蛋白

VEGF165, 207-371aa, 人类, 多聚组氨酸标签, 大肠杆菌

MBS204593

0.1毫克

310美元

重组蛋白

VEGF165, 207-371aa, 人类, 多聚组氨酸标签, 大肠杆菌

[VEGF165]

[Vascular endothelial growth factor A isoform d; VPF; VEGF; VEGF-A]

[vascular endothelial growth factor A isoform d; Vascular endothelial growth factor A; vascular endothelial growth factor A; vascular endothelial growth factor A; Vascular permeability factor; VPF]

[VEGF165]

[血管内皮生长因子A;血管内皮生长因子A;VPF;血管内皮生长因子;MVCD1;血管内皮生长因子;血管内皮生长因子-A;VPF]

大肠杆菌

371

MGSSHHHHHH SSGLVPRGSH APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR

> 90% by SDS-PAGE

液体。 在20mM三羟甲基氨基甲烷氯化氢(pH值8.5), 50% 甘油, 5mM DTT, 200mM氯化钠, 2mM乙二胺四乙酸

0.5毫克/毫升(由布拉德福德Bradford检测确定)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

十二烷基磺酸钠-PAGE

SDS-Page

Antigen Species: Human. Tag Information: His-tag

Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)

激素&细胞因子

Vascular endothelial growth factor (VEGF) is homodimeric, heparin-binding glycoprotein involved in both angiogenesis and vasculogenesis. VEGF is expressed as multiple alternately spliced isoforms of VEGF121, 165, 189 and 206. VEGF binds to the receptor tyrosine kinases VEGF R1 (Flt-1) and VEGF R2 (KDR/Flk-1) to activate signal transduction and regulate both physiological and pathological angiogenesis. Recombinant human VEGF165 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Yasuji ueda., et al. (2004) Cancer Chemother Pharmacol. 54 (1):16-24. ; Neufeld G., et al. (1999) FASEB J. 13 (1):9-22.

76781483

NP_001020539

NM_001025368.2

P15692

21.3kDa (185aa), conformed by MALDI-TOF

AGE-RAGE Signaling Pathway In Diabetic Complications (1319988); AGE-RAGE Signaling Pathway In Diabetic Complications (1319775); Allograft Rejection Pathway (920963); Amplification And Expansion Of Oncogenic Pathways As Metastatic Traits (1458200); Angiogenesis Pathway (198772); Aryl Hydrocarbon Receptor Pathway (1458217); Axon Guidance Pathway (1270303); Bladder Cancer Pathway (1458294); Bladder Cancer Pathway (83115); Bladder Cancer Pathway (527)

This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome, also known as Crow-Fukase syndrome. Allelic variants of this gene have been associated with microvascular complications of diabetes 1 (MVCD1) and atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been described. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site. [provided by RefSeq, Nov 2015]

Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development ().

0.1毫克

310美元

0.5毫克

720