ELISA/assay

血管内皮生长因子121(VEGF121)酶联免疫吸附试剂盒

MBS2021334

96 Strip Wells

800美元

酶联免疫吸附试剂盒

血管内皮生长因子121(VEGF121)酶联免疫吸附试剂盒

血管内皮生长因子121(VEGF121)

vascular endothelial growth factor isoform 121; Vascular endothelial growth factor A; vascular endothelial growth factor A; vascular endothelial growth factor A; Vascular permeability factor; VPF

VEGF121

血管内皮生长因子A;血管内皮生长因子A;VPF;血管内皮生长因子;MVCD1;血管内皮生长因子;血管内皮生长因子-A;VPF

VEGFA_HUMAN

兔

317

This assay has high sensitivity and excellent specificity for detection of Vascular Endothelial Growth Factor 121 (VEGF121). No significant cross-reactivity or interference between Vascular Endothelial Growth Factor 121 (VEGF121) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Sample Types: Serum, plasma and other biological fluids. Detection Range: 15.63-1000pg/mL. Sensitivity: The minimum detectable dose of this kit is typically less than 5.6pg/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vascular Endothelial Growth Factor 121 (VEGF121) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vascular Endothelial Growth Factor 121 (VEGF121) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vascular Endothelial Growth Factor 121 (VEGF121). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vascular Endothelial Growth Factor 121 (VEGF121). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vascular Endothelial Growth Factor 121 (VEGF121), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Vascular Endothelial Growth Factor 121 (VEGF121) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

6631029

AAF19659.1

34,406 Da

Angiogenesis Pathway 198772!!Axon Guidance Pathway 105688!!Bladder Cancer Pathway 83115!!Bladder Cancer Pathway 527!!Cellular Response To Hypoxia Pathway 645259!!Cellular Responses To Stress Pathway 645258!!Cytokine-cytokine Receptor Interaction Pathway 83051!!Cytokine-cytokine Receptor Interaction Pathway 460!!Developmental Biology Pathway 477129!!EPH-Ephrin Signaling Pathway 1127691

This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms. [provided by RefSeq, Jul 2008]

96 Strip Wells

800美元