ELISA/assay

谷草转氨酶(AST)酶联免疫吸附试剂盒

MBS2019541

24-Strip-Wells

270美元

酶联免疫吸附试剂盒

谷草转氨酶(AST)酶联免疫吸附试剂盒

[谷草转氨酶(AST)]

[cCAT; AAT; ASAT; SGOT; GOT1; Cysteine transaminase, cytoplasmic; Transaminase A; Aspartate Transaminase 1,Cytoplasmic; Glutamic-Oxaloacetic Transaminase 1,Soluble]

[aspartate aminotransferase; Aspartate aminotransferase, chloroplastic; aspartate aminotransferase; Transaminase A]

[AST]

[ASP5;ASP5;AAT3;ASP5;ASPARTATE AMINOTRANSFERASE;谷草转氨酶5;ASPARTATE AMINOTRANSFERASE DEFICIENT 3;ATAAT1;F11C18.15;]

AAT5_ARATH

大鼠

448

This assay has high sensitivity and excellent specificity for detection of AST. No significant cross-reactivity or interference between AST and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Samples: Mouse Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.312-20ng/mL. Sensitivity: 0.121ng/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level AST were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level AST were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Enzyme Tumor immunity; Infection immunity; Hepatology

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of AST in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to AST. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to AST. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain AST, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of AST in the samples is then determined by comparing the O.D. of the samples to the standard curve.

334187077

NP_001190885.1

NM_001203956.1

P46248

49,831 Da

2-Oxocarboxylic Acid Metabolism Pathway (714629); 2-Oxocarboxylic Acid Metabolism Pathway (717400); Alanine, Aspartate And Glutamate Metabolism Pathway (100103); Alanine, Aspartate And Glutamate Metabolism Pathway (100063); Amino Acid Synthesis And Interconversion (transamination) Pathway (1118420); Arginine And Proline Metabolism Pathway (4017); Arginine And Proline Metabolism Pathway (323); Biosynthesis Of Amino Acids Pathway (790118); Biosynthesis Of Amino Acids Pathway (795174); Biosynthesis Of Secondary Metabolites Pathway (148695)

encodes a plastid-localized aspartate aminotransferase

Amino acid aminotransferase important for the metabolism of amino acids and Krebs-cycle related organic acids. No activity with D-Asp or D-Ala as amino donors. In plants, it is involved in nitrogen metabolism and in aspects of carbon and energy metabolism.

24-Strip-Wells

270美元

48-Strip-Wells

505

96-Strip-Wells

685

5x96-Strip-Wells

2775

10x96-Strip-Wells

5035