产品简要
公司名称 :
MyBioSource
产品类型 :
蛋白
产品名称 :
TDG, 1-410aa, 人类, 多聚组氨酸标签, 大肠杆菌
目录 :
MBS204223
规格 :
0.1毫克
价格 :
310美元
更多信息或购买 :
产品信息
目录号 :
MBS204223
产品类型 :
重组蛋白
产品全称 :
TDG, 1-410aa, 人类, 多聚组氨酸标签, 大肠杆菌
产品简称 :
TDG
产品名称同义词 :
Thymine-DNA glycosylase; G/T mismatch-specific thymine DNA glycosylase
其他名称 :
Thymine-DNA glycosylase; G/T mismatch-specific thymine DNA glycosylase; G/T mismatch-specific thymine DNA glycosylase; thymine-DNA glycosylase; Thymine-DNA glycosylase; hTDG
产品基因名称 :
TDG
其他基因名称 :
TDG;TDG;hTDG;hTDG
UniProt数据库进入名 :
TDG_HUMAN
序列长度 :
410
序列 :
MGSSHHHHHH SSGLVPRGSH MGSMEAENAG SYSLQQAQAF YTFPFQQLMA EAPNMAVVNE QQMPEEVPAP APAQEPVQEA PKGRKRKPRT TEPKQPVEPK KPVESKKSGK SAKSKEKQEK ITDTFKVKRK VDRFNGVSEA ELLTKTLPDI LTFNLDIVII GINPGLMAAY KGHHYPGPGN HFWKCLFMSG LSEVQLNHMD DHTLPGKYGI GFTNMVERTT PGSKDLSSKE FREGGRILVQ KLQKYQPRIA VFNGKCIYEI FSKEVFGVKV KNLEFGLQPH KIPDTETLCY GMPSSSARCA QFPRAQDKVH YYIKLKDLRD QLKGIERNMD VQEVQYTFDL QLAQEDAKKM AVKEEKYDPG YEAAYGGAYG ENPCSSEPCG FSSNGLIESV ELRGESAFSG IPNGQWMTQS FTDQIPSFSN HCGTQEQEEE SHA
纯度 :
> 90% by SDS - PAGE
形式 :
液体。 在20mM三羟甲基氨基甲烷氯化氢缓冲液(pH值8.0)含0.4尿素, 10% 甘油, 0.1M氯化钠
浓度 :
0.5毫克/毫升(由布拉德福德Bradford检测确定)
储存稳定性 :
Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
检测过的应用 :
十二烷基磺酸钠-PAGE
其它信息1 :
Antigen Species: Human. Tag: His-tag
其他信息2 :
Expression System: E Coli
产品种类 :
Epigenomics
产品描述 :
Thymine-DNA glycosylase, also known as TDG, belongs to the TDG/mug DNA glycosylase family. TDG is a nuclear protein which corrects G/T mismatches to G/C pairs by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and the mispaired thymin. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. Recombinant human TDG protein, fused to His-tag at N-terminus, was expressed in E.coli
产品引用 :
um S., et al. (1998) J Biol Chem. 273:20728-20736. Hardeland u., et al. (2002) EMBO J. 21:1456-1464.
NCBI GI登录号 :
22902196
NCBI登录号 :
AAH37557
NCBI分子量 :
48.4 kDa (433aa)
NCBI信号通路 :
Base Excision Repair Pathway (105838); Base Excision Repair Pathway (83043); Base Excision Repair Pathway (451); Base-Excision Repair, AP Site Formation Pathway (105839); Base-free Sugar-phosphate Removal Via The Single-nucleotide Replacement Pathway (105848); Cleavage Of The Damaged Pyrimidine Pathway (105845); DNA Repair Pathway (105837); Depyrimidination Pathway (105843); Displacement Of DNA Glycosylase By APE1 Pathway (105849); Epigenetic Regulation Of Gene Expression Pathway (1127671)
NCBI总结 :
The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12. [provided by RefSeq, Jul 2008]
UniProt数据库总结 :
TDG: In the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. This enzyme corrects G/T mispairs to G/C pairs. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar- phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T C/T T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine. Belongs to the TDG/mug DNA glycosylase family. Protein type: Nuclear receptor co-regulator; DNA repair, damage; Hydrolase; EC 3.2.2.29. Chromosomal Location of Human Ortholog: 12q24.1. Cellular Component: nucleoplasm; PML body; plasma membrane; nucleus. Molecular Function: protein domain specific binding; protein binding; structure-specific DNA binding; protein homodimerization activity; SUMO binding; protein kinase C binding; DNA N-glycosylase activity; double-stranded DNA binding; damaged DNA binding; transcription factor binding; pyrimidine-specific mismatch base pair DNA N-glycosylase activity; mismatched DNA binding. Biological Process: transcription from RNA polymerase II promoter; embryonic development; mismatch repair; base-excision repair, AP site formation; base-excision repair; depyrimidination; gene expression; negative regulation of transcription from RNA polymerase II promoter; chromatin modification; DNA repair; negative regulation of protein binding; regulation of gene expression, epigenetic
尺寸1 :
0.1毫克
价格1 :
310美元
尺寸2 :
0.5毫克
价格2 :
720
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。