产品简要
公司名称 :
MyBioSource
产品类型 :
蛋白
产品名称 :
GFP, 1-238aa, Aequorea victoria, Recombinant, E Coli
目录 :
MBS203279
规格 :
0.1毫克
价格 :
290美元
更多信息或购买 :
产品信息
目录号 :
MBS203279
产品类型 :
重组蛋白
产品全称 :
GFP, 1-238aa, Aequorea victoria, Recombinant, E Coli
产品简称 :
绿色荧光蛋白
产品名称同义词 :
绿色荧光蛋白
其他名称 :
绿色荧光蛋白;绿色荧光蛋白
产品基因名称 :
绿色荧光蛋白
其他基因名称 :
绿色荧光蛋白
UniProt数据库进入名 :
GFP_AEQVI
序列长度 :
238
序列 :
MSKGEELFTG VVPILVELDG DVNGHKFSVS GEGEGDATYG KLTLKFICTT GKLPVPWPTL VTTFSYGVQC FSRYPDHMKQ HDFFKSAMPE GYVQERTIFF KDDGNYKTRA EVKFEGDTLV NRIELKGIDF KEDGNILGHK LEYNYNSHNV YIMADKQKNG IKVNFKIRHN IEDGSVQLAD HYQQNTPIGD GPVLLPDNHY LSTQSALSKD PNEKRDHMVL LEFVTAAGIT HGMDELYK
纯度 :
> 95% by SDS - PAGE
形式 :
液体。 在20 mM三羟甲基氨基甲烷氯化氢缓冲液(pH值8.0)含10% 甘油
浓度 :
1毫克/毫升(由布拉德福德Bradford检测确定)
储存稳定性 :
Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
检测过的应用 :
十二烷基磺酸钠-PAGE
其它信息1 :
Antigen Species: Aequorea victoria
其他信息2 :
Expression System: E Coli. Endotoxin: < 1.0 Eu per 1 microgram of protein (determined by LAL method). Analysis: 1. Prepare a 150ul recombinant GFP with various concentrations (0.00074 nM - 6.21uM) in assay buffer and equilibrate to 25C. (Assay buffer: 10 mM Tris-HCl (pH 8.0), 10 mM EDTA, 0.02% sodium azide.) . 2. Read at excitation wavelengths 485nm and emission 535nm. - 96 Well Polystyrene Microplate, black (greiner bio-one) . - Fluorescent plate reader (PerkinEmerl, VICTOR X3)
产品种类 :
Fusion Tag & Detection
产品描述 :
GFP, also known as green fluorescent protein, is a protein produced by the jellyfish (Aequorea Victoria) that emits bioluminescence in the green zone of the visible spectrum. GFP has become a useful and ubiquitous tool for making chimeric proteins, where it functions as a fluorescent protein tag. It has been expressed in most known cell types and is used as a noninvasive fluorescent marker in living cells and organisms. This protein enables a wide range of applications where it has functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Recombinant GFP protein was expressed in E.coli and purified by using conventional chromatography.
产品引用 :
Millan MI, et al. (2009). Medicina. 69(3):370-4. Marshall J, et al. (1995). Neuron, 14(2):211-5.
NCBI GI登录号 :
1169893
NCBI登录号 :
P42212.1
UniProt数据库登录号 :
P42212
NCBI分子量 :
26.8 kDa (238aa), confirmed by MALDI-TOF.
尺寸1 :
0.1毫克
价格1 :
290美元
尺寸2 :
0.5毫克
价格2 :
650
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华，公司愿景是“生物研究试剂的源头”，现在位于圣地亚哥市。