蛋白

RPA2, 1-270aa, 人类, 多聚组氨酸标签, 大肠杆菌

MBS203846

0.05毫克

375美元

重组蛋白

RPA2, 1-270aa, 人类, 多聚组氨酸标签, 大肠杆菌

RPA2

Replication protein A 32 kDa subunit; REPA2; RPA32; RPA32; RP-A p32; RP-A p34

replication protein A 32 kDa subunit isoform 1; Replication protein A 32 kDa subunit; replication protein A 32 kDa subunit; RF-A protein 2; replication factor A protein 2; replication protein A 34 kDa subunit; replication protein A2, 32kDa; Replication factor A protein 2; RF-A protein 2; Replication protein A 34 kDa subunit; RP-A p34

RPA2

RPA2;RPA2;REPA2;RPA32;RP-A p32;RP-A p34;REPA2;RPA32;RPA34;RP-A p32;RF-A蛋白2;RP-A p34

RFA2_HUMAN

270

MGSSHHHHHH SSGLVPRGSH MGSMWNSGFE SYGSSSYGGA GGYTQSPGGF GSPAPSQAEK KSRARAQHIV PCTISQLLSA TLVDEVFRIG NVEISQVTIV GIIRHAEKAP TNIVYKIDDM TAAPMDVRQW VDTDDTSSEN TVVPPETYVK VAGHLRSFQN KKSLVAFKIM PLEDMNEFTT HILEVINAHM VLSKANSQPS AGRAPISNPG MSEAGNFGGN SFMPANGLTV AQNQVLNLIK ACPRPEGLNF QDLKNQLKHM SVSSIKQAVD FLSNEGHIYS TVDDDHFKST DAE

> 85 % by SDS - PAGE

液体。 在20mM三羟甲基氨基甲烷氯化氢缓冲液(pH值8.0)含10% 甘油, 2mM DTT, 0.1M氯化钠

0.5毫克/毫升(由布拉德福德Bradford检测确定)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

十二烷基磺酸钠-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli

Epigenomics

Replication Protein A (RPA) is a single stranded DNA binding protein. Human RPA is a heterotrimeric protein containing subunits of 14, 32 and 70kDa. This protein complex is highly conserved in eukaryotes and is essential in DNA replication, homologous recombination and nucleotide excision repair. The C-terminus of RPA2 can specfically intereact with the DNA repair enzyme uNG2 and repair factors XPA and Rad52, each of which functions in a different repair pathway. In addition, this protein binds specifically to the SH2 domain of Stat3 in vivo, and overexpression of RPA2 corresponds to the augmented growth factor-stimulated tyrosine phosphorylation and transcription activities of Stat3. Recombinant human RPA2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Wang M., et al. (2000) Biochemistry. 39(21):6433-9. Mer G., et al. (2000) Cell. 103(3):449-56.

4506585

NP_002937

NM_002946.4

31.7 kDa (293aa) confirmed by MALDI-TOF

Activation Of ATR In Response To Replication Stress Pathway (105754); Activation Of The Pre-replicative Complex Pathway (105777); Assembly Of The RAD51-ssDNA Nucleoprotein Complex Pathway (105873); Cell Cycle Pathway (530733); Cell Cycle Checkpoints Pathway (105739); Cell Cycle, Mitotic Pathway (105765); Cellular Response To Heat Stress Pathway (1127553); Cellular Responses To Stress Pathway (645258); Chromosome Maintenance Pathway (161048); DNA Repair Pathway (105837)

RFA2: Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Required for the efficient recruitment of the DNA double-strand break repair factor RAD51 to chromatin in response to DNA damage. Heterotrimer of 70, 32 and 14 kDa chains (canonical replication protein A complex). Component of the alternative replication protein A complex (aRPA) composed of RPA1, RPA3 and RPA4. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Binds to SERTAD3/RBT1. Interacts with TIPIN. Directly interacts with PPP4R2, but not with SMEK2; this interaction is DNA damage-dependent and leads RPA2 dephosphorylation by PPP4C recruitment. Interacts with RAD51, preferentially when hyperphosphorylated. Directly interacts with RFWD3. 3 isoforms of the human protein are produced by alternative splicing. Protein type: DNA replication. Chromosomal Location of Human Ortholog: 1p35. Cellular Component: nucleoplasm; chromosome, telomeric region; PML body; DNA replication factor A complex; nucleus. Molecular Function: protein binding; enzyme binding; ubiquitin protein ligase binding; damaged DNA binding; protein N-terminus binding; protein phosphatase binding; single-stranded DNA binding. Biological Process: G1 DNA damage checkpoint; mismatch repair; DNA strand elongation during DNA replication; DNA repair; double-strand break repair via homologous recombination; telomere maintenance via semi-conservative replication; DNA recombinase assembly; nucleotide-excision repair; base-excision repair; double-strand break repair; transcription-coupled nucleotide-excision repair; telomere maintenance via recombination; nucleotide-excision repair, DNA damage removal; nucleotide-excision repair, DNA gap filling; mitotic cell cycle; DNA replication; telomere maintenance; G1/S transition of mitotic cell cycle

0.05毫克

375美元

0.25毫克

925