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interferon regulatory factor 5 isoform b; Interferon regulatory factor 5; interferon regulatory factor 5; interferon regulatory factor 5
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.
ELISA (EIA), Immunoprecipitation (IP), Immunohistology Paraffin*, Western Blot (WB)
Western Blot: This item detects a band of approximately 58kDa in THP-1 cell lysates. Immunohistology - Paraffin: Minimum Dilution: 1/50; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. . Western Blotting: Minimum Dilution: 1/1000; Maximum Dilution: 1/2000
Perservative Stabilisers: 0.1% Sodium Azide (NaN3) . Preparation: Purified IgG prepared by affinity chromatography on Protein G
Histology Positive Control Tissue: Human Colon. Buffer Solution: Phosphate buffered saline. Target Species: Human
Mouse anti Human IRF5, clone 10T1 recognises human interferon regulatory factor 5 (IRF5), a 58kDa member of the IRF family which acts as a key regulator of interferon gene transcription, and is expressed in B cells and dendritic cells, and is inducible by type I interferon (IFN) in most lymphoid cells. The role of IRF3 and IRF7 as transcriptional mediators of virus- and IFN-induced signaling pathways is well documented. Studies have shown that IRF5 can also regulate IFN gene transcription, but appears to be more restricted in its cellular expression and range of viral stimuli, and has the ability to induce a wide range of chemokines involved in T-lymphocyte recruitment, including RANTES, MCP-1 and IP-10 (Barnes et al. 2002).
NCBI GI登录号 :
Apoptosis Pathway (198797); Cytokine Signaling In Immune System Pathway (366171); Direct P53 Effectors Pathway (137939); Immune System Pathway (106386); Interferon Signaling Pathway (187103); Interferon Alpha/beta Signaling Pathway (187104); Interferon Gamma Signaling Pathway (187106); Regulation Of Toll-like Receptor Signaling Pathway (920955); Senescence And Autophagy Pathway (198780); Toll-like Receptor Signaling Pathway (83076)
This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Multiple transcript variants encoding different isoforms have been found for this gene, and a 30-nt indel polymorphism (SNP rs60344245) can result in loss of a 10-aa segment. [provided by RefSeq, Mar 2010]
IRF5: Transcription factor involved in the induction of interferons IFNA and INFB and inflammatory cytokines upon virus infection. Activated by TLR7 or TLR8 signaling. Homodimer, when phosphorylated. Belongs to the IRF family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: DNA-binding; Transcription factor. Chromosomal Location of Human Ortholog: 7q32. Cellular Component: cytoplasm; nucleus; cytosol. Molecular Function: protein binding; transcription factor activity. Biological Process: transcription, DNA-dependent; positive regulation of interleukin-12 production; positive regulation of apoptosis; response to muramyl dipeptide; response to peptidoglycan; cytokine and chemokine mediated signaling pathway; positive regulation of interferon-beta production; positive regulation of transcription from RNA polymerase II promoter; defense response to virus; positive regulation of interferon-alpha production. Disease: Systemic Lupus Erythematosus, Susceptibility To, 10; Inflammatory Bowel Disease 14
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