免疫印迹, 酶联免疫吸附测定, 酶免疫法
extracellular sulfatase Sulf-2 isoform 1; Extracellular sulfatase Sulf-2; extracellular sulfatase Sulf-2; sulfatase 2
Sulf2; Sulf2; MSulf-2; AU020235; mKIAA1247; 2010004N24Rik; mSulf-2
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.
ELISA (EIA), Immunohistology Paraffin*, Western Blot (WB)
Western Blot: Mouse anti Human sulfatase 2 antibody, clone 2B4 detects a major band of ~50kDa in Sulf-2 transfected HEK293 cell lysates. A band at 135kDa (unprocessed protein), and a fragment at 37kDa, may also be present. Immunohistology - Paraffin: Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Perservative Stabilisers: 0.09% Sodium Azide. Preparation: Purified IgG prepared by affinity chromatography on Protein G.
Immunogen: Recombinant human Sulf-2. Histology Positive Control Tissue: Sulf-2 transfected HEK29 cells or non-small-cell lung carcinoma. Buffer Solution: Phosphate buffered saline. Target Species: Human
Mouse anti Human Sulfatase 2 (C-Terminal) antibody, clone 2B4 recognizes an epitope within the C-terminal (CT) subunit of Sulfatase 2 (Sulf-2). Sulf-2 is a novel extracellular heparan sulfate 6-O-endosulfatase, which selectively removes the critical sulfate group from the polysaccharide side chain of heparan sulfate proteoglycans, major components of the extracellular matrix, involved in cell differentiation, proliferation and migration. Sulf-2 acts as a modulator of several signalling proteins including Wnt proteins, bone morphogenetic proteins (BMPs) and fibroblast growth factors (FGFs), and is a direct transcriptional target of p53. Studies have linked Sulf-2 with several cancers, including breast, hepatocellular, and pancreatic cancer, and recently with non-small-cell lung carcinomas (Lemjabbar-Alaoui et al. 2010). Mature human Sulf-2 is a heterodimer consisting of a 50kDa C-terminal subunit, essential for both glucosamine 6-O-sulfate-endosulfatase and arylsulfatase activity, and a 75kDa N-terminal subunit which contains the catalytic centre. Mouse anti Human Sulfatase 2 (C-Terminal) antibody, clone 2B4recognizes Sulfatase 2, showing no cross-reactivity with Sulfatase 1 in either human or mouse.
NCBI GI登录号 :
SULF2: Exhibits arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity. It can remove sulfate from the C-6 position of glucosamine within specific subregions of intact heparin. Belongs to the sulfatase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.1.6.-; Secreted; Hydrolase; Secreted, signal peptide. Cellular Component: Golgi apparatus; extracellular space; cell surface; endoplasmic reticulum; plasma membrane. Molecular Function: arylsulfatase activity; N-acetylglucosamine-6-sulfatase activity; hydrolase activity; sulfuric ester hydrolase activity; metal ion binding; calcium ion binding; catalytic activity; alkyl sulfatase activity. Biological Process: sulfur metabolic process; negative regulation of fibroblast growth factor receptor signaling pathway; metabolic process; cartilage development; embryonic skeletal development; glomerular filtration; esophagus smooth muscle contraction; glomerular basement membrane development; heparan sulfate proteoglycan metabolic process; chondrocyte development; kidney development; positive regulation of Wnt receptor signaling pathway
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308