蛋白

OGG1, 1-345aa, 人类, 多聚组氨酸标签, 大肠杆菌

MBS203473

0.1毫克

310美元

重组蛋白

OGG1, 1-345aa, 人类, 多聚组氨酸标签, 大肠杆菌

OGG1

HMMH; HOGG1; MuTM; OGH1; AP lyase; 8-oxoguanine DNA glycosylase 1

8-oxoguanine DNA glycosylase; N-glycosylase/DNA lyase; N-glycosylase/DNA lyase; 8-hydroxyguanine DNA glycosylase; AP lyase; DNA-apurinic or apyrimidinic site lyase; OGG1 type 1f; 8-oxoguanine DNA glycosylase

OGG1

OGG1;OGG1;HMMH;MUTM;OGH1;HOGG1;MMH;MUTM;OGH1;ap裂解酶

OGG1_HUMAN

345

MGSSHHHHHH SSGLVPRGSH TGSMPARALL PRRMGHRTLA STPALWASIP CPRSELRLDL VLPSGQSFRW REQSPAHWSG VLADQVWTLT QTEEQLHCTV YRGDKSQASR PTPDELEAVR KYFQLDVTLA QLYHHWGSVD SHFQEVAQKF QGVRLLRQDP IECLFSFICS SNNNIARITG MVERLCQAFG PRLIQLDDVT YHGFPSLQAL AGPEVEAHLR KLGLGYRARY VSASARAILE EQGGLAWLQQ LRESSYEEAH KALCILPGVG TKVADCICLM ALDKPQAVPV DVHMWHIAQRDYSWHPTTSQ AKGPSPQTNK ELGNFFRSLW GPYAGWAQAV LFSADLRQCR HAQEPPAKRR KGSKGPEG

> 90% by SDS - PAGE

液体。 在20 mM三羟甲基氨基甲烷氯化氢缓冲液(pH值8.0)含100 mM氯化钠, 40% 甘油

0.5毫克/毫升(由布拉德福德Bradford检测确定)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

十二烷基磺酸钠-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli

酶&蛋白酶

OGG1, also known as 8-oxoguanine glycosylase, is a DNA glycosylase enzyme involved in base excision repair. This protein is the primary enzyme responsible for the excision of 7,8-dihydro-8-oxoguanine (8-oxoG), a mutagenic base byproduct which occurs as a result of exposure to reactive oxygen species (ROS). It has a beta lyase activity that nicks DNA 3' to the lesion. Recombinant human OGG1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Seeberg E., et al. (2002). Nucleic Acids Res. 30(11):2349-57. Hodges NJ., et al (2010). DNA Repair (Amst). 9(2):144-52.

12653743

AAH00657

41.2 kDa(368aa), confirmed by MALDI-TOF

Base Excision Repair Pathway (105838); Base Excision Repair Pathway (83043); Base Excision Repair Pathway (451); Base-Excision Repair, AP Site Formation Pathway (105839); Base-free Sugar-phosphate Removal Via The Single-nucleotide Replacement Pathway (105848); Cleavage Of The Damaged Purine Pathway (105842); DNA Repair Pathway (105837); Depurination Pathway (105840); Displacement Of DNA Glycosylase By APE1 Pathway (105849); Recognition And Association Of DNA Glycosylase With Site Containing An Affected Purine Pathway (105841)

This gene encodes the enzyme responsible for the excision of 8-oxoguanine, a mutagenic base byproduct which occurs as a result of exposure to reactive oxygen. The action of this enzyme includes lyase activity for chain cleavage. Alternative splicing of the C-terminal region of this gene classifies splice variants into two major groups, type 1 and type 2, depending on the last exon of the sequence. Type 1 alternative splice variants end with exon 7 and type 2 end with exon 8. All variants share the N-terminal region in common, which contains a mitochondrial targeting signal that is essential for mitochondrial localization. Many alternative splice variants for this gene have been described, but the full-length nature for every variant has not been determined. [provided by RefSeq, Aug 2008]

OGG1: DNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N- methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta- lyase activity that nicks DNA 3 to the lesion. Defects in OGG1 may be a cause of renal cell carcinoma (RCC). It is a heterogeneous group of sporadic or hereditary carcinoma derived from cells of the proximal renal tubular epithelium. It is subclassified into clear cell renal carcinoma (non-papillary carcinoma), papillary renal cell carcinoma, chromophobe renal cell carcinoma, collecting duct carcinoma with medullary carcinoma of the kidney, and unclassified renal cell carcinoma. Belongs to the type-1 OGG1 family. 8 isoforms of the human protein are produced by alternative splicing. Protein type: EC 4.2.99.18; Lyase; DNA repair, damage; Deoxyribonuclease. Chromosomal Location of Human Ortholog: 3p26.2. Cellular Component: nucleoplasm; nuclear matrix; mitochondrion; nuclear speck. Molecular Function: protein binding; endonuclease activity; microtubule binding; DNA N-glycosylase activity; oxidized purine base lesion DNA N-glycosylase activity; damaged DNA binding; 8-oxo-7,8-dihydroguanine DNA N-glycosylase activity. Biological Process: response to drug; depurination; DNA repair; DNA catabolic process, endonucleolytic; response to estradiol stimulus; response to radiation; response to ethanol; regulation of transcription, DNA-dependent; base-excision repair, AP site formation; base-excision repair; nucleotide-excision repair; response to folic acid; regulation of protein import into nucleus, translocation; response to oxidative stress; acute inflammatory response; aging; negative regulation of apoptosis. Disease: Renal Cell Carcinoma, Nonpapillary

0.1毫克

310美元

0.5毫克

720