蛋白

亲环素A, 1-165aa, 人类, 重组, 大肠杆菌

MBS203127

0.1毫克

285美元

重组蛋白

亲环素A, 1-165aa, 人类, 重组, 大肠杆菌

亲环素A

Peptidylprolyl isomerase A; CYPA; CYPH; MGC12404; MGC23397; MGC117158; Cyclophilin A; Cyclosporin A binding protein; Peptidyl prolyl cis trans isomerase A; PPIA; PPIA protein; PPIase A; Rotamase A; RotamaseA; T cell cyclophilin.

peptidyl-prolyl cis-trans isomerase A isoform 1; Peptidyl-prolyl cis-trans isomerase A; peptidyl-prolyl cis-trans isomerase A; PPIase A; T cell cyclophilin; cyclosporin A-binding protein; epididymis secretory sperm binding protein Li 69p; rotamase A; peptidylprolyl isomerase A (cyclophilin A); Cyclophilin A; Cyclosporin A-binding protein; Rotamase APeptidyl-prolyl cis-trans isomerase A, N-terminally processed

PPIA;PPIA;CYPA;CYPH;HEL-S-69p;CYPA;PPIase A

PPIA_HUMAN

165

MGSSHHHHHH SSGLVPRGSH MVNPTVFFDI AVDGEPLGRV SFELFADKVP KTAENFRALS TGEKGFGYKG SCFHRIIPGF MCQGGDFTRH NGTGGKSIYG EKFEDENFIL KHTGPGILSM ANAGPNTNGS QFFICTAKTE WLDGKHVVFG KVKEGMNIVE AMERFGSRNG KTSKKITIAD CGQLE

> 95% by SDS - PAGE

在20mM三羟甲基氨基甲烷pH值8.0,.20mM氯化钠, 0.5mM DTT, 10% 甘油

1毫克/毫升(由布拉德福德Bradford检测确定)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

十二烷基磺酸钠-PAGE

Antigen Species: Human

Expression System: E Coli. Bioactivity: Specific activity is > 270 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25C in Tris-Hcl pH8.0 using chymotrypsin. Endotoxin: < 1.0 Eu per 1 microgram of protein (determined by LAL method). Assay: 1. Prepare 170ul assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin. 2. Add 10ul of recombinant Cyclophilin A protein with 1ug in 200 mM Tris-Hcl, pH 8.0. 3. Mix by inversion and equilibrate to 1C and monitor the A405nm until the value is constant using a spectrophotometer. 4. Add 20ul pre-chilled 5mM suc-AAFP-pNA dissolved in 3mM TFE, 460mM LiCl . 5. Record the increase in A405 nm for 30 minutes at 25C.

细胞周期

Cyclophilin A (also known as Peptidylprolyl isomerase A, PPIA) encodes a member of the peptidyl-prolyl cis-trans isomerase family. They are highly-conserved cytoplasmic enzymes that accelerate protein folding. Cyclophilin A is also incorporated into many viruses, including HIV-1, where it has been speculated to be involved in functions such as viral assembly and infectivity. Recombinant human PPIA, was expressed in E.coli and purified by using conventional chromatography techniques.

Holzman, T.F., et al, (1991). J. Biol. Chem. 266(4): 2474-2479. Berthoux L, et al, (2005). Proc Natl Acad Sci u S A. 102(41):14849-53.

10863927

NP_066953

NM_021130.4

20kDa (185aa), confirmed by MALDI-TOF.

APOBEC3G Mediated Resistance To HIV-1 Infection Pathway 106016!!Assembly Of The HIV Virion Pathway 106008!!Basigin Interactions Pathway 106065!!Binding And Entry Of HIV Virion Pathway 105986!!Budding And Maturation Of HIV Virion Pathway 106009!!Cell Surface Interactions At The Vascular Wall Pathway 106062!!Disease Pathway 530764!!Early Phase Of HIV Life Cycle Pathway 105985!!HIV Infection Pathway 105983!!HIV Life Cycle Pathway 105984

This gene encodes a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. The encoded protein is a cyclosporin binding-protein and may play a role in cyclosporin A-mediated immunosuppression. The protein can also interact with several HIV proteins, including p55 gag, Vpr, and capsid protein, and has been shown to be necessary for the formation of infectious HIV virions. Multiple pseudogenes that map to different chromosomes have been reported. [provided by RefSeq, Jul 2008]

0.1毫克

285美元