产品简要
公司名称 :
MyBioSource
产品类型 :
蛋白
产品名称 :
ALDH2, 18-517aa, 人类, 重组, 大肠杆菌
目录 :
MBS203287
规格 :
0.1毫克
价格 :
255美元
更多信息或购买 :
产品信息
目录号 :
MBS203287
产品类型 :
重组蛋白
产品全称 :
ALDH2, 18-517aa, 人类, 重组, 大肠杆菌
产品简称 :
ALDH2
产品名称同义词 :
ALDH-E2; ALDHI; ALDM; Aldehyde dehydrogenase; mitochondrial Acetaldehyde dehydrogenase 2; Aldehyde dehydrogenase 2 family; Aldehyde dehydrogenase 2 family (mitochondrial); Aldehyde dehydrogenase mitochondrial; ALDH 2; ALDH class 2; ALDH E2; Liver mitochondrial ALDH; MGC1806; Mitochondrial aldehyde dehydrogenase 2; Nucleus encoded mitochondrial aldehyde dehydrogenase 2.
其他名称 :
aldehyde dehydrogenase, mitochondrial isoform 1; Aldehyde dehydrogenase, mitochondrial; aldehyde dehydrogenase, mitochondrial; ALDH class 2; acetaldehyde dehydrogenase 2; liver mitochondrial ALDH; nucleus-encoded mitochondrial aldehyde dehydrogenase 2; aldehyde dehydrogenase 2 family (mitochondrial); ALDH class 2; ALDH-E2; ALDHI
产品基因名称 :
ALDH2
其他基因名称 :
ALDH2;ALDH2;ALDM;ALDHI;ALDH-E2;ALDM
UniProt数据库进入名 :
ALDH2_HUMAN
序列长度 :
517
序列 :
MSAAATQAVP APNQQPEVFC NQIFINNEWH DAVSRKTFPT VNPSTGEVIC QVAEGDKEDV DKAVKAARAA FQLGSPWRRM DASHRGRLLN RLADLIERDR TYLAALETLD NGKPYVISYL VDLDMVLKCL RYYAGWADKY HGKTIPIDGD FFSYTRHEPV GVCGQIIPWN FPLLMQAWKL GPALATGNVV VMKVAEQTPL TALYVANLIK EAGFPPGVVN IVPGFGPTAG AAIASHEDVD KVAFTGSTEI GRVIQVAAGS SNLKRVTLEL GGKSPNIIMS DADMDWAVEQ AHFALFFNQG QCCCAGSRTF VQEDIYDEFV ERSVARAKSR VVGNPFDSKT EQGPQVDETQ FKKILGYINT GKQEGAKLLC GGGIAADRGY FIQPTVFGDV QDGMTIAKEE IFGPVMQILK FKTIEEVVGR ANNSTYGLAA AVFTKDLDKA NYLSQALQAG TVWVNCYDVF GAQSPFGGYK MSGSGRELGE YGLQAYTEVK TVTVKVPQKN S
纯度 :
> 90% by SDS-PAGE
形式 :
液体。 在20 mM三羟甲基氨基甲烷氯化氢缓冲液(pH值7.5)含1mM DTT, 1 mM乙二胺四乙酸, 10% 甘油
浓度 :
1毫克/毫升(由布拉德福德Bradford检测确定)
储存稳定性 :
Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
检测过的应用 :
十二烷基磺酸钠-PAGE
其它信息1 :
Antigen Species: Human
其他信息2 :
Expression System: E Coli. Bioactivity: Specific activity is > 0.14 units/ml, and was obtained by measuring the increase of NADP in absorbance at 340 nm resulting from the reduction of NAD. One unit will oxidize 1.0 umole of acetaldehyde to acetic acid per minute at pH 8.0 at 25C in the presence of beta-NAD, potassium and thiols. Endotoxin: < 1.0 Eu per 1 microgram of protein (determined by LAL method). Assay: 1. Prepare a 3ml reaction mixture into a suitable container: The final concentrations are 103 mM Tris, 0.67 mM beta-NAD, 100 mM potassium chloride, 10 mM 2-mercaptoethanol, 2 mM acetaldehyde, 0.0007 % (w/v) BSA. 2. Equilibrate to 25C and monitor at A340nm until value is constant using a spectrophotometer. 3. Add 5ug of recombinant ALDH2 into reaction mixture and mix immediately. 4. Record the increase at A340nm for 5 minutes.
