蛋白

ALDOB, 1-364aa, 人类, 多聚组氨酸标签, 大肠杆菌

MBS204182

0.1毫克

310美元

重组蛋白

ALDOB, 1-364aa, 人类, 多聚组氨酸标签, 大肠杆菌

ALDOB

果糖二磷酸醛缩酶B;ALDB;ALDO2;果糖二磷酸醛缩酶B

fructose-bisphosphate aldolase B; Fructose-bisphosphate aldolase B; fructose-bisphosphate aldolase B; aldolase 2; aldolase B, fructose-bisphosphatase; liver-type aldolase; aldolase B, fructose-bisphosphate; Liver-type aldolase

ALDOB

ALDOB;ALDOB;ALDB;ALDO2;ALDB

ALDOB_HUMAN

364

MGSSHHHHHH SSGLVPRGSH MGSHMAHRFP ALTQEQKKEL SEIAQSIVAN GKGILAADES VGTMGNRLQR IKVENTEENR RQFREILFSV DSSINQSIGG VILFHETLYQ KDSQGKLFRN ILKEKGIVVG IKLDQGGAPL AGTNKETTIQ GLDGLSERCA QYKKDGVDFG KWRAVLRIAD QCPSSLAIQE NANALARYAS ICQQNGLVPI VEPEVIPDGD HDLEHCQYVT EKVLAAVYKA LNDHHVYLEG TLLKPNMVTA GHACTKKYTP EQVAMATVTA LHRTVPAAVP GICFLSGGMS EEDATLNLNA INLCPLPKPW KLSFSYGRAL QASALAAWGG KAANKEATQE AFMKRAMANC QAAKGQYVHT GSSGAASTQS LFTACYTY

> 95% by SDS - PAGE

液体。 在20mM三羟甲基氨基甲烷氯化氢缓冲液(pH值8.0)含1mM DTT, 10% 甘油, 0.1M氯化钠

1毫克/毫升(由布拉德福德Bradford检测确定)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

十二烷基磺酸钠-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli

代谢

Fructose-bisphosphate aldolase B, also known as ALDOB, is one of three known aldolase isoenzymes, and is found in kidney and small adult intestine where it is associated with aldolases A or C. ALDOB catalyzes the reversible cleavage of fructose 1-phosphate into dihydroxyacetone phosphate and glyceraldehyde. It is regulated by the hormones Insulin and glucagon and has been implicated in hereditary fructose intolerance disease. Recombinant human ALDOB protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Caffe A R., et al. (1994) J Comp Neurol. 348:291-297. Dehnes Y., et al. (1998) J Neurosci. 18:3606-3619.

40354205

NP_000026

NM_000035.3

42 kDa (388aa) confirmed by MALDI-TOF

Biosynthesis Of Amino Acids Pathway (790012); Biosynthesis Of Amino Acids Pathway (795174); Carbon Metabolism Pathway (814926); Carbon Metabolism Pathway (817567); Disease Pathway (530764); FOXA2 And FOXA3 Transcription Factor Networks Pathway (137911); Fructose And Mannose Metabolism Pathway (82930); Fructose And Mannose Metabolism Pathway (291); Fructose Catabolism Pathway (106218); Gluconeogenesis Pathway (106204)

Fructose-1,6-bisphosphate aldolase (EC 4.1.2.13) is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Vertebrates have 3 aldolase isozymes which are distinguished by their electrophoretic and catalytic properties. Differences indicate that aldolases A, B, and C are distinct proteins, the products of a family of related 'housekeeping' genes exhibiting developmentally regulated expression of the different isozymes. The developing embryo produces aldolase A, which is produced in even greater amounts in adult muscle where it can be as much as 5% of total cellular protein. In adult liver, kidney and intestine, aldolase A expression is repressed and aldolase B is produced. In brain and other nervous tissue, aldolase A and C are expressed about equally. There is a high degree of homology between aldolase A and C. Defects in ALDOB cause hereditary fructose intolerance. [provided by RefSeq, Dec 2008]

ALDOB: Defects in ALDOB are the cause of hereditary fructose intolerance (HFI). HFI is an autosomal recessive disease that results in an inability to metabolize fructose and related sugars. Complete exclusion of fructose results in dramatic recovery; however, if not treated properly, HFI subjects suffer episodes of hypoglycemia, general ill condition, and risk of death the remainder of life. Belongs to the class I fructose-bisphosphate aldolase family. Protein type: Carbohydrate Metabolism - pentose phosphate pathway; Carbohydrate Metabolism - fructose and mannose; EC 4.1.2.13; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Lyase. Chromosomal Location of Human Ortholog: 9q21.3-q22.2. Cellular Component: microtubule organizing center; cytosol. Molecular Function: identical protein binding; protein binding; cytoskeletal protein binding; fructose-bisphosphate aldolase activity; ATPase binding. Biological Process: fructose 1,6-bisphosphate metabolic process; glycolysis; NADH oxidation; positive regulation of ATPase activity; carbohydrate metabolic process; glucose metabolic process; pathogenesis; fructose catabolic process; fructose metabolic process; gluconeogenesis. Disease: Fructose Intolerance, Hereditary

0.1毫克

310美元

0.5毫克

720