ELISA/assay

胸腺肽乙4(Tb4)酶联免疫吸附试剂盒

MBS2024602

48-Strip-Wells

540美元

酶联免疫吸附试剂盒

胸腺肽乙4(Tb4)酶联免疫吸附试剂盒

胸腺肽乙4(Tb4)

TMSB4X; FX; PTMB4; TB4X; TMSB4; Thymosin,Beta 4,X-Linked; TB-500; Seraspenide; Hematopoietic system regulatory peptide

thymosin beta-4; Thymosin beta-4; thymosin beta-4; thymosin beta 4, X-linked; Fx

Tb4

TMSB4X;TMSB4X;FX;TB4X;PTMB4;TMSB4;TB4X;THYB4;TMSB4;T乙4

TYB4_HUMAN

人类

44

This assay has high sensitivity and excellent specificity for detection of Tb4. No significant cross-reactivity or interference between Tb4 and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Human serum, plasma and other biological fluids. Assay Type: Competitive. Detection Range: 2.47-200ng/mL. The standard curve concentrations used for the ELISA's were 200ng/mL, 66.67ng/mL, 22.22ng/mL, 7.41ng/mL, 2.47ng/mL. Sensitivity: 0.91ng/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tb4 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tb4 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of Tb4 in human serum, plasma and other biological fluids. Principle of the Assay This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Tb4 has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Tb4 and unlabeled Tb4 (Standards or samples) with the pre-coated antibody specific to Tb4. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Tb4 in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Tb4 in the sample.

11056061

NP_066932.1

NM_021109.3

P62328

5,053 Da

Hemostasis Pathway (106028); Platelet Activation, Signaling And Aggregation Pathway (106034); Platelet Degranulation Pathway (106050); Regulation Of Actin Cytoskeleton Pathway (198874); Regulation Of Actin Cytoskeleton Pathway (83089); Regulation Of Actin Cytoskeleton Pathway (500); Response To Elevated Platelet Cytosolic Ca2+ Pathway (106048)

This gene encodes an actin sequestering protein which plays a role in regulation of actin polymerization. The protein is also involved in cell proliferation, migration, and differentiation. This gene escapes X inactivation and has a homolog on chromosome Y. [provided by RefSeq, Jul 2008]

TMSB4X: Plays an important role in the organization of the cytoskeleton. Binds to and sequesters actin monomers (G actin) and therefore inhibits actin polymerization. Belongs to the thymosin beta family. Protein type: Motility/polarity/chemotaxis; Cytoskeletal. Chromosomal Location of Human Ortholog: Xq21.3-q22. Cellular Component: cytoskeleton; extracellular region. Molecular Function: actin monomer binding; protein binding. Biological Process: platelet activation; platelet degranulation; sequestering of actin monomers; actin filament organization; blood coagulation

48-Strip-Wells

540美元

96-Strip-Wells

730

5x96-Strip-Wells

2830

10x96-Strip-Wells

5080