产品种类 :
Redox Proteins
产品描述 :
ALDH2 (Aldehyde dehydrogenase 2 family) belongs to the aldehyde dehydrogenase family which catalyze the chemical transformation from acetaldehyde to acetic acid and is the second enzyme of the major oxidative pathway of alcohol metabolism. There are two major liver isoforms of this enzyme, cytosolic and mitochondrial, and they can be also distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Recombinant human ALDH2 protein was expressed in E.coli and purified by using conventional chromatography techniques.
产品引用 :
Crabb DW., et al. (1989). J Clin Invest. 83(1):314-6. Feng Liu., et al. (2002). Plant Physiol. 130(4):1657-1674.
NCBI GI登录号 :
25777732
NCBI登录号 :
NP_000681
NCBI基因登录号 :
NM_000690.3
NCBI分子量 :
54.5 kDa (501aa), confirmed by MALDI-TOF.
NCBI信号通路 :
Arginine And Proline Metabolism Pathway (82957); Arginine And Proline Metabolism Pathway (323); Ascorbate And Aldarate Metabolism Pathway (82932); Ascorbate And Aldarate Metabolism Pathway (293); Biological Oxidations Pathway (105698); Defective CYP11A1 Causes Adrenal Insufficiency, Congenital, With 46,XY Sex Reversal (AICSR) Pathway (1127640); Defective CYP11B1 Causes Adrenal Hyperplasia 4 (AH4) Pathway (1127641); Defective CYP11B2 Causes Corticosterone Methyloxidase 1 Deficiency (CMO-1 Deficiency) Pathway (1127642); Defective CYP17A1 Causes Adrenal Hyperplasia 5 (AH5) Pathway (1127643); Defective CYP19A1 Causes Aromatase Excess Syndrome (AEXS) Pathway (1127644)
NCBI总结 :
This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of aldehyde dehydrogenase, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have the cytosolic isozyme but not the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of a catalytically active form of the mitochondrial isozyme. The increased exposure to acetaldehyde in individuals with the catalytically inactive form may also confer greater susceptibility to many types of cancer. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Mar 2011]
UniProt数据库总结 :
ALDH2: This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of aldehyde dehydrogenase, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have the cytosolic isozyme but not the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of a catalytically active form of the mitochondrial isozyme. The increased exposure to acetaldehyde in individuals with the catalytically inactive form may also confer greater susceptibility to many types of cancer. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Mar 2011]. Protein type: Amino Acid Metabolism - tryptophan; Carbohydrate Metabolism - propanoate; Amino Acid Metabolism - histidine; Carbohydrate Metabolism - ascorbate and aldarate; Other Amino Acids Metabolism - beta-alanine; Carbohydrate Metabolism - pyruvate; Oxidoreductase; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Mitochondrial; Lipid Metabolism - glycerolipid; Amino Acid Metabolism - lysine degradation; Lipid Metabolism - fatty acid; Secondary Metabolites Metabolism - limonene and pinene degradation; EC 1.2.1.3; Amino Acid Metabolism - arginine and proline; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - butanoate. Chromosomal Location of Human Ortholog: 12q24.2. Cellular Component: mitochondrial matrix. Molecular Function: aldehyde dehydrogenase (NAD) activity; electron carrier activity; aldehyde dehydrogenase [NAD(P)+] activity. Biological Process: synaptic transmission; ethanol catabolic process; xenobiotic metabolic process; alcohol metabolic process; carbohydrate metabolic process; ethanol oxidation; neurotransmitter biosynthetic process. Disease: Alcohol Sensitivity, Acute
尺寸1 :
0.1毫克
价格1 :
255美元
尺寸2 :
0.5毫克
价格2 :
535
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。