产品简要
公司名称 :
MyBioSource
产品类型 :
抗体
产品名称 :
MOUSE抗人类雌激素受体β5
目录 :
MBS219171
规格 :
0.025毫克
价格 :
295美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
25-May
反应物种 :
人类
应用 :
免疫印迹
更多信息或购买 :
产品信息
目录号 :
MBS219171
产品类型 :
抗体
产品全称 :
MOUSE抗人类雌激素受体β5
产品简称 :
雌激素受体β5
其他名称 :
estrogen receptor beta isoform 2; Estrogen receptor beta; estrogen receptor beta; estrogen receptor beta 4; nuclear receptor subfamily 3 group A member 2; estrogen receptor 2 (ER beta); Nuclear receptor subfamily 3 group A member 2
其他基因名称 :
ESR2; ESR2; Erb; ESRB; ESTRB; NR3A2; ER-BETA; ESR-BETA; ESTRB; NR3A2; ER-beta
UniProt数据库进入名 :
ESR2_HUMAN
克隆性 :
单克隆
抗体亚型 :
IgG1
克隆 :
25-May
宿主 :
小鼠
序列长度 :
495
形式 :
纯化。 纯化IgG-液体
浓度 :
IgG浓度1.0毫克/毫升
储存稳定性 :
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.
检测过的应用 :
Immunohistology Paraffin*, Western Blot (WB)
应用笔记 :
Immunohistology - Paraffin: Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
其它信息1 :
Perservative Stabilisers: 0.1% Sodium Azide (NaN3) . Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
其他信息2 :
Histology Positive Control Tissue: Normal colon or colon carcinoma. Buffer Solution: Phosphate buffered saline. Target Species: Human
产品描述 :
Mouse anti human estrogen receptor beta 5, clone 5/25 specifically recognizes human estrogen receptor beta 5 (ERB5), a splice variant of the nuclear receptor hormone ERbeta (ESR2), shown to exist in at least five isoforms (ERB1-ERB5)(Moore et al. 1998). Although only the ERB1 variant appears fully functional, the remaining isoforms have been shown to form heterodimers with ERB1, thereby enhancing transcription (Shaaban et al. 2008). Immunohistochemical studies have revealed a ubiquitous expression of ERB5 in normal colorectal tissue, and to be present in all primary colorectal carcinomas studied, see Wong, N. et al. for details. The detection of estrogen (ER) and progesterone (PR) receptors using immunohistochemical staining of formal fixed paraffin embedded (FFPE) tissue, has gradually replaced ligand binding assays (LBA), to become the most common method for the determination of the ER/PR status of breast tumors. Approximately 75% to 80% of breast tumors have estrogen and/or progesterone receptors, and the presence of these receptors helps determine both the patient's prognosis and the effectiveness of hormonal therapy transcription (Shaaban et al. 2008, Collins et al. 2009).
NCBI GI登录号 :
94538325
NCBI登录号 :
NP_001035365.1
NCBI基因登录号 :
NM_001040275.1
UniProt数据库登录号 :
Q92731
NCBI分子量 :
53,209 Da
NCBI信号通路 :
Estrogen Signaling Pathway (799177); Estrogen Signaling Pathway (799197); Gene Expression Pathway (105937); Generic Transcription Pathway (105938); Integrated Pancreatic Cancer Pathway (711360); Nuclear Receptor Transcription Pathway (105979); Nuclear Receptors Pathway (198848); Ovarian Infertility Genes Pathway (198801); Plasma Membrane Estrogen Receptor Signaling Pathway (138009); Prolactin Signaling Pathway (814182)
NCBI总结 :
This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized. [provided by RefSeq, Jul 2008]
UniProt数据库总结 :
ER-beta: a nuclear hormone receptor and transcription factor. Binds and activated by estrogen. Regulates gene expression and affects cellular proliferation and differentiation in target tissues. Binds estrogens with an affinity similar to that of ER-alpha, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Eight alternatively-spliced isoforms have been described. Isoform beta-cx lacks ligand binding ability and has no or only very low ERE binding activity resulting in the loss of ligand-dependent transactivation ability. Protein type: DNA-binding; Nuclear receptor. Chromosomal Location of Human Ortholog: 14q23.2. Cellular Component: nucleoplasm; mitochondrion; extracellular region; nucleus. Molecular Function: ligand-dependent nuclear receptor activity; receptor antagonist activity; protein binding; estrogen receptor activity; enzyme binding; DNA binding; zinc ion binding; transcription coactivator activity; steroid hormone receptor activity; estrogen response element binding; transcription factor activity; steroid binding. Biological Process: transcription initiation from RNA polymerase II promoter; estrogen receptor signaling pathway; induction of apoptosis by hormones; neuron migration; vagina development; negative regulation of transcription from RNA polymerase II promoter; uterus development; signal transduction; ovarian follicle development; cell-cell signaling; regulation of transcription, DNA-dependent; positive regulation of transcription factor activity; steroid hormone mediated signaling; gene expression; brain development; negative regulation of cell growth; negative regulation of epithelial cell proliferation
尺寸1 :
0.025毫克
价格1 :
295美元
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。