产品简要
公司名称 :
MyBioSource
产品类型 :
ELISA/assay
产品名称 :
电压依赖性阴离子通道蛋白1(VDAC1)酶联免疫吸附试剂盒
目录 :
MBS2020306
规格 :
48-Strip-Wells
价格 :
565美元
更多信息或购买 :
产品信息
目录号 :
MBS2020306
产品类型 :
酶联免疫吸附试剂盒
产品全称 :
电压依赖性阴离子通道蛋白1(VDAC1)酶联免疫吸附试剂盒
产品简称 :
电压依赖性阴离子通道蛋白1(VDAC1)
产品名称同义词 :
PORIN-31-HL; Porin 31HM; Plasmalemmal porin; Outer mitochondrial membrane protein porin 1; Voltage-dependent anion-selective channel protein 1
其他名称 :
voltage dependent anion channel 1; Mitochondrial outer membrane protein porin 1; voltage dependent anion channel 1; Voltage-dependent anion-selective channel protein 1; AtVDAC1; VDAC-1
产品基因名称 :
VDAC1
其他基因名称 :
VDAC1;VDAC1;5076;ARABIDOPSIS THALIANA VOLTAGE DEPENDENT阴离子CHANNEL 1;ATVDAC1;T22N4.9;T22N4_9;VDAC1;电压依赖性阴离子通道1;AtVDAC1;VDAC-1
UniProt数据库进入名 :
VDAC1_ARATH
反应物种 :
人类
序列长度 :
276
特异性 :
This assay has high sensitivity and excellent specificity for detection of Voltage Dependent Anion Channel Protein 1 (VDAC1). No significant cross-reactivity or interference between Voltage Dependent Anion Channel Protein 1 (VDAC1) and analogues was observed.
储存稳定性 :
For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
其它信息1 :
Samples: Tissue homogenates, Cell lysates and other biological fluids. Assay Type: Sandwich. Detection Range: 31.25-2000pg/mL. Sensitivity: Typically less than 12.5pg/mL.
其他信息2 :
Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Voltage Dependent Anion Channel Protein 1 (VDAC1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Voltage Dependent Anion Channel Protein 1 (VDAC1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.
产品描述 :
Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Voltage Dependent Anion Channel Protein 1 (VDAC1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Voltage Dependent Anion Channel Protein 1 (VDAC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Voltage Dependent Anion Channel Protein 1 (VDAC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Voltage Dependent Anion Channel Protein 1 (VDAC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI GI登录号 :
15232074
NCBI登录号 :
NP_186777.1
NCBI基因登录号 :
NM_110994.3
UniProt数据库登录号 :
Q9SRH5
NCBI分子量 :
29,425 Da
NCBI总结 :
Encodes a voltage-dependent anion channel (VDAC: AT3G01280/VDAC1, AT5G67500/VDAC2, AT5G15090/VDAC3, AT5G57490/VDAC4, AT5G15090/VDAC5). VDACs are reported to be porin-type, beta-barrel diffusion pores. They are prominently localized in the outer mitochondrial membrane and are involved in metabolite exchange between the organelle and the cytosol.
尺寸1 :
48-Strip-Wells
价格1 :
565美元
尺寸2 :
96-Strip-Wells
价格2 :
765
size3 :
5x96-Strip-Wells
价格3 :
2975
size4 :
10x96-Strip-Wells
price4 :
5340
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。