ELISA/assay

谷胱甘肽S转移甲5(GSTa5)酶联免疫吸附试剂盒

MBS2020611

96 Strip Wells

745美元

酶联免疫吸附试剂盒

谷胱甘肽S转移甲5(GSTa5)酶联免疫吸附试剂盒

谷胱甘肽S转移甲5(GSTa5)

GST-a5; Glutathione S-Transferase A5; GST class-alpha member 5

glutathione S-transferase alpha-5 isoform 1; Glutathione S-transferase alpha-5; glutathione S-transferase alpha-5; glutathione S-transferase alpha 3; GST A5-5; Glutathione S-transferase Yc-2; GST Yc2

GSTa5

Gsta3;Gsta5;Yc2;Gsta5;Gstyc2;谷胱甘肽S-转移酶Yc2

GSTA5_RAT

人类

221

This assay has high sensitivity and excellent specificity for detection of Glutathione S Transferase Alpha 5 (GSTa5). No significant cross-reactivity or interference between Glutathione S Transferase Alpha 5 (GSTa5) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Sample Types: Serum, plasma and other biological fluids. Detection Range: 0.625-40ng/mL. Sensitivity: The minimum detectable dose of this kit is typically less than 0.235ng/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutathione S Transferase Alpha 5 (GSTa5) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutathione S Transferase Alpha 5 (GSTa5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutathione S Transferase Alpha 5 (GSTa5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutathione S Transferase Alpha 5 (GSTa5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutathione S Transferase Alpha 5 (GSTa5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Glutathione S Transferase Alpha 5 (GSTa5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

229092283

NP_001153211.1

NM_001159739.2

P46418

25,347 Da

Biological Oxidations Pathway 1121111!!Chemical Carcinogenesis Pathway 673233!!Chemical Carcinogenesis Pathway 673237!!Defective AHCY Causes Hypermethioninemia With S-adenosylhomocysteine Hydrolase Deficiency (HMAHCHD) Pathway 1121991!!Defective GCLC Causes Hemolytic Anemia Due To Gamma-glutamylcysteine Synthetase Deficiency (HAGGSD) Pathway 1122010!!Defective GGT1 Causes Glutathionuria (GLUTH) Pathway 1122011!!Defective GSS Causes Glutathione Synthetase Deficiency (GSS Deficiency) Pathway 1122012!!Defective MAT1A Causes Methionine Adenosyltransferase Deficiency (MATD) Pathway 1122015!!Defective OPLAH Causes 5-oxoprolinase Deficiency (OPLAHD) Pathway 1122013!!Defective SLC35D1 Causes Schneckenbecken Dysplasia (SCHBCKD) Pathway 1122017

96 Strip Wells

745美元