产品简要
公司名称 :
MyBioSource
产品类型 :
ELISA/assay
产品名称 :
Perilipin 4 (PLIN4) ELISA Kit
目录 :
MBS2020408
规格 :
96 Strip Wells
价格 :
780美元
更多信息或购买 :
产品信息
目录号 :
MBS2020408
产品类型 :
酶联免疫吸附试剂盒
产品全称 :
Perilipin 4 (PLIN4) ELISA Kit
产品简称 :
Perilipin 4 (PLIN4)
产品名称同义词 :
脂肪细胞 蛋白S3-12
其他名称 :
perilipin-4; Perilipin-4; perilipin-4; perilipin 4; Adipocyte protein S3-12
产品基因名称 :
PLIN4
其他基因名称 :
PLIN4;PLIN4;S3-12;KIAA1881;KIAA1881
UniProt数据库进入名 :
PLIN4_HUMAN
反应物种 :
人类
序列长度 :
1357
特异性 :
This assay has high sensitivity and excellent specificity for detection of Perilipin 4 (PLIN4). No significant cross-reactivity or interference between Perilipin 4 (PLIN4) and analogues was observed.
储存稳定性 :
For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
其它信息1 :
Sample Types: Tissue homogenates and other biological fluids. Detection Range: 0.313-20ng/mL. Sensitivity: The minimum detectable dose of this kit is typically less than 0.117ng/mL.
其他信息2 :
Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Perilipin 4 (PLIN4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Perilipin 4 (PLIN4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.
产品描述 :
Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Perilipin 4 (PLIN4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Perilipin 4 (PLIN4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Perilipin 4 (PLIN4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Perilipin 4 (PLIN4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI GI登录号 :
122937195
NCBI登录号 :
NP_001073869.1
NCBI基因登录号 :
NM_001080400.1
UniProt数据库登录号 :
Q96Q06
NCBI分子量 :
140,675 Da
NCBI总结 :
Members of the perilipin family, such as PLIN4, coat intracellular lipid storage droplets (Wolins et al., 2003 [PubMed 12840023]).[supplied by OMIM, Feb 2010]
规格 :
96 Strip Wells
价格 :
780美元
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。