蛋白

ARG1, 1-322aa, 人类, 多聚组氨酸标签, 大肠杆菌

MBS203664

0.1毫克

285美元

重组蛋白

ARG1, 1-322aa, 人类, 多聚组氨酸标签, 大肠杆菌

ARG1

Arginase-1; liver; Arginase-1; liver A I; Al; ARG 1; ARG1; Arginase 1; Arginase liver; Arginase type I; Arginase1; Liver type arginase; Type I arginase.

arginase-1 isoform 2; Arginase-1; arginase-1; arginase, liver; liver-type arginase; type I arginase; arginase 1; Liver-type arginase; Type I arginase

ARG1

ARG1;ARG1

ARGI1_HUMAN

322

MSAKSRTIGI IGAPFSKGQP RGGVEEGPTV LRKAGLLEKL KEQECDVKDY GDLPFADIPN DSPFQIVKNP RSVGKASEQL AGKVAEVKKN GRISLVLGGD HSLAIGSISG HARVHPDLGV IWVDAHTDIN TPLTTTSGNL HGQPVSFLLK ELKGKIPDVP GFSWVTPCIS AKDIVYIGLR DVDPGEHYIL KTLGIKYFSM TEVDRLGIGK VMEETLSYLL GRKKRPIHLS FDVDGLDPSF TPATGTPVVG GLTYREGLYI TEEIYKTGLL SGLDIMEVNP SLGKTPEEVT RTVNTAVAIT LACFGLAREG NHKPIDYLNP PKLEHHHHHH

> 85% by SDS - PAGE

液体。 20mM三羟甲基氨基甲烷氯化氢缓冲液(pH8.0)含20% 甘油, 2mM DTT, 100mM氯化钠

0.5毫克/毫升(由布拉德福德Bradford检测确定)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

十二烷基磺酸钠-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli

代谢

Arginase is a manganese-containing enzyme which catalyzes the hydrolysis of arginine to ornithine and urea. It is the final enzyme of the urea cycle. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform functions in the urea cycle, and is located primarily in the cytoplasm of the liver. The type II isoform has been implicated in the regulation of the arginine/ornithine concentrations in the cell. It is located in mitochondria of several tissues in the body, with most abundance in the kidney and prostate. Recombinant human ARG1, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Wu G., et al (1998) The Biochemical journal 336 (Pt 1): 1-17. Iyer RK., et al (2002) Molecular and cellular biology 22 (13): 4491-8.

10947139

NP_000036

NM_000045.3

35.8kDa (330aa), confirmed by MALDI-TOF

ATF-2 Transcription Factor Network Pathway (138006); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Arginine And Proline Metabolism Pathway (82957); Arginine And Proline Metabolism Pathway (323); Biosynthesis Of Amino Acids Pathway (790012); Biosynthesis Of Amino Acids Pathway (795174); IL4-mediated Signaling Events Pathway (137933); Metabolic Pathways (132956); Metabolism Pathway (477135)

Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

ARG1: Homotrimer. By arginine or homoarginine. Belongs to the arginase family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Hydrolase; Amino Acid Metabolism - arginine and proline; EC 3.5.3.1. Chromosomal Location of Human Ortholog: 6q23. Cellular Component: extracellular space; neuron projection; cell soma; cytoplasm; cytosol; nucleus. Molecular Function: manganese ion binding; arginase activity. Biological Process: response to drug; mammary gland involution; maternal process involved in pregnancy; response to herbicide; liver development; response to amino acid stimulus; response to vitamin A; response to manganese ion; response to vitamin E; response to selenium ion; response to cadmium ion; response to zinc ion; response to methylmercury; arginine catabolic process; positive regulation of endothelial cell proliferation; response to axon injury; collagen biosynthetic process; lung development; urea cycle; response to amine stimulus. Disease: Argininemia

0.1毫克

285美元

0.5毫克

640