产品简要
公司名称 :
MyBioSource
产品类型 :
蛋白
产品名称 :
重组肿瘤蛋白p53(TP53)
目录 :
MBS2030765
规格 :
0.01毫克
价格 :
150美元
更多信息或购买 :
图像
图像 1 :
MyBioSource MBS2030765 图像 1
图像 2 :
MyBioSource MBS2030765 图像 2
产品信息
目录号 :
MBS2030765
产品类型 :
重组蛋白
产品全称 :
重组肿瘤蛋白p53(TP53)
产品简称 :
[肿瘤蛋白p53]
其他名称 :
[Tumor protein p53; Cellular tumor antigen p53; cellular tumor antigen p53; tumor protein p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53]
产品基因名称 :
[TP53]
其他基因名称 :
[TP53;TP53;P53;BCC7;LFS1;TRP53;P53]
UniProt数据库进入名 :
P53_HUMAN
宿主 :
大肠杆菌
反应物种 :
大鼠
纯度 :
>98%
形式 :
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300
浓度 :
200微克/毫升
储存稳定性 :
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition.
检测过的应用 :
SDS-PAGE, WB, ELISA, IP, CoIP, ReporterAssays, Purification, Amine Reactive Labeling. (May be suitable for use in other assays to be determined by the end user.)
image1头 :
序列信息
image2头 :
SDS-Page
其它信息1 :
Source: Prokaryotic expression. Residues: Pro90~Ala354. Tags: N-terminal His-Tag. Tissue Specificity: Heart, Brain, Kidney, Liver. Subcellular Location: Cytoplasm. Nucleus. Endoplasmic reticulum. Mitochondrion matrix. Traits: Freeze-dried powder
其他信息2 :
Predicted isoelectric point: 5.9. Predicted Molecular Mass: 33.7kDa. Accurate Molecular Mass: 36kDa as determined by SDS-PAGE reducing conditions. Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
NCBI GI登录号 :
13097807
NCBI登录号 :
AAH03596.1
NCBI信号通路 :
AMPK Signaling Pathway (198868); Activation Of BH3-only Proteins Pathway (1270270); Activation Of NOXA And Translocation To Mitochondria Pathway (1270272); Activation Of PUMA And Translocation To Mitochondria Pathway (1270273); Alzheimers Disease Pathway (672448); Amyotrophic Lateral Sclerosis (ALS) Pathway (920975); Amyotrophic Lateral Sclerosis (ALS) Pathway (83099); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Apoptosis Pathway (198797); Apoptosis Pathway (83060)
NCBI总结 :
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons (PMIDs: 12032546, 20937277). [provided by RefSeq, Feb 2013]
UniProt数据库总结 :
p53: a transcription factor and major tumor suppressor that plays a major role in regulating cellular responses to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis. More than 50 percent of human tumors contain a mutation or deletion of the TP53 gene. p53 is modified post-translationally at multiple sites. DNA damage induces phosphorylation of p53 at S15, S20 and S37, reducing its interaction with the oncoprotein MDM2. MDM2 inhibits p53 accumulation by targeting it for ubiquitination and proteasomal degradation. Phosphorylated by many kinases including Chk2 and Chk1 at S20, enhancing its tetramerization, stability and activity. The phosphorylation by CAK at S392 is increased in human tumors and has been reported to influence the growth suppressor function, DNA binding and transcriptional activation of p53. Phosphorylation of p53 at S46 regulates the ability of p53 to induce apoptosis. The acetylation of p53 appears to play a positive role in the accumulation of p53 during the stress response. Following DNA damage, p53 becomes acetylated at K382, enhancing its binding to DNA. Deacetylation of p53 can occur through interaction with SIRT1, a deacetylase that may be involved in cellular aging and the DNA damage response. p53 regulates the transcription of a set of genes encoding endosomal proteins that regulate endosomal functions. These include STEAP3 and CHMP4C, which enhance exosome production, and CAV1 and CHMP4C, which produce a more rapid endosomal clearance of the EGFR from the plasma membrane. DNA damage regulates a p53-mediated secretory pathway, increasing the secretion of some proteins such as Hsp90, SERPINE1, SERPINB5, NKEF-A, and CyPA, and inhibiting the secretion of others including CTSL and IGFBP-2. Two alternatively spliced human isoforms have been reported. Isoform 2 is expressed in quiescent lymphocytes. Seems to be non-functional. