抗体

大鼠抗人类CD8

MBS212993

0.2毫克

430美元

单克隆

大鼠

未共轭

[YTC182.20]

人类, 牛, 猕猴

流式细胞仪, 流式细胞仪

抗体

大鼠抗人类CD8

[CD8]

[CD8B1, MAL]

[T-cell surface glycoprotein CD8 alpha chain isoform 1; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 alpha chain; CD8 antigen, alpha polypeptide (p32); Leu2 T-lymphocyte antigen; OKT8 T-cell antigen; T cell co-receptor; T-cell antigen Leu2; T-lymphocyte differentiation antigen T8/Leu-2; CD8a molecule; T-lymphocyte differentiation antigen T8/Leu-2; CD_antigen: CD8a]

[CD8]

[CD8A;CD8A;CD8;MAL;p32;Leu2;MAL]

CD8A_HUMAN

单克隆

IgG2b

[YTC182.20]

大鼠

Reacts with: Cynomolgus monkey, Rhesus Monkey. N.B. Antibody reactivity and working conditions may vary between species.

235

纯化。 纯化IgG-液体

IgE浓度1.0毫克/毫升

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch.

Immunohistology Frozen, Flow cytometry (FC/FACS)

Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Flow Cytometry: Minimum Dilution: 1/100; Maximum Dilution: 1/200

试验数据

试验数据

Perservative Stabilisers: 0.09% Sodium Azide. Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Immunogen: Mouse L cells transfected with human CD8 gene. Fusion Partners: Spleen cells from an immunised DA rat were fused with cells of the rat Y3/Ag.1.2.3 myeloma line. Buffer Solution: Phosphate buffered saline. Target Species: Human

Rat anti Human CD8 antibody, clone YTC182.20 recognizes the human CD8 cell surface antigen, a 30/32kD glycoprotein expressed by the cytotoxic/suppressor subset of T lymphocytes, and more weakly by NK cells. Rat anti Human CD8 antibody, clone YTC182.20 recognizes a different epitope from clone YTC141.1.

225007536

NP_001139345.1

NM_001145873.1

30,214 Da

Adaptive Immune System Pathway (366160); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Downstream Signaling In Naive CD8+ T Cells Pathway (138018); Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489); IL12-mediated Signaling Events Pathway (137922); Immune System Pathway (106386)

The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

CD8A: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers. Shown to be expressed as heterodimer on thymocytes and as homodimer on peripheral blood T-lymphocytes. Interacts with the MHC class I HLA-A/B2M dimer. Interacts with LCK in a zinc-dependent manner. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 2p12. Cellular Component: T cell receptor complex; integral to plasma membrane; plasma membrane; extracellular region; external side of plasma membrane. Molecular Function: protein binding; protein homodimerization activity; MHC class I protein binding; coreceptor activity; protein kinase binding. Biological Process: antigen processing and presentation; regulation of immune response; T cell activation; T cell mediated immunity; cytotoxic T cell differentiation; positive regulation of calcium-mediated signaling; immune response; defense response to virus; transmembrane receptor protein tyrosine kinase signaling pathway. Disease: Cd8 Deficiency, Familial

0.2毫克

430美元