蛋白

缺氧诱导因子1A, 1-85aa, 人类, 多聚组氨酸标签, 大肠杆菌

MBS203569

0.01毫克

310美元

重组蛋白

缺氧诱导因子1A, 1-85aa, 人类, 多聚组氨酸标签, 大肠杆菌

缺氧诱导因子1A

BHLHE78;MOP1;PASD8;缺氧诱导因子甲

hypoxia-inducible factor 1-alpha isoform 1; Hypoxia-inducible factor 1-alpha; hypoxia-inducible factor 1-alpha; ARNT interacting protein; ARNT-interacting protein; HIF-1-alpha; PAS domain-containing protein 8; basic-helix-loop-helix-PAS protein MOP1; class E basic helix-loop-helix protein 78; hypoxia-inducible factor 1 alpha isoform I.3; hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia-inducible factor1alpha; member of PAS protein 1; member of PAS superfamily 1; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78; bHLHe78; Member of PAS protein 1; PAS domain-containing protein 8

缺氧诱导因子1A

缺氧诱导因子1A;缺氧诱导因子1A;HIF1;MOP1;PASD8;HIF-1A;bHLHe78;HIF-1alpha;HIF1-甲;BHLHE78;MOP1;PASD8;HIF-1;HIF1甲;bHLHe78

HIF1A_HUMAN

826

MGSSHHHHHH SSGLVPRGSH MEGAGGANDK KKISSERRKE KSRDAARSRR SKESEVFYEL AHQLPLPHNV SSHLDKASVM RLTISYLRVR KLLDAGDLDI EDDMK

> 80 % by SDS - PAGE

Liquid. In 20mM Tris buffer(pH 8.0) containing 20% glycerol, 1mM DTT, 0.2M NaCl, 1mM EDTA.

0.25毫克/毫升(由布拉德福德Bradford检测确定)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

十二烷基磺酸钠-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli

转录&翻译

Hypoxia-inducible factor-1 (HIF-1), identified as one of the transcription factors, has been found to play an essential role in oxygen homeostasis. HIF-1 is a heterodimer composed of HIF-1 beta subunit and one of three subunits (Hif-1 alpha, Hif-2 alpha or Hif-3 alpha). The activation of Hif-1 alpha is closely associated with a variety of tumors and oncogenic pathways. Hif-1 alpha consists of DNA binding domain(DBD domain), dimerization domain and C-terminal regulatory domains, including two transactivation domains(TAD), an oxygen-dependent degradation(ODD) domain, and inhibitory domains. Recombinant human Hif-1 alpha (1-85) protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Okuyama H., et al. (2006) J Biol Chem. 281(22):15554-63.

4504385

NP_001521

NM_001530.3

11.8 kDa (105aa), confirmed by MALDI-TOF (molecular weight on SDS-PAGE will appear higher)

AGE/RAGE Pathway 698754!!Adipogenesis Pathway 198832!!Angiogenesis Pathway 198772!!Cellular Response To Hypoxia Pathway 645259!!Cellular Responses To Stress Pathway 645258!!Central Carbon Metabolism In Cancer Pathway 1059538!!Central Carbon Metabolism In Cancer Pathway 1084231!!Choline Metabolism In Cancer Pathway 1059539!!Choline Metabolism In Cancer Pathway 1084232!!Circadian Clock Pathway 187173

This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2011]

0.01毫克

310美元