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay. Protein type: DNA-binding; Motility/polarity/chemotaxis; Transcription factor; Nuclear receptor co-regulator; Activator; Tumor suppressor. Chromosomal Location of Human Ortholog: 17p13.1. Cellular Component: PML body; transcription factor TFIID complex; nuclear matrix; protein complex; mitochondrion; endoplasmic reticulum; replication fork; cytosol; nucleoplasm; nuclear body; mitochondrial matrix; cytoplasm; nuclear chromatin; nucleolus; nucleus; chromatin. Molecular Function: identical protein binding; protease binding; zinc ion binding; protein phosphatase 2A binding; p53 binding; protein N-terminus binding; receptor tyrosine kinase binding; protein phosphatase binding; transcription factor binding; protein kinase binding; histone acetyltransferase binding; protein binding; copper ion binding; enzyme binding; histone deacetylase regulator activity; DNA binding; protein heterodimerization activity; chaperone binding; ubiquitin protein ligase binding; damaged DNA binding; chromatin binding; transcription factor activity; ATP binding. Biological Process: viral reproduction; positive regulation of apoptosis; multicellular organismal development; positive regulation of transcription, DNA-dependent; T cell differentiation in the thymus; gastrulation; determination of adult life span; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; response to antibiotic; cellular response to glucose starvation; protein localization; negative regulation of neuroblast proliferation; base-excision repair; transforming growth factor beta receptor signaling pathway; cerebellum development; protein complex assembly; cell cycle arrest; ER overload response; response to X-ray; release of cytochrome c from mitochondria; somitogenesis; cell aging; chromatin assembly; circadian behavior; rRNA transcription; positive regulation of peptidyl-tyrosine phosphorylation; negative regulation of DNA replication; negative regulation of fibroblast proliferation; embryonic organ development; positive regulation of transcription from RNA polymerase II promoter; regulation of mitochondrial membrane permeability; negative regulation of transcription, DNA-dependent; regulation of tissue remodeling; negative regulation of apoptosis; G1 DNA damage checkpoint; transcription from RNA polymerase II promoter; DNA damage response, signal transduction by p53 class mediator; apoptosis; negative regulation of transcription from RNA polymerase II promoter; response to salt stress; entrainment of circadian clock by photoperiod; negative regulation of cell proliferation; positive regulation of protein oligomerization; positive regulation of histone deacetylation; DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; regulation of transcription, DNA-dependent; T cell proliferation during immune response; double-strand break repair; positive regulation of neuron apoptosis; response to gamma radiation; cell differentiation; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; protein tetramerization; mitochondrial DNA repair; Notch signaling pathway; in utero embryonic development; multicellular organism growth; B cell lineage commitment; cell proliferation; neuron apoptosis; T cell lineage commitment; negative regulation of helicase activity; protein import into nucleus, translocation; nucleotide-excision repair; Ras protein signal transduction; DNA strand renaturation; negative regulation of cell growth; negative regulation of transforming growth factor beta receptor signaling pathway; blood coagulation; response to DNA damage stimulus. Disease: Papilloma Of Choroid Plexus; Pancreatic Cancer; Nasopharyngeal Carcinoma; Li-fraumeni Syndrome 1; Breast Cancer; Osteogenic Sarcoma; Colorectal Cancer; Glioma Susceptibility 1; Adrenocortical Carcinoma, Hereditary; Hepatocellular Carcinoma; Basal Cell Carcinoma, Susceptibility To, 7
尺寸1 :
0.01毫克
价格1 :
150美元
尺寸2 :
0.05毫克
价格2 :
290
size3 :
0.1毫克
价格3 :
455
size4 :
0.2毫克
price4 :
560
size5 :
0.5毫克
price5 :
1080
size6 :
1毫克
price6 :
1610
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。