抗体

兔抗人类PDGF BB HOMODIMER

MBS221731

0.1毫克

540美元

多克隆

兔

未共轭

免疫印迹, 酶联免疫吸附测定, 酶免疫法

抗体

兔抗人类PDGF BB HOMODIMER

PDGF

platelet-derived growth factor subunit B isoform 1 preproprotein; Platelet-derived growth factor subunit B; platelet-derived growth factor subunit B; PDGF subunit B; PDGF, B chain; becaplermin; platelet-derived growth factor 2; platelet-derived growth factor B chain; platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog); platelet-derived growth factor, beta polypeptide (oncogene SIS); proto-oncogene c-Sis; platelet-derived growth factor beta polypeptide; PDGF-2; Platelet-derived growth factor B chain; Platelet-derived growth factor beta polypeptide; Proto-oncogene c-Sis; INN: Becaplermin

PDGFB; PDGFB; SIS; SSV; IBGC5; PDGF2; c-sis; PDGF-2; PDGF2; SIS; PDGF subunit B

PDGFB_HUMAN

多克隆

IgG

兔

241

Purified. Purified IgG - lyophilised

IgG concentration 1.0mg/ml after reconstitution

Prior to reconstitution store at 4 degree C. After reconstitution store at -20 degree C. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of reconstitution.

ELISA (EIA), Functional Assays (FN), Immunohistology Paraffin*, Western Blot (WB)

ELISA: Minimum Dilution: 0.5; Maximum Dilution: 2.0ug/ml;. Functional Assays: Minimum Dilution: 0.03; Maximum Dilution: 0.04ug/ml;. Immunohistology - Paraffin: Application Note: This product requires enzyme mediated antigen retrieval prior to staining of parrafin sections. Proteinase K is recomended for this purpose. . Western Blotting: Minimum Dilution: 0.1; Maximum Dilution: 0.2ug/ml

Reconstitution: Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.

Buffer Solution: Phosphate buffered saline. Antiserum Preparation: Antisera to human PDGF-BB were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography. Target Species: Human

Rabbit anti Human PDGF BB Homodimer antibody recognizes human PDGF-BB (Platelet derived growth factor B chain), a disulphide-linked beta-chain homodimer and member of the PDGF family, released from platelet alpha-granules following trauma/platelet activation. Naturally occurring active forms of PDGF exist as dimers of two alpha chains (AA), two beta chains (BB), or a combination of both chains (AB), all of which signal through the high-affinity PDGF-Ralpha receptor, and the latter two of which can also signal through the PDGF-Rbeta receptor. PDGFs are potent mitogens for a variety of cells including those of the connective tissue, bone and cartilage, blood and smooth muscle cells, and are involved in several biological processes including chemotaxis, hyperplasia and embryonic neurogenesis. The expression of PDGF-BB is associated with autocrine growth stimulation and the formation of various tumour types, with several studies focusing on dermatofibrosarcoma protuberans (DP), an infiltrating skin tumour arising from a translocation occurring between chromosome 22 (the location of the PDGFB gene) and chromosome 17 (the location of the COL1A1 gene), which results in the generation of the COL1A1-PDGFB chimeric protein.

4505681

NP_002599.1

NM_002608.2

P01127

25,502 Da

Adaptive Immune System Pathway (366160); Angiogenesis Pathway (198772); Choline Metabolism In Cancer Pathway (1059539); Choline Metabolism In Cancer Pathway (1084232); Constitutive PI3K/AKT Signaling In Cancer Pathway (685535); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); DAP12 Interactions Pathway (685549); DAP12 Signaling Pathway (685550); Disease Pathway (530764)

The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 17, at sites where this gene and that for collagen type 1, alpha 1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Feb 2013]

PDGFB: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA. A chromosomal aberration involving PDGFB is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGFB. Belongs to the PDGF/VEGF growth factor family. Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Oncoprotein; Secreted. Chromosomal Location of Human Ortholog: 22q13.1. Cellular Component: Golgi membrane; extracellular space; cell surface; endoplasmic reticulum lumen; basolateral plasma membrane; cytoplasm; extracellular region. Molecular Function: collagen binding; identical protein binding; protein binding; protein homodimerization activity; growth factor activity; protein heterodimerization activity; platelet-derived growth factor binding; platelet-derived growth factor receptor binding; superoxide-generating NADPH oxidase activator activity; chemoattractant activity. Biological Process: extracellular matrix organization and biogenesis; positive regulation of cyclin-dependent protein kinase activity; nerve growth factor receptor signaling pathway; heart development; positive regulation of transcription, DNA-dependent; positive regulation of fibroblast growth factor receptor signaling pathway; cell projection biogenesis; protein amino acid phosphorylation; monocyte chemotaxis; positive regulation of MAP kinase activity; positive regulation of fibroblast proliferation; positive chemotaxis; transforming growth factor beta receptor signaling pathway; positive regulation of mitotic cell cycle, embryonic; cell growth; substrate-bound cell migration; response to drug; platelet activation; fibroblast growth factor receptor signaling pathway; positive regulation of mitosis; positive regulation of chemotaxis; activation of protein kinase B; positive regulation of blood vessel endothelial cell migration; positive regulation of protein amino acid autophosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of peptidyl-tyrosine phosphorylation; activation of protein kinase activity; blood vessel morphogenesis; positive regulation of endothelial cell proliferation; negative regulation of transcription, DNA-dependent; actin cytoskeleton organization and biogenesis; embryonic placenta development; peptidyl-tyrosine phosphorylation; positive regulation of smooth muscle cell proliferation; platelet-derived growth factor receptor signaling pathway; positive regulation of smooth muscle cell migration; response to estradiol stimulus; response to insulin stimulus; platelet degranulation; positive regulation of MAPKKK cascade; positive regulation of glomerular filtration; positive regulation of cell proliferation; response to wounding; hemopoiesis; DNA replication; negative regulation of cell migration; epidermal growth factor receptor signaling pathway; phosphoinositide-mediated signaling; negative regulation of phosphatidylinositol biosynthetic process; positive regulation of phosphoinositide 3-kinase activity; peptidyl-serine phosphorylation; response to hypoxia; innate immune response; blood coagulation; positive regulation of DNA replication; positive regulation of cell migration. Disease: Meningioma, Familial, Susceptibility To; Dermatofibrosarcoma Protuberans; Basal Ganglia Calcification, Idiopathic, 5; Basal Ganglia Calcification, Idiopathic, 1

0.1毫克

540美元