产品简要
公司名称 :
MyBioSource
产品类型 :
蛋白
产品名称 :
细胞周期蛋白H, 1- 323aa, 人类, 多聚组氨酸标签, 重组, 大肠杆菌
目录 :
MBS203429
规格 :
0.1毫克
价格 :
285美元
更多信息或购买 :
产品信息
目录号 :
MBS203429
产品类型 :
重组蛋白
产品全称 :
细胞周期蛋白H, 1- 323aa, 人类, 多聚组氨酸标签, 重组, 大肠杆菌
产品简称 :
细胞周期蛋白H
产品名称同义词 :
CCNH; CAK; p34; p37; 6330408H09Rik; AI661354; AV102684; AW538719; CDK activating kinase; Cyclin dependent kinase activating kinase; Cyclin H; CyclinH; MO15 associated protein; P36; Cyclin-H
其他名称 :
cyclin-H isoform 1; Cyclin-H; cyclin-H; CAK complex subunit; CDK-activating kinase complex subunit; MO15-associated protein; cyclin-dependent kinase-activating kinase complex subunit; cyclin H; MO15-associated protein; p34; p37
其他基因名称 :
CCNH; CCNH; CAK; p34; p37; CycH
UniProt数据库进入名 :
CCNH_HUMAN
序列长度 :
323
序列 :
MGSSHHHHHH SSGLVPRGSH MYHNSSQKRH WTFSSEEQLA RLRADANRKF RCKAVANGKV LPNDPVFLEP HEEMTLCKYY EKRLLEFCSV FKPAMPRSVV GTACMYFKRF YLNNSVMEYH PRIIMLTCAF LACKVDEFNV SSPQFVGNLR ESPLGQEKAL EQILEYELLL IQQLNFHLIV HNPYRPFEGF LIDLKTRYPI LENPEILRKT ADDFLNRIAL TDAYLLYTPS QIALTAILSS ASRAGITMES YLSESLMLKE NRTCLSQLLD IMKSMRNLVK KYEPPRSEEV AVLKQKLERC HSAELALNVI TKKRKGYEDD DYVSKKSKHE EEEWTDDDLV ESL
纯度 :
> 90% by SDS - PAGE
形式 :
液体。 在20mM三羟甲基氨基甲烷氯化氢缓冲液(pH值8.0)含2mM DTT, 30% 甘油, 2mM乙二胺四乙酸, 0.1M氯化钠
浓度 :
1毫克/毫升(由布拉德福德Bradford检测确定)
储存稳定性 :
Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
检测过的应用 :
十二烷基磺酸钠-PAGE
其它信息1 :
Antigen Species: Human. Tag: His-tag
其他信息2 :
Expression System: E Coli
产品种类 :
细胞周期
产品描述 :
Cyclin H, also known as CDK7, is a member of the cyclin-dependent protein kinase family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin H forms a trimeric complex with cyclin H and MAT1, which functions as a CDK-activating kinase (CAK). Cyclin H is an essential component of the transcription factor TFIIH that is involved in transcription initiation and DNA repair. Recombinant human Cyclin H, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
产品引用 :
Schneider, E., et al. (1998) Oncogene. 17(21):2733-41. Yee A., et al. (1995) Canser Res. 55(24):6058-62.
NCBI GI登录号 :
4502623
NCBI登录号 :
NP_001230
NCBI基因登录号 :
NM_001239.3
NCBI分子量 :
39.8 kDa (343aa), confirmed by MALDI-TOF
NCBI信号通路 :
Basal Transcription Factors Pathway (83038); Basal Transcription Factors Pathway (443); Cell Cycle Pathway (530733); Cell Cycle, Mitotic Pathway (105765); Cell Cycle Pathway (198811); Cell Cycle Pathway (83054); Cell Cycle Pathway (463); Cyclin A/B1 Associated Events During G2/M Transition Pathway (105802); Cyclin A:Cdk2-associated Events At S Phase Entry Pathway (105783); Cyclin D Associated Events In G1 Pathway (105767)
NCBI总结 :
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK7 kinase and ring finger protein MAT1. The kinase complex is able to phosphorylate CDK2 and CDC2 kinases, thus functions as a CDK-activating kinase (CAK). This cyclin and its kinase partner are components of TFIIH, as well as RNA polymerase II protein complexes. They participate in two different transcriptional regulation processes, suggesting an important link between basal transcription control and the cell cycle machinery. A pseudogene of this gene is found on chromosome 4. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Nov 2010]
UniProt数据库总结 :
CCNH: Regulates CDK7, the catalytic subunit of the CDK- activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle. Associates primarily with CDK7 and MAT1 to form the CAK complex. CAK can further associate with the core-TFIIH to form the TFIIH basal transcription factor. Belongs to the cyclin family. Cyclin C subfamily. Protein type: Cell cycle regulation. Chromosomal Location of Human Ortholog: 5q13.3-q14. Cellular Component: nucleoplasm; holo TFIIH complex; nucleus; cyclin-dependent protein kinase activating kinase holoenzyme complex. Molecular Function: RNA polymerase subunit kinase activity; DNA-dependent ATPase activity; protein binding; cyclin-dependent protein kinase regulator activity; protein kinase binding. Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; viral reproduction; positive regulation of viral transcription; transcription from RNA polymerase I promoter; RNA elongation from RNA polymerase I promoter; termination of RNA polymerase I transcription; DNA repair; protein amino acid phosphorylation; regulation of gene expression, epigenetic; mRNA capping; negative regulation of gene expression, epigenetic; nucleotide-excision repair; transcription-coupled nucleotide-excision repair; RNA elongation from RNA polymerase II promoter; gene expression; positive regulation of transcription from RNA polymerase II promoter; nucleotide-excision repair, DNA damage removal; regulation of cyclin-dependent protein kinase activity; mitotic cell cycle; G2/M transition of mitotic cell cycle; transcription initiation from RNA polymerase I promoter; G1/S transition of mitotic cell cycle
尺寸1 :
0.1毫克
价格1 :
285美元
尺寸2 :
0.5毫克
价格2 :
640
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。