产品简要
公司名称 :
MyBioSource
产品类型 :
抗体
产品名称 :
大鼠抗MOUSE CD8
目录 :
MBS214794
规格 :
0.025毫克
价格 :
175美元
克隆性 :
单克隆
宿主 :
大鼠
共轭标签 :
未共轭
克隆名称 :
[YTS105.18]
反应物种 :
小鼠
应用 :
免疫细胞化学, 流式细胞仪, 流式细胞仪
更多信息或购买 :
图像
图像 1 :
MyBioSource MBS214794 图像 1
Published customer image: NKG2D-dependent infiltration of CD8+T cells into tumors. Tumors were collected from mice that had received one of the four standard treatments: control DNA, Dox plus control DNA, IL-12, Dox plus IL-12 (n = 3 per treatment group). (A) Infiltration of NKG2D-positive cells into tumors. Northern blot analysis was performed to detect NKG2D expression in tumors. Ribosomal RNA was used to confirm equal loading among samples. (B) NKG2D/CD8 -positive cells in tumor sections by treatment received. Frozen tumor sections were stained with biotin anti-mouse NKG2D, anti-mouse CD8, or corresponding isotype control antibodies, then with streptavidin-conjugated Alexa fluor 594 or Alexa fluor 488 secondary antibodies. Data shown are representative of three independent experiments. The scale bar is equivalent to 100 um.From: CD8+T cell-specific induction of NKG2D receptor by doxorubicin plus interleukin-12 and its contribution to CD8+T cell accumulation in tumors. Hu J, Zhu S, Xia X, Zhang L, Kleinerman ES, Li S. Mol Cancer. 2014 Feb 24;13:34.
图像 2 :
MyBioSource MBS214794 图像 2
Immunofluorescence staining of a mouse lymph node cryosection with Rat anti mouse CD19, clone 6D5 (MBS212149), green in A and Rat anti Mouse CD8 , red in B. Merged image in C wih nuclei counterstained blue using DAPI. Low power
图像 3 :
MyBioSource MBS214794 图像 3
Staining of mouse spleen cells with Rat anti Mouse CD8 (MBS212026)
产品信息
目录号 :
MBS214794
产品类型 :
抗体
产品全称 :
大鼠抗MOUSE CD8
产品简称 :
[CD8甲]
产品名称同义词 :
[CD8 Alpha; LY-2; Lyt2; Lyt-2]
其他名称 :
[T-cell surface glycoprotein CD8 alpha chain isoform 1; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 alpha chain; Lyt-2.1 lymphocyte differentiation antigen (AA at 100); T-cell surface glycoprotein Lyt-2; CD8 antigen, alpha chain; T-cell surface glycoprotein Lyt-2; CD_antigen: CD8a]
产品基因名称 :
[CD8]
其他基因名称 :
[Cd8a; Cd8a; Ly-2; Ly-B; Ly-35; Lyt-2; BB154331; Lyt-2; Lyt2]
UniProt数据库进入名 :
CD8A_MOUSE
克隆性 :
单克隆
抗体亚型 :
IgG2a
克隆 :
[YTS105.18]
宿主 :
大鼠
反应物种 :
小鼠
序列长度 :
247
特异性 :
Rat anti Mouse CD8, clone YTS105.18 recognizes a non polymorphic epitope on the mouse CD8 alpha chain. This antibody has been reported to block MHC I dependent T cell responses in vitro and in vivo and induces transplantation tolerance in combination with CD4 antibodies.
形式 :
纯化IgG-液体
浓度 :
IgG浓度1毫克/毫升
储存稳定性 :
Store at 4°C or at -20°C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.
检测过的应用 :
Immunohistology-Frozen, Flow cytometry (FC/FACS), Immunofluorescence (IF)
应用笔记 :
Flow Cytometry: Use 10ul of the suggested working dilution to label 10^6 cells in 100ul. Suggested Dilution 1/50-1/100
image1头 :
试验数据
image2头 :
试验数据
image3头 :
试验数据
image4头 :
试验数据
image4描述 :
Immunoperoxidase staining of Mouse lymph node cryosection using Rat anti Mouse CD8alpha antibody followed by horseradish peroxidase conjugated Goat anti Rat IgG (MBS235195) for detection. Low power
image5头 :
试验数据
image5描述 :
Immunofluorescence staining of mouse lymph node cryosection using Rat anti Mouse CD11b antibody , green in A and Rat anti Mouse CD8 antibody , red in B, Merged image in C
其它信息1 :
Target Species: Mouse. Immunogen: Mouse spleen cells. Perservative Stabilisers: 0.09% Sodium Azide. Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. Carrier Free: Yes
其他信息2 :
Buffer Solution: Phosphate buffered saline. Fusion Partners: Spleen cells from an immunised DA rat were fused with cells of the rat Y3/Ag1.2.3 myeloma cell line
NCBI GI登录号 :
124487437
NCBI登录号 :
NP_001074579.1
NCBI基因登录号 :
NM_001081110.2
NCBI信号通路 :
Adaptive Immune System Pathway (1110669); Antigen Processing And Presentation Pathway (83271); Antigen Processing And Presentation Pathway (485); Cell Adhesion Molecules (CAMs) Pathway (83266); Cell Adhesion Molecules (CAMs) Pathway (480); Hematopoietic Cell Lineage Pathway (83275); Hematopoietic Cell Lineage Pathway (489); Immune System Pathway (1110668); Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway (1110695); Primary Immunodeficiency Pathway (83318)
UniProt数据库总结 :
CD8A: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers. Shown to be expressed as heterodimer on thymocytes and as homodimer on peripheral blood T-lymphocytes. Interacts with the MHC class I HLA-A/B2M dimer. Interacts with LCK in a zinc-dependent manner. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Cellular Component: cell surface; membrane; integral to membrane; external side of plasma membrane. Molecular Function: protein binding; protein homodimerization activity; protein kinase binding. Biological Process: cell surface receptor linked signal transduction; T cell activation; immune system process; T cell mediated immunity; positive regulation of calcium-mediated signaling; cytotoxic T cell differentiation; defense response to virus
尺寸1 :
0.025毫克
价格1 :
175美元
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。