抗体

单克隆抗体基质金属蛋白酶9(基质金属蛋白酶9)

MBS2025637

0.01毫克

90美元

单克隆

小鼠

未共轭

[16#]

免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀

抗体

单克隆抗体基质金属蛋白酶9(基质金属蛋白酶9)

[基质金属蛋白酶9]

[matrix metalloproteinase-9 preproprotein; Matrix metalloproteinase-9; matrix metalloproteinase-9; matrix metallopeptidase 9; 92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B; GELB]

[基质金属蛋白酶9]

[基质金属蛋白酶9;基质金属蛋白酶9;GELB;CLG4B;基质金属蛋白酶-9;MANDP2;CLG4B;基质金属蛋白酶-9;GELB]

单克隆

IgG2b Kappa

[16#]

小鼠

蛋白质A+蛋白质G亲和色谱

Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

1毫克/毫升

Storage: Avoid repeated freeze/thaw cycles. Store at 4ºC for frequent use. Aliquot and store at -20ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

免疫印迹;免疫组化;免疫细胞化学;免疫沉淀

Western blotting: 0.5-5 ug/mL;1:200-2000. Immunohistochemistry: 5-20 ug/mL;1:50-200. Immunocytochemistry: 5-20 ug/mL;1:50-200. Optimal working dilutions must be determined by end user.

免疫印迹(免疫印迹)

免疫印迹(免疫印迹)

基因敲除验证

Organism Species: Homo sapiens (Human). Source: Monoclonal antibody preparation. Traits: Liquid. Immunogen: Recombinant MMP9 (Gly213~Ala399) expressed in E.coli

Knock-Out Validation Antibody

74272287

NP_004985.2

NM_004994.2

P14780

AGE/RAGE Pathway (698754); Activation Of Matrix Metalloproteinases Pathway (576264); Angiogenesis Pathway (198772); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (730306); Bladder Cancer Pathway (83115); Bladder Cancer Pathway (527); CXCR4-mediated Signaling Events Pathway (137910); Collagen Formation Pathway (645288); Degradation Of Collagen Pathway (730309); Degradation Of Collagen Pathway (762258)

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. [provided by RefSeq, Jul 2008]

MMP9: May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly- -Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. Exists as monomer or homodimer; disulfide-linked. Exists also as heterodimer with a 25 kDa protein. Macrophages and transformed cell lines produce only the monomeric form. Interacts with ECM1. Activated by 4-aminophenylmercuric acetate and phorbol ester. Up-regulated by ARHGEF4, SPATA13 and APC via the JNK signaling pathway in colorectal tumor cells. Produced by normal alveolar macrophages and granulocytes. Inhibited by histatin-3 1/24 (histatin-5). Inhibited by ECM1. Belongs to the peptidase M10A family. Protein type: EC 3.4.24.35; Motility/polarity/chemotaxis; Protease; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 20q13.12. Cellular Component: extracellular exosome; extracellular region; extracellular space; ficolin-1-rich granule lumen; proteinaceous extracellular matrix; tertiary granule lumen. Molecular Function: collagen binding; endopeptidase activity; identical protein binding; metalloendopeptidase activity; metallopeptidase activity; protein binding; serine-type endopeptidase activity; zinc ion binding. Biological Process: cellular response to cadmium ion; cellular response to reactive oxygen species; collagen catabolic process; embryo implantation; endodermal cell differentiation; ephrin receptor signaling pathway; extracellular matrix disassembly; leukocyte migration; macrophage differentiation; negative regulation of apoptosis; negative regulation of cation channel activity; negative regulation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway; negative regulation of intrinsic apoptotic signaling pathway; neutrophil degranulation; ossification; positive regulation of cell migration; positive regulation of DNA binding; positive regulation of epidermal growth factor receptor signaling pathway; positive regulation of keratinocyte migration; positive regulation of protein phosphorylation; positive regulation of receptor binding; positive regulation of release of cytochrome c from mitochondria; positive regulation of vascular smooth muscle cell proliferation; proteolysis; skeletal system development. Disease: Metaphyseal Anadysplasia 2

0.01毫克

90美元

0.02毫克

100

0.05毫克

130

0.1毫克

160

0.2毫克

200

1毫克

430