ELISA/assay

肿瘤坏死因子α(TNFa)酶联免疫吸附试剂盒

MBS2088120

24-Strip-Wells

225美元

酶联免疫吸附试剂盒

肿瘤坏死因子α(TNFa)酶联免疫吸附试剂盒

[肿瘤坏死因子α(TNFa)]

[DIF; TNF-A; TNFSF2; Cachectin; Tumor Necrosis Factor Ligand Superfamily Member 2]

[肿瘤坏死因子α;肿瘤坏死因子α]

[TNFa]

[tnfa]

小鼠, 大鼠

312

This assay has high sensitivity and excellent specificity for detection of TNFa. No significant cross-reactivity or interference between TNFa and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Samples: Rat Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 6.25-400pg/mL. Sensitivity: 2.49pg/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TNFa were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TNFa were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

细胞因子;肿瘤免疫力;感染免疫力

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TNFa in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to TNFa. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to TNFa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TNFa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TNFa in the samples is then determined by comparing the O.D. of the samples to the standard curve.

189409065

NP_001121579.1

NM_001128107.1

24-Strip-Wells

225美元

48-Strip-Wells

365

96-Strip-Wells

490

5x96-Strip-Wells

1935

10x96-Strip-Wells

3485