed in a tissue-specific manner. The 'Phe-203' in FCGR3A determines the transmembrane domains whereas the Ser-203 in FCGR3B determines the GPI-anchoring. Sequence similarities: Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

0.01毫克

130美元

0.05毫克

225

0.1毫克